Fecal bacteria-free filtrate transplantation is proved as an effective way for the recovery of radiation-induced individuals in mice
BackgroundIonizing radiation can cause intestinal microecological dysbiosis, resulting in changes in the composition and function of gut microbiota. Altered gut microbiota is closely related to the development and progression of radiation-induced intestinal damage. Although microbiota-oriented thera...
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| Format: | Article |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1343752/full |
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| author | Hang Zhang Miaomiao Dong Jixia Zheng Yapeng Yang Jinhui He Tianhao Liu Hong Wei |
| author_facet | Hang Zhang Miaomiao Dong Jixia Zheng Yapeng Yang Jinhui He Tianhao Liu Hong Wei |
| author_sort | Hang Zhang |
| collection | DOAJ |
| description | BackgroundIonizing radiation can cause intestinal microecological dysbiosis, resulting in changes in the composition and function of gut microbiota. Altered gut microbiota is closely related to the development and progression of radiation-induced intestinal damage. Although microbiota-oriented therapeutic options such as fecal microbiota transplantation (FMT) have shown some efficacy in treating radiation toxicity, safety concerns endure. Therefore, fecal bacteria-free filtrate transplantation (FFT), which has the potential to become a possible alternative therapy, is well worth investigating. Herein, we performed FFT in a mouse model of radiation exposure and monitored its effects on radiation damage phenotypes, gut microbiota, and metabolomic profiles to assess the effectiveness of FFT as an alternative therapy to FMT safety concerns.ResultsFFT treatment conferred radioprotection against radiation-induced toxicity, representing as better intestinal integrity, robust proinflammatory and anti-inflammatory cytokines homeostasis, and accompanied by significant shifts in gut microbiome. The bacterial compartment of recipients following FFT was characterized by an enrichment of radioprotective microorganisms (members of family Lachnospiraceae). Furthermore, metabolome data revealed increased levels of microbially generated short-chain fatty acids (SCFAs) in the feces of FFT mice.ConclusionsFFT improves radiation-induced intestinal microecological dysbiosis by reshaping intestinal mucosal barrier function, gut microbiota configurations, and host metabolic profiles, highlighting FFT regimen as a promising safe alternative therapy for FMT is effective in the treatment of radiation intestinal injury. |
| first_indexed | 2024-03-08T09:30:12Z |
| format | Article |
| id | doaj.art-a403321010a14ce4ab35b34191769a03 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-08T09:30:12Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-a403321010a14ce4ab35b34191769a032024-01-31T04:40:54ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882024-01-011310.3389/fcimb.2023.13437521343752Fecal bacteria-free filtrate transplantation is proved as an effective way for the recovery of radiation-induced individuals in miceHang Zhang0Miaomiao Dong1Jixia Zheng2Yapeng Yang3Jinhui He4Tianhao Liu5Hong Wei6State Key Laboratory of Agricultural Microbiology, College of Animal Science and Technology, College of Animal Medicine, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Animal Science and Technology, College of Animal Medicine, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Animal Science and Technology, College of Animal Medicine, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Animal Science and Technology, College of Animal Medicine, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Animal Science and Technology, College of Animal Medicine, Huazhong Agricultural University, Wuhan, ChinaDepartment of Gastroenterology, Affiliated Hospital of Jiangnan University, Wuxi, ChinaState Key Laboratory of Agricultural Microbiology, College of Animal Science and Technology, College of Animal Medicine, Huazhong Agricultural University, Wuhan, ChinaBackgroundIonizing radiation can cause intestinal microecological dysbiosis, resulting in changes in the composition and function of gut microbiota. Altered gut microbiota is closely related to the development and progression of radiation-induced intestinal damage. Although microbiota-oriented therapeutic options such as fecal microbiota transplantation (FMT) have shown some efficacy in treating radiation toxicity, safety concerns endure. Therefore, fecal bacteria-free filtrate transplantation (FFT), which has the potential to become a possible alternative therapy, is well worth investigating. Herein, we performed FFT in a mouse model of radiation exposure and monitored its effects on radiation damage phenotypes, gut microbiota, and metabolomic profiles to assess the effectiveness of FFT as an alternative therapy to FMT safety concerns.ResultsFFT treatment conferred radioprotection against radiation-induced toxicity, representing as better intestinal integrity, robust proinflammatory and anti-inflammatory cytokines homeostasis, and accompanied by significant shifts in gut microbiome. The bacterial compartment of recipients following FFT was characterized by an enrichment of radioprotective microorganisms (members of family Lachnospiraceae). Furthermore, metabolome data revealed increased levels of microbially generated short-chain fatty acids (SCFAs) in the feces of FFT mice.ConclusionsFFT improves radiation-induced intestinal microecological dysbiosis by reshaping intestinal mucosal barrier function, gut microbiota configurations, and host metabolic profiles, highlighting FFT regimen as a promising safe alternative therapy for FMT is effective in the treatment of radiation intestinal injury.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1343752/fullfecal bacteria-free filtrate transplantationintestinal mucosal barriergut microbiotaradiation damagemetabolism |
| spellingShingle | Hang Zhang Miaomiao Dong Jixia Zheng Yapeng Yang Jinhui He Tianhao Liu Hong Wei Fecal bacteria-free filtrate transplantation is proved as an effective way for the recovery of radiation-induced individuals in mice Frontiers in Cellular and Infection Microbiology fecal bacteria-free filtrate transplantation intestinal mucosal barrier gut microbiota radiation damage metabolism |
| title | Fecal bacteria-free filtrate transplantation is proved as an effective way for the recovery of radiation-induced individuals in mice |
| title_full | Fecal bacteria-free filtrate transplantation is proved as an effective way for the recovery of radiation-induced individuals in mice |
| title_fullStr | Fecal bacteria-free filtrate transplantation is proved as an effective way for the recovery of radiation-induced individuals in mice |
| title_full_unstemmed | Fecal bacteria-free filtrate transplantation is proved as an effective way for the recovery of radiation-induced individuals in mice |
| title_short | Fecal bacteria-free filtrate transplantation is proved as an effective way for the recovery of radiation-induced individuals in mice |
| title_sort | fecal bacteria free filtrate transplantation is proved as an effective way for the recovery of radiation induced individuals in mice |
| topic | fecal bacteria-free filtrate transplantation intestinal mucosal barrier gut microbiota radiation damage metabolism |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1343752/full |
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