Association of the rs1126616 and rs9138 Variants in the <i>SPP1</i> Gene among Mexican Patients with Systemic Lupus Erythematosus and Lupus Nephritis
Systemic lupus erythematosus (SLE) is a multisystem disease considered a prototype of the main autoimmune disease and presents serious complications, such as lupus nephritis (LN), which generates a significant impact on morbidity and mortality. The <i>SPP1</i> gene encodes the osteoponti...
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/2/1000 |
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| author | Alicia Rivera-Cameras Martha Patricia Gallegos-Arreola María Cristina Morán-Moguel Mario Salazar-Páramo Miriam Fabiola Alcaraz-López Gustavo Echeverría-González Jorge Fernando Topete-Reyes Sergio Adalberto Franco-Chávez Ingrid Patricia Dávalos-Rodríguez |
| author_facet | Alicia Rivera-Cameras Martha Patricia Gallegos-Arreola María Cristina Morán-Moguel Mario Salazar-Páramo Miriam Fabiola Alcaraz-López Gustavo Echeverría-González Jorge Fernando Topete-Reyes Sergio Adalberto Franco-Chávez Ingrid Patricia Dávalos-Rodríguez |
| author_sort | Alicia Rivera-Cameras |
| collection | DOAJ |
| description | Systemic lupus erythematosus (SLE) is a multisystem disease considered a prototype of the main autoimmune disease and presents serious complications, such as lupus nephritis (LN), which generates a significant impact on morbidity and mortality. The <i>SPP1</i> gene encodes the osteopontin (OPN) protein, which plays a crucial role in the regulation of inflammation and immunity. The variants rs1126616 and rs9138 of this gene have been associated with the inflammatory response. The study aims to analyze the association of the rs1126616 and rs9138 variants of the <i>SPP1</i> gene in SLE Mexican-Mestizo patients without LN (SLE-LN). In this cross-sectional study, a total of 171 genomic DNA samples from SLE patients were clinically confirmed, of which 111 were SLE without LN, 60 were SLE with LN, and 100 healthy individuals were included as reference group. The rs1126616 variant was genotyped using PCR-RFLPs, and the rs9138 variant was genotyped using qPCR TaqMan. The TT genotype, the recessive model [OR 2.76 (95% CI 1.31–5.82), <i>p</i> = 0.011], and the T allele [OR 2.0 (95% CI 1.26–3.16), <i>p</i> = 0.003] of the rs1126616 variant are risk factors for SLE with LN. By contrast, the rs9138 variant did not show statistically significant differences among SLE patients stratified by LN. In our study of SLE Mexican-Mestizo patients with and without NL, demographic and clinical characteristics do not differ from other SLE populations, and the TT genotype of the rs1126616 variant of the <i>SPP1</i> gene confers a risk factor for the presentation of LN. Otherwise, the rs9138 variant did not show association with NL. |
| first_indexed | 2024-03-08T09:54:04Z |
| format | Article |
| id | doaj.art-a3fdf553b90c4c4e98cd221da925e2b5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T09:54:04Z |
| publishDate | 2024-01-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a3fdf553b90c4c4e98cd221da925e2b52024-01-29T13:56:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-01252100010.3390/ijms25021000Association of the rs1126616 and rs9138 Variants in the <i>SPP1</i> Gene among Mexican Patients with Systemic Lupus Erythematosus and Lupus NephritisAlicia Rivera-Cameras0Martha Patricia Gallegos-Arreola1María Cristina Morán-Moguel2Mario Salazar-Páramo3Miriam Fabiola Alcaraz-López4Gustavo Echeverría-González5Jorge Fernando Topete-Reyes6Sergio Adalberto Franco-Chávez7Ingrid Patricia Dávalos-Rodríguez8División de Genética, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara 44340, MexicoDivisión de Genética, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara 44340, MexicoDepartamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, MexicoDepartamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, MexicoServicio de Reumatología, Hospital General Regional 46, Instituto Mexicano del Seguro Social, Guadalajara 44910, MexicoServicio de Reumatología, Unidad Médica de Alta Especialidad, Hospital de Especialidades, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social, Guadalajara 44329, MexicoServicio de Nefrología, Hospital General Regional 46, Instituto Mexicano del Seguro Social, Guadalajara 44910, MexicoDepartamento de Salud Pública, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, MexicoDivisión de Genética, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara 44340, MexicoSystemic lupus erythematosus (SLE) is a multisystem disease considered a prototype of the main autoimmune disease and presents serious complications, such as lupus nephritis (LN), which generates a significant impact on morbidity and mortality. The <i>SPP1</i> gene encodes the osteopontin (OPN) protein, which plays a crucial role in the regulation of inflammation and immunity. The variants rs1126616 and rs9138 of this gene have been associated with the inflammatory response. The study aims to analyze the association of the rs1126616 and rs9138 variants of the <i>SPP1</i> gene in SLE Mexican-Mestizo patients without LN (SLE-LN). In this cross-sectional study, a total of 171 genomic DNA samples from SLE patients were clinically confirmed, of which 111 were SLE without LN, 60 were SLE with LN, and 100 healthy individuals were included as reference group. The rs1126616 variant was genotyped using PCR-RFLPs, and the rs9138 variant was genotyped using qPCR TaqMan. The TT genotype, the recessive model [OR 2.76 (95% CI 1.31–5.82), <i>p</i> = 0.011], and the T allele [OR 2.0 (95% CI 1.26–3.16), <i>p</i> = 0.003] of the rs1126616 variant are risk factors for SLE with LN. By contrast, the rs9138 variant did not show statistically significant differences among SLE patients stratified by LN. In our study of SLE Mexican-Mestizo patients with and without NL, demographic and clinical characteristics do not differ from other SLE populations, and the TT genotype of the rs1126616 variant of the <i>SPP1</i> gene confers a risk factor for the presentation of LN. Otherwise, the rs9138 variant did not show association with NL.https://www.mdpi.com/1422-0067/25/2/1000Lupus nephritisosteopontin<i>SPP1</i> genesystemic lupus erythematosus |
| spellingShingle | Alicia Rivera-Cameras Martha Patricia Gallegos-Arreola María Cristina Morán-Moguel Mario Salazar-Páramo Miriam Fabiola Alcaraz-López Gustavo Echeverría-González Jorge Fernando Topete-Reyes Sergio Adalberto Franco-Chávez Ingrid Patricia Dávalos-Rodríguez Association of the rs1126616 and rs9138 Variants in the <i>SPP1</i> Gene among Mexican Patients with Systemic Lupus Erythematosus and Lupus Nephritis International Journal of Molecular Sciences Lupus nephritis osteopontin <i>SPP1</i> gene systemic lupus erythematosus |
| title | Association of the rs1126616 and rs9138 Variants in the <i>SPP1</i> Gene among Mexican Patients with Systemic Lupus Erythematosus and Lupus Nephritis |
| title_full | Association of the rs1126616 and rs9138 Variants in the <i>SPP1</i> Gene among Mexican Patients with Systemic Lupus Erythematosus and Lupus Nephritis |
| title_fullStr | Association of the rs1126616 and rs9138 Variants in the <i>SPP1</i> Gene among Mexican Patients with Systemic Lupus Erythematosus and Lupus Nephritis |
| title_full_unstemmed | Association of the rs1126616 and rs9138 Variants in the <i>SPP1</i> Gene among Mexican Patients with Systemic Lupus Erythematosus and Lupus Nephritis |
| title_short | Association of the rs1126616 and rs9138 Variants in the <i>SPP1</i> Gene among Mexican Patients with Systemic Lupus Erythematosus and Lupus Nephritis |
| title_sort | association of the rs1126616 and rs9138 variants in the i spp1 i gene among mexican patients with systemic lupus erythematosus and lupus nephritis |
| topic | Lupus nephritis osteopontin <i>SPP1</i> gene systemic lupus erythematosus |
| url | https://www.mdpi.com/1422-0067/25/2/1000 |
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