Distribution of Glycoconjugates terminal sugars during neurohypophysis development in Rat
Background and Objective: The neurohypophysis originates from the floor of diencephalon. Its development controles by several cellular interactions that mediated by some molecules such as cell surface and extra cellular matrix Glycoconjugates terminal sugars. In this study we used lectin histochemic...
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| Format: | Article |
| Language: | fas |
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Golestan University of Medical Sciences
2011-09-01
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| Series: | مجله دانشگاه علوم پزشکی گرگان |
| Subjects: | |
| Online Access: | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-525&slc_lang=en&sid=1 |
| _version_ | 1818835602041208832 |
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| author | Ebrahimzadeh Bideskan AR (PhD) Nikravesh MR (PhD) Fazel AR (PhD) |
| author_facet | Ebrahimzadeh Bideskan AR (PhD) Nikravesh MR (PhD) Fazel AR (PhD) |
| author_sort | Ebrahimzadeh Bideskan AR (PhD) |
| collection | DOAJ |
| description | Background and Objective: The neurohypophysis originates from the floor of diencephalon. Its development controles by several cellular interactions that mediated by some molecules such as cell surface and extra cellular matrix Glycoconjugates terminal sugars. In this study we used lectin histochemichal technique to evaluate distribution of the Glycoconjugates and their changes during development of neurohypophysis. Materials and Methods: This experimental study carried on 40 female and 20 male adult Rats. After mating and appointment day zero of pregnancy, pregnant Rats were sacrificed from days 10-20 of gestation and their embryos were collected for histochemical study. The serial section of head specimens were fixed and incubated with different HRP-lectins from Orange fungus (OFA) Vicica villosa (VVA), Glycine max (SBA), Wistaria floribunda (WFA), peanut (PNA), Griffonia simplicfolia (GSA1-B4), Lotus tetragonolobus (LTA) and Ulex Europeus (UEA-1). OFA, LTA and UEA-1 lectins are specific for terminal sugars α-L–Fucose and WFA, SBA, VVA and PNA are specific for D-GalNAc, α, ß-D-GalNAc and GalNAc, D-Gal-(ß-1-3)- D-GalNAc of complex glycoconjugates respectively. Results: Our findings demonstrated that the reaction of neurohypophysis cells with OFA initiated from gestational GD10 and increased to GD15 (P<0.05) and then increased to GD17 (P<0.05). A few cells of neurohypophysis reacted with PNA from GD13 to GD16 and decreased afterward (P<0.05). Some cells of neurohypophysis reacted with SBA from GD14 to GD18 and decreased afterward (P<0.05). Reacting of many cells of neurohypophysis with WFA started on GD13 and increased to GD15 (P<0.05) and then decreased afterward (P<0.05). Neurohypophysis cells showed no reaction with the UEA-1, LTA, VVA and GSA1-B4 lectins. Conclusion: The expression of Glycoconjugates with terminal sugars α-L–Fucose, α, ß-D-GalNAc and D- Gal– (ß-1-3)- D-GalNAc have importanct role and special spatiotemporal situation in neurohypophysis development. |
| first_indexed | 2024-12-19T02:53:19Z |
| format | Article |
| id | doaj.art-a401ad61cd8b4292990fd45ca4729265 |
| institution | Directory Open Access Journal |
| issn | 1562-4765 2008-4080 |
| language | fas |
| last_indexed | 2024-12-19T02:53:19Z |
| publishDate | 2011-09-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی گرگان |
| spelling | doaj.art-a401ad61cd8b4292990fd45ca47292652022-12-21T20:38:29ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802011-09-011332634Distribution of Glycoconjugates terminal sugars during neurohypophysis development in RatEbrahimzadeh Bideskan AR (PhD)0Nikravesh MR (PhD)1Fazel AR (PhD)2 Assistant Professor, Department of Anatomy, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Professor, Department of Anatomy, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Professor, Department of Anatomy, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Background and Objective: The neurohypophysis originates from the floor of diencephalon. Its development controles by several cellular interactions that mediated by some molecules such as cell surface and extra cellular matrix Glycoconjugates terminal sugars. In this study we used lectin histochemichal technique to evaluate distribution of the Glycoconjugates and their changes during development of neurohypophysis. Materials and Methods: This experimental study carried on 40 female and 20 male adult Rats. After mating and appointment day zero of pregnancy, pregnant Rats were sacrificed from days 10-20 of gestation and their embryos were collected for histochemical study. The serial section of head specimens were fixed and incubated with different HRP-lectins from Orange fungus (OFA) Vicica villosa (VVA), Glycine max (SBA), Wistaria floribunda (WFA), peanut (PNA), Griffonia simplicfolia (GSA1-B4), Lotus tetragonolobus (LTA) and Ulex Europeus (UEA-1). OFA, LTA and UEA-1 lectins are specific for terminal sugars α-L–Fucose and WFA, SBA, VVA and PNA are specific for D-GalNAc, α, ß-D-GalNAc and GalNAc, D-Gal-(ß-1-3)- D-GalNAc of complex glycoconjugates respectively. Results: Our findings demonstrated that the reaction of neurohypophysis cells with OFA initiated from gestational GD10 and increased to GD15 (P<0.05) and then increased to GD17 (P<0.05). A few cells of neurohypophysis reacted with PNA from GD13 to GD16 and decreased afterward (P<0.05). Some cells of neurohypophysis reacted with SBA from GD14 to GD18 and decreased afterward (P<0.05). Reacting of many cells of neurohypophysis with WFA started on GD13 and increased to GD15 (P<0.05) and then decreased afterward (P<0.05). Neurohypophysis cells showed no reaction with the UEA-1, LTA, VVA and GSA1-B4 lectins. Conclusion: The expression of Glycoconjugates with terminal sugars α-L–Fucose, α, ß-D-GalNAc and D- Gal– (ß-1-3)- D-GalNAc have importanct role and special spatiotemporal situation in neurohypophysis development.http://goums.ac.ir/journal/browse.php?a_code=A-10-1-525&slc_lang=en&sid=1NeurohypophysisDevelopmentGlycoconjugatesLectinRat |
| spellingShingle | Ebrahimzadeh Bideskan AR (PhD) Nikravesh MR (PhD) Fazel AR (PhD) Distribution of Glycoconjugates terminal sugars during neurohypophysis development in Rat مجله دانشگاه علوم پزشکی گرگان Neurohypophysis Development Glycoconjugates Lectin Rat |
| title | Distribution of Glycoconjugates terminal sugars during neurohypophysis development in Rat |
| title_full | Distribution of Glycoconjugates terminal sugars during neurohypophysis development in Rat |
| title_fullStr | Distribution of Glycoconjugates terminal sugars during neurohypophysis development in Rat |
| title_full_unstemmed | Distribution of Glycoconjugates terminal sugars during neurohypophysis development in Rat |
| title_short | Distribution of Glycoconjugates terminal sugars during neurohypophysis development in Rat |
| title_sort | distribution of glycoconjugates terminal sugars during neurohypophysis development in rat |
| topic | Neurohypophysis Development Glycoconjugates Lectin Rat |
| url | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-525&slc_lang=en&sid=1 |
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