HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of <i>Anethum Graveolens</i>, <i>Pimpinella Anisum</i>, and <i>Ferulago Campestris</i>
Umbelliprenin has recently been shown to have great potential as a skin whitening agent. Wishing to investigate the same effect in plant species known to biosynthesize this coumarin, three plants belonging to the Apiaceae family, namely <i>Anethum graveolens</i> L. (dill), <i>Pimpi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/3/501 |
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| author | Vito Alessandro Taddeo Francesco Epifano Francesca Preziuso Serena Fiorito Nicolas Caron Arnaud Rives Philippe de Medina Marc Poirot Sandrine Silvente-Poirot Salvatore Genovese |
| author_facet | Vito Alessandro Taddeo Francesco Epifano Francesca Preziuso Serena Fiorito Nicolas Caron Arnaud Rives Philippe de Medina Marc Poirot Sandrine Silvente-Poirot Salvatore Genovese |
| author_sort | Vito Alessandro Taddeo |
| collection | DOAJ |
| description | Umbelliprenin has recently been shown to have great potential as a skin whitening agent. Wishing to investigate the same effect in plant species known to biosynthesize this coumarin, three plants belonging to the Apiaceae family, namely <i>Anethum graveolens</i> L. (dill), <i>Pimpinella anisum</i> L. (anise), and <i>Ferulago campestris</i> (Besser) Grecescu (field ferula) were screened by HPLC analysis for their respective content of umbelliprenin in extracts obtained with different solvent mixtures and by maceration and ultrasound-assisted processes. EtOH was shown to be the best solvent, providing umbelliprenin yields ranging from 1.7% to 14.4% (with respect to the total amount of extract obtained). Extracts with the highest content of this farnesyloxycoumarin were then assayed as modulators of melanogenesis in cultured murine Melan A cells employing the same umbelliprenin obtained by chemical synthesis as the reference. A parallelism between the content of the coumarin and the recorded depigmenting effect (60% for the EtOH extract of <i>F. campestris</i> as the best value) was revealed for all plants extracts when applied at a dose of 100 μg/mL. Our results demonstrate that the same potential of umbelliprenin can be ascribed also to umbelliprenin-enriched plant extracts which reinforces enforce the widespread use of phyto-preparations for cosmetic purposes (e.g., <i>A. graveolens</i>). |
| first_indexed | 2024-12-13T14:14:18Z |
| format | Article |
| id | doaj.art-a3f38b3ec40b4da3b2b7ed73a3076b6c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T14:14:18Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a3f38b3ec40b4da3b2b7ed73a3076b6c2022-12-21T23:42:22ZengMDPI AGMolecules1420-30492019-01-0124350110.3390/molecules24030501molecules24030501HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of <i>Anethum Graveolens</i>, <i>Pimpinella Anisum</i>, and <i>Ferulago Campestris</i>Vito Alessandro Taddeo0Francesco Epifano1Francesca Preziuso2Serena Fiorito3Nicolas Caron4Arnaud Rives5Philippe de Medina6Marc Poirot7Sandrine Silvente-Poirot8Salvatore Genovese9Dipartimento di Farmacia, Università “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti Scalo (CH), ItalyDipartimento di Farmacia, Università “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti Scalo (CH), ItalyDipartimento di Farmacia, Università “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti Scalo (CH), ItalyDipartimento di Farmacia, Università “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti Scalo (CH), ItalySociété de Biotechnologie médicale, Affichem S.A., 9 Rue Saint Joseph, 31400 Toulouse, FranceSociété de Biotechnologie médicale, Affichem S.A., 9 Rue Saint Joseph, 31400 Toulouse, FranceCholesterol Metabolism and Therapeutic Innovations, Cancer Research Center of Toulouse (CRCT), UMR 1037, INSERM-Université de Toulouse, 31400 Toulouse, FranceCholesterol Metabolism and Therapeutic Innovations, Cancer Research Center of Toulouse (CRCT), UMR 1037, INSERM-Université de Toulouse, 31400 Toulouse, FranceCholesterol Metabolism and Therapeutic Innovations, Cancer Research Center of Toulouse (CRCT), UMR 1037, INSERM-Université de Toulouse, 31400 Toulouse, FranceDipartimento di Farmacia, Università “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti Scalo (CH), ItalyUmbelliprenin has recently been shown to have great potential as a skin whitening agent. Wishing to investigate the same effect in plant species known to biosynthesize this coumarin, three plants belonging to the Apiaceae family, namely <i>Anethum graveolens</i> L. (dill), <i>Pimpinella anisum</i> L. (anise), and <i>Ferulago campestris</i> (Besser) Grecescu (field ferula) were screened by HPLC analysis for their respective content of umbelliprenin in extracts obtained with different solvent mixtures and by maceration and ultrasound-assisted processes. EtOH was shown to be the best solvent, providing umbelliprenin yields ranging from 1.7% to 14.4% (with respect to the total amount of extract obtained). Extracts with the highest content of this farnesyloxycoumarin were then assayed as modulators of melanogenesis in cultured murine Melan A cells employing the same umbelliprenin obtained by chemical synthesis as the reference. A parallelism between the content of the coumarin and the recorded depigmenting effect (60% for the EtOH extract of <i>F. campestris</i> as the best value) was revealed for all plants extracts when applied at a dose of 100 μg/mL. Our results demonstrate that the same potential of umbelliprenin can be ascribed also to umbelliprenin-enriched plant extracts which reinforces enforce the widespread use of phyto-preparations for cosmetic purposes (e.g., <i>A. graveolens</i>).https://www.mdpi.com/1420-3049/24/3/501aniseapiaceaedillfield ferulamelaninmelanogenesisoxyprenylated coumarinsskin whitening effectumbelliprenin |
| spellingShingle | Vito Alessandro Taddeo Francesco Epifano Francesca Preziuso Serena Fiorito Nicolas Caron Arnaud Rives Philippe de Medina Marc Poirot Sandrine Silvente-Poirot Salvatore Genovese HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of <i>Anethum Graveolens</i>, <i>Pimpinella Anisum</i>, and <i>Ferulago Campestris</i> Molecules anise apiaceae dill field ferula melanin melanogenesis oxyprenylated coumarins skin whitening effect umbelliprenin |
| title | HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of <i>Anethum Graveolens</i>, <i>Pimpinella Anisum</i>, and <i>Ferulago Campestris</i> |
| title_full | HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of <i>Anethum Graveolens</i>, <i>Pimpinella Anisum</i>, and <i>Ferulago Campestris</i> |
| title_fullStr | HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of <i>Anethum Graveolens</i>, <i>Pimpinella Anisum</i>, and <i>Ferulago Campestris</i> |
| title_full_unstemmed | HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of <i>Anethum Graveolens</i>, <i>Pimpinella Anisum</i>, and <i>Ferulago Campestris</i> |
| title_short | HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of <i>Anethum Graveolens</i>, <i>Pimpinella Anisum</i>, and <i>Ferulago Campestris</i> |
| title_sort | hplc analysis and skin whitening effects of umbelliprenin containing extracts of i anethum graveolens i i pimpinella anisum i and i ferulago campestris i |
| topic | anise apiaceae dill field ferula melanin melanogenesis oxyprenylated coumarins skin whitening effect umbelliprenin |
| url | https://www.mdpi.com/1420-3049/24/3/501 |
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