Effective biosynthesis of 2,5-furandicarboxylic acid from 5-hydroxymethylfurfural via a bi-enzymatic cascade system using bacterial laccase and fungal alcohol oxidase
Abstract Background As a cost-effective and eco-friendly approach, biocatalysis has great potential for the transformation of 5-hydroxymethylfurfural (HMF) into 2,5-furandicarboxylic acid (FDCA). However, the compatibility of each enzyme in the cascade reaction limits the transformation efficiency o...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-11-01
|
| Series: | Biotechnology for Biofuels and Bioproducts |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-023-02406-z |
| _version_ | 1797637063284097024 |
|---|---|
| author | Fan Yang Jiashu Liu Bianxia Li Huanan Li Zhengbing Jiang |
| author_facet | Fan Yang Jiashu Liu Bianxia Li Huanan Li Zhengbing Jiang |
| author_sort | Fan Yang |
| collection | DOAJ |
| description | Abstract Background As a cost-effective and eco-friendly approach, biocatalysis has great potential for the transformation of 5-hydroxymethylfurfural (HMF) into 2,5-furandicarboxylic acid (FDCA). However, the compatibility of each enzyme in the cascade reaction limits the transformation efficiency of HMF to FDCA. Results Coupled with an alcohol oxidase from Colletotrichum gloeosporioides (CglAlcOx), this study aims to study the potential of bacterial laccase from Bacillus pumilus (BpLac) in an enzymatic cascade for 2,5-furandicarboxylic acid (FDCA) biosynthesis from 5-hydroxymethylfurfural (HMF). BpLac showed 100% selectivity for HMF oxidation and generated 5-hydroxymethyl-2-furancarboxylic acid (HMFCA). CglAlcOx was capable of oxidizing HMFCA to 2-formyl-5-furancarboxylic acid (FFCA). Both BpLac and CglAlcOx could oxidize FFCA to FDCA. At the 5 mM scale, a complete transformation of HMF with a 97.5% yield of FDCA was achieved by coupling BpLac with CglAlcOx in the cascade reaction. The FDCA productivity in the reaction was 5.3 mg/L/h. Notably, BpLac could alleviate the inhibitory effect of FFCA on CglAlcOx activity and boost the transformation efficiency of HMF to FDCA. Moreover, the reaction was scaled up to 40 times the volume, and FDCA titer reached 2.6 mM with a yield of 58.77% at 168 h. Conclusions This work provides a candidate and novel insight for better design of an enzymatic cascade in FDCA production. |
| first_indexed | 2024-03-11T12:43:59Z |
| format | Article |
| id | doaj.art-a3f7d622332a47a19a444c340572a2b3 |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-03-11T12:43:59Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-a3f7d622332a47a19a444c340572a2b32023-11-05T12:10:11ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542023-11-0116111310.1186/s13068-023-02406-zEffective biosynthesis of 2,5-furandicarboxylic acid from 5-hydroxymethylfurfural via a bi-enzymatic cascade system using bacterial laccase and fungal alcohol oxidaseFan Yang0Jiashu Liu1Bianxia Li2Huanan Li3Zhengbing Jiang4State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei UniversityAbstract Background As a cost-effective and eco-friendly approach, biocatalysis has great potential for the transformation of 5-hydroxymethylfurfural (HMF) into 2,5-furandicarboxylic acid (FDCA). However, the compatibility of each enzyme in the cascade reaction limits the transformation efficiency of HMF to FDCA. Results Coupled with an alcohol oxidase from Colletotrichum gloeosporioides (CglAlcOx), this study aims to study the potential of bacterial laccase from Bacillus pumilus (BpLac) in an enzymatic cascade for 2,5-furandicarboxylic acid (FDCA) biosynthesis from 5-hydroxymethylfurfural (HMF). BpLac showed 100% selectivity for HMF oxidation and generated 5-hydroxymethyl-2-furancarboxylic acid (HMFCA). CglAlcOx was capable of oxidizing HMFCA to 2-formyl-5-furancarboxylic acid (FFCA). Both BpLac and CglAlcOx could oxidize FFCA to FDCA. At the 5 mM scale, a complete transformation of HMF with a 97.5% yield of FDCA was achieved by coupling BpLac with CglAlcOx in the cascade reaction. The FDCA productivity in the reaction was 5.3 mg/L/h. Notably, BpLac could alleviate the inhibitory effect of FFCA on CglAlcOx activity and boost the transformation efficiency of HMF to FDCA. Moreover, the reaction was scaled up to 40 times the volume, and FDCA titer reached 2.6 mM with a yield of 58.77% at 168 h. Conclusions This work provides a candidate and novel insight for better design of an enzymatic cascade in FDCA production.https://doi.org/10.1186/s13068-023-02406-zBacterial laccaseAlcohol oxidaseEnzymatic cascadeBiocatalysis2,5-Furandicarboxylic acid |
| spellingShingle | Fan Yang Jiashu Liu Bianxia Li Huanan Li Zhengbing Jiang Effective biosynthesis of 2,5-furandicarboxylic acid from 5-hydroxymethylfurfural via a bi-enzymatic cascade system using bacterial laccase and fungal alcohol oxidase Biotechnology for Biofuels and Bioproducts Bacterial laccase Alcohol oxidase Enzymatic cascade Biocatalysis 2,5-Furandicarboxylic acid |
| title | Effective biosynthesis of 2,5-furandicarboxylic acid from 5-hydroxymethylfurfural via a bi-enzymatic cascade system using bacterial laccase and fungal alcohol oxidase |
| title_full | Effective biosynthesis of 2,5-furandicarboxylic acid from 5-hydroxymethylfurfural via a bi-enzymatic cascade system using bacterial laccase and fungal alcohol oxidase |
| title_fullStr | Effective biosynthesis of 2,5-furandicarboxylic acid from 5-hydroxymethylfurfural via a bi-enzymatic cascade system using bacterial laccase and fungal alcohol oxidase |
| title_full_unstemmed | Effective biosynthesis of 2,5-furandicarboxylic acid from 5-hydroxymethylfurfural via a bi-enzymatic cascade system using bacterial laccase and fungal alcohol oxidase |
| title_short | Effective biosynthesis of 2,5-furandicarboxylic acid from 5-hydroxymethylfurfural via a bi-enzymatic cascade system using bacterial laccase and fungal alcohol oxidase |
| title_sort | effective biosynthesis of 2 5 furandicarboxylic acid from 5 hydroxymethylfurfural via a bi enzymatic cascade system using bacterial laccase and fungal alcohol oxidase |
| topic | Bacterial laccase Alcohol oxidase Enzymatic cascade Biocatalysis 2,5-Furandicarboxylic acid |
| url | https://doi.org/10.1186/s13068-023-02406-z |
| work_keys_str_mv | AT fanyang effectivebiosynthesisof25furandicarboxylicacidfrom5hydroxymethylfurfuralviaabienzymaticcascadesystemusingbacteriallaccaseandfungalalcoholoxidase AT jiashuliu effectivebiosynthesisof25furandicarboxylicacidfrom5hydroxymethylfurfuralviaabienzymaticcascadesystemusingbacteriallaccaseandfungalalcoholoxidase AT bianxiali effectivebiosynthesisof25furandicarboxylicacidfrom5hydroxymethylfurfuralviaabienzymaticcascadesystemusingbacteriallaccaseandfungalalcoholoxidase AT huananli effectivebiosynthesisof25furandicarboxylicacidfrom5hydroxymethylfurfuralviaabienzymaticcascadesystemusingbacteriallaccaseandfungalalcoholoxidase AT zhengbingjiang effectivebiosynthesisof25furandicarboxylicacidfrom5hydroxymethylfurfuralviaabienzymaticcascadesystemusingbacteriallaccaseandfungalalcoholoxidase |