Evaluation of the effects of the recommended oral dose of diflubenzuron on bovine sperm and oocyte quality using CASA and OPU-IVEP
IntroductionThe aim of this study was to evaluate potential effects of diflubenzuron on the production and quality of gametes, and on in vitro embryo production (IVEP) outcomes, in cattle.MethodsTwo experiments were performed, the first to evaluate effects on semen, and the second on cumulus-oocyte...
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1215722/full |
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| author | Marcelo Cunha Xavier Marcelo Cunha Xavier Leticia Prates Martins Rodrigo Martins Moura Divino Fabio Morais Joao Vitor Lima Barbosa Ricardo Alamino Figueiredo Mauricio Antonio Silva Peixer Rosangela Vieira de Andrade Joao Henrique Moreira Viana Joao Henrique Moreira Viana |
| author_facet | Marcelo Cunha Xavier Marcelo Cunha Xavier Leticia Prates Martins Rodrigo Martins Moura Divino Fabio Morais Joao Vitor Lima Barbosa Ricardo Alamino Figueiredo Mauricio Antonio Silva Peixer Rosangela Vieira de Andrade Joao Henrique Moreira Viana Joao Henrique Moreira Viana |
| author_sort | Marcelo Cunha Xavier |
| collection | DOAJ |
| description | IntroductionThe aim of this study was to evaluate potential effects of diflubenzuron on the production and quality of gametes, and on in vitro embryo production (IVEP) outcomes, in cattle.MethodsTwo experiments were performed, the first to evaluate effects on semen, and the second on cumulus-oocyte complexes (COC) and on IVEP. Nelore (Bos taurus indicus) bulls (n = 14) or heifers (n = 16) were allocated into control (CG) or treatment (DIF) groups. All groups received a mineral mix supplement added (DIF) or not (CG) with diflubenzuron (30 mg/head/day), during 8 weeks. Animals were weighed and blood samples were collected throughout the experimental period. Every other week, bulls were subjected to semen collection and heifers to transvaginal ultrasound-guided follicle aspiration sessions. Semen underwent physical and morphological evaluation, and samples were stored for further computer-assisted sperm analysis. The COC recovered were evaluated according to morphology and those classified as viable were sent to an IVEP laboratory.ResultsDiflubenzuron had no effect (P > 0.05) on average body weight or in any blood hematological or biochemical endpoints, regardless of gender. In experiment 1, there was no difference (P > 0.05) between DIF and CG groups for sperm concentration, morphology, or kinetics. In experiment 2, there was also no effect of diflubenzuron on the number of total, viable, or grade I oocytes, as well as on cleavage or blastocyst rates (P > 0.05).DiscussionIn summary, the oral administration of diflubenzuron, within the recommended dose, has no short-term negative effects on sperm production and quality or on oocyte yield and developmental potential in vitro, in cattle. |
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| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-13T00:25:24Z |
| publishDate | 2023-07-01 |
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| spelling | doaj.art-a40309f3d9d54703b0c9dbeae43a32f92023-07-11T06:44:02ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-07-011010.3389/fvets.2023.12157221215722Evaluation of the effects of the recommended oral dose of diflubenzuron on bovine sperm and oocyte quality using CASA and OPU-IVEPMarcelo Cunha Xavier0Marcelo Cunha Xavier1Leticia Prates Martins2Rodrigo Martins Moura3Divino Fabio Morais4Joao Vitor Lima Barbosa5Ricardo Alamino Figueiredo6Mauricio Antonio Silva Peixer7Rosangela Vieira de Andrade8Joao Henrique Moreira Viana9Joao Henrique Moreira Viana10Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, DF, BrazilBio Biotecnologia da Reprodução Animal, Brasília, DF, BrazilPrograma de Pós-Graduação em Biologia Animal, Universidade de Brasília, Brasília, DF, BrazilPrograma de Pós-Graduação em Biologia Animal, Universidade de Brasília, Brasília, DF, BrazilDepartamento de Medicina Veterinária, Centro Universitário de Brasília, Brasília, DF, BrazilDepartamento de Medicina Veterinária, Centro Universitário de Brasília, Brasília, DF, BrazilEmbrapa Recursos Genéticos e Biotecnologia, Brasília, DF, BrazilBio Biotecnologia da Reprodução Animal, Brasília, DF, BrazilPrograma de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, DF, BrazilPrograma de Pós-Graduação em Biologia Animal, Universidade de Brasília, Brasília, DF, BrazilEmbrapa Recursos Genéticos e Biotecnologia, Brasília, DF, BrazilIntroductionThe aim of this study was to evaluate potential effects of diflubenzuron on the production and quality of gametes, and on in vitro embryo production (IVEP) outcomes, in cattle.MethodsTwo experiments were performed, the first to evaluate effects on semen, and the second on cumulus-oocyte complexes (COC) and on IVEP. Nelore (Bos taurus indicus) bulls (n = 14) or heifers (n = 16) were allocated into control (CG) or treatment (DIF) groups. All groups received a mineral mix supplement added (DIF) or not (CG) with diflubenzuron (30 mg/head/day), during 8 weeks. Animals were weighed and blood samples were collected throughout the experimental period. Every other week, bulls were subjected to semen collection and heifers to transvaginal ultrasound-guided follicle aspiration sessions. Semen underwent physical and morphological evaluation, and samples were stored for further computer-assisted sperm analysis. The COC recovered were evaluated according to morphology and those classified as viable were sent to an IVEP laboratory.ResultsDiflubenzuron had no effect (P > 0.05) on average body weight or in any blood hematological or biochemical endpoints, regardless of gender. In experiment 1, there was no difference (P > 0.05) between DIF and CG groups for sperm concentration, morphology, or kinetics. In experiment 2, there was also no effect of diflubenzuron on the number of total, viable, or grade I oocytes, as well as on cleavage or blastocyst rates (P > 0.05).DiscussionIn summary, the oral administration of diflubenzuron, within the recommended dose, has no short-term negative effects on sperm production and quality or on oocyte yield and developmental potential in vitro, in cattle.https://www.frontiersin.org/articles/10.3389/fvets.2023.1215722/fullDiflyreproductive disruptorsin vitro embryo productionsemenNelore breed |
| spellingShingle | Marcelo Cunha Xavier Marcelo Cunha Xavier Leticia Prates Martins Rodrigo Martins Moura Divino Fabio Morais Joao Vitor Lima Barbosa Ricardo Alamino Figueiredo Mauricio Antonio Silva Peixer Rosangela Vieira de Andrade Joao Henrique Moreira Viana Joao Henrique Moreira Viana Evaluation of the effects of the recommended oral dose of diflubenzuron on bovine sperm and oocyte quality using CASA and OPU-IVEP Frontiers in Veterinary Science Difly reproductive disruptors in vitro embryo production semen Nelore breed |
| title | Evaluation of the effects of the recommended oral dose of diflubenzuron on bovine sperm and oocyte quality using CASA and OPU-IVEP |
| title_full | Evaluation of the effects of the recommended oral dose of diflubenzuron on bovine sperm and oocyte quality using CASA and OPU-IVEP |
| title_fullStr | Evaluation of the effects of the recommended oral dose of diflubenzuron on bovine sperm and oocyte quality using CASA and OPU-IVEP |
| title_full_unstemmed | Evaluation of the effects of the recommended oral dose of diflubenzuron on bovine sperm and oocyte quality using CASA and OPU-IVEP |
| title_short | Evaluation of the effects of the recommended oral dose of diflubenzuron on bovine sperm and oocyte quality using CASA and OPU-IVEP |
| title_sort | evaluation of the effects of the recommended oral dose of diflubenzuron on bovine sperm and oocyte quality using casa and opu ivep |
| topic | Difly reproductive disruptors in vitro embryo production semen Nelore breed |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1215722/full |
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