Evaluation of the Function of ASFV Gene E66L in the Process of Virus Replication and Virulence in Swine
African swine fever virus (ASFV) is the etiological agent of an economically important disease of swine currently affecting large areas of Africa, Eurasia and the Caribbean. ASFV has a complex structure harboring a large dsDNA genome which encodes for more than 160 proteins. One of the proteins, E66...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/2/566 |
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| author | Elizabeth Ramirez-Medina Elizabeth A. Vuono Ayushi Rai Nallely Espinoza Alyssa Valladares Edward Spinard Lauro Velazquez-Salinas Douglas P. Gladue Manuel V. Borca |
| author_facet | Elizabeth Ramirez-Medina Elizabeth A. Vuono Ayushi Rai Nallely Espinoza Alyssa Valladares Edward Spinard Lauro Velazquez-Salinas Douglas P. Gladue Manuel V. Borca |
| author_sort | Elizabeth Ramirez-Medina |
| collection | DOAJ |
| description | African swine fever virus (ASFV) is the etiological agent of an economically important disease of swine currently affecting large areas of Africa, Eurasia and the Caribbean. ASFV has a complex structure harboring a large dsDNA genome which encodes for more than 160 proteins. One of the proteins, E66L, has recently been involved in arresting gene transcription in the infected host cell. Here, we investigate the role of E66L in the processes of virus replication in swine macrophages and disease production in domestic swine. A recombinant ASFV was developed (ASFV-G-∆E66L), from the virulent parental Georgia 2010 isolate (ASFV-G), harboring the deletion of the E66L gene as a tool to assess the role of the gene. ASFV-G-∆E66L showed that the E66L gene is non-essential for ASFV replication in primary swine macrophages when compared with the parental highly virulent field isolate ASFV-G. Additionally, domestic pigs infected with ASFV-G-∆E66L developed a clinical disease undistinguishable from that produced by ASFV-G. Therefore, E66L is not involved in virus replication or virulence in domestic pigs. |
| first_indexed | 2024-03-11T08:00:58Z |
| format | Article |
| id | doaj.art-a400939203e640de8fce5351705c1b1a |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T08:00:58Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-a400939203e640de8fce5351705c1b1a2023-11-16T23:50:44ZengMDPI AGViruses1999-49152023-02-0115256610.3390/v15020566Evaluation of the Function of ASFV Gene E66L in the Process of Virus Replication and Virulence in SwineElizabeth Ramirez-Medina0Elizabeth A. Vuono1Ayushi Rai2Nallely Espinoza3Alyssa Valladares4Edward Spinard5Lauro Velazquez-Salinas6Douglas P. Gladue7Manuel V. Borca8Plum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, NY 11944, USAPlum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, NY 11944, USAPlum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, NY 11944, USAPlum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, NY 11944, USAPlum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, NY 11944, USAPlum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, NY 11944, USAPlum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, NY 11944, USAPlum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, NY 11944, USAPlum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, NY 11944, USAAfrican swine fever virus (ASFV) is the etiological agent of an economically important disease of swine currently affecting large areas of Africa, Eurasia and the Caribbean. ASFV has a complex structure harboring a large dsDNA genome which encodes for more than 160 proteins. One of the proteins, E66L, has recently been involved in arresting gene transcription in the infected host cell. Here, we investigate the role of E66L in the processes of virus replication in swine macrophages and disease production in domestic swine. A recombinant ASFV was developed (ASFV-G-∆E66L), from the virulent parental Georgia 2010 isolate (ASFV-G), harboring the deletion of the E66L gene as a tool to assess the role of the gene. ASFV-G-∆E66L showed that the E66L gene is non-essential for ASFV replication in primary swine macrophages when compared with the parental highly virulent field isolate ASFV-G. Additionally, domestic pigs infected with ASFV-G-∆E66L developed a clinical disease undistinguishable from that produced by ASFV-G. Therefore, E66L is not involved in virus replication or virulence in domestic pigs.https://www.mdpi.com/1999-4915/15/2/566ASFVASFAfrican swine fever virusE66L |
| spellingShingle | Elizabeth Ramirez-Medina Elizabeth A. Vuono Ayushi Rai Nallely Espinoza Alyssa Valladares Edward Spinard Lauro Velazquez-Salinas Douglas P. Gladue Manuel V. Borca Evaluation of the Function of ASFV Gene E66L in the Process of Virus Replication and Virulence in Swine Viruses ASFV ASF African swine fever virus E66L |
| title | Evaluation of the Function of ASFV Gene E66L in the Process of Virus Replication and Virulence in Swine |
| title_full | Evaluation of the Function of ASFV Gene E66L in the Process of Virus Replication and Virulence in Swine |
| title_fullStr | Evaluation of the Function of ASFV Gene E66L in the Process of Virus Replication and Virulence in Swine |
| title_full_unstemmed | Evaluation of the Function of ASFV Gene E66L in the Process of Virus Replication and Virulence in Swine |
| title_short | Evaluation of the Function of ASFV Gene E66L in the Process of Virus Replication and Virulence in Swine |
| title_sort | evaluation of the function of asfv gene e66l in the process of virus replication and virulence in swine |
| topic | ASFV ASF African swine fever virus E66L |
| url | https://www.mdpi.com/1999-4915/15/2/566 |
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