Effects of chromosomal translocation characteristics on fertilization and blastocyst development — a retrospective cohort study
Abstract Objective To determine the effect of different translocation characteristics on fertilization rate and blastocyst development in chromosomal translocation patients. Methods This retrospective cohort study was conducted at the Third Affiliated Hospital of Zhengzhou University From January 20...
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BMC
2023-11-01
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| Series: | BMC Medical Genomics |
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| Online Access: | https://doi.org/10.1186/s12920-023-01715-4 |
| _version_ | 1797636632097062912 |
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| author | Shanshan Wu Jianrui Zhang Yichun Guan Bingnan Ren Yuchao Zhang Xinmi Liu Kexin Wang Mingmei Zhang Zhen Li |
| author_facet | Shanshan Wu Jianrui Zhang Yichun Guan Bingnan Ren Yuchao Zhang Xinmi Liu Kexin Wang Mingmei Zhang Zhen Li |
| author_sort | Shanshan Wu |
| collection | DOAJ |
| description | Abstract Objective To determine the effect of different translocation characteristics on fertilization rate and blastocyst development in chromosomal translocation patients. Methods This retrospective cohort study was conducted at the Third Affiliated Hospital of Zhengzhou University From January 2017 to December 2022.All couples were diagnosed as reciprocal translocation or Robertsonian translocation by karyotype of peripheral blood lymphocytes test. After adjusting for confounding factors, the effect of chromosomal rearrangement characteristics, such as carrier sex, translocation type, chromosome length and break sites, on fertilization rate and embryo development were analysed separately using multiple linear regression. Results In cases of Robertsonian translocation (RobT), the carrier sex plays an independent role in fertilization rate, and the male carriers was lower than that of female carriers (76.16% vs.86.26%, P = 0.009). In reciprocal translocation (RecT), the carrier sex, chromosome types and break sites had no influence on fertilization rate, blastocyst formation rate (P > 0.05). However, patients with human longer chromosomal (chromosomes 1–5) translocation have a lower available blastocyst formation rate (Group AB vs. Group CD: 41.49%vs.46.01%, P = 0.027). For male carriers, the translocation types was an independent factor affecting the fertilization rate, and the RobT was the negative one (B = − 0.075, P = 0 0.009). In female carriers, we did not observe this difference (P = 0.227). Conclusions In patients with chromosomal translocation, the fertilization rate may be influenced by carrier sex and translocation type, chromosomes 1–5 translocation may adversely affect the formation of available blastocysts. Break sites have no role in fertilization and blastocyst development. |
| first_indexed | 2024-03-11T12:37:52Z |
| format | Article |
| id | doaj.art-a40469fa431f4002a830cb905703a188 |
| institution | Directory Open Access Journal |
| issn | 1755-8794 |
| language | English |
| last_indexed | 2024-03-11T12:37:52Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Medical Genomics |
| spelling | doaj.art-a40469fa431f4002a830cb905703a1882023-11-05T12:31:49ZengBMCBMC Medical Genomics1755-87942023-11-0116111010.1186/s12920-023-01715-4Effects of chromosomal translocation characteristics on fertilization and blastocyst development — a retrospective cohort studyShanshan Wu0Jianrui Zhang1Yichun Guan2Bingnan Ren3Yuchao Zhang4Xinmi Liu5Kexin Wang6Mingmei Zhang7Zhen Li8Reproductive Medicine Center, Third Affiliated Hospital of Zhengzhou UniversityReproductive Medicine Center, Third Affiliated Hospital of Zhengzhou UniversityReproductive Medicine Center, Third Affiliated Hospital of Zhengzhou UniversityReproductive Medicine Center, Third Affiliated Hospital of Zhengzhou UniversityReproductive Medicine Center, Third Affiliated Hospital of Zhengzhou UniversityReproductive Medicine Center, Third Affiliated Hospital of Zhengzhou UniversityReproductive Medicine Center, Third Affiliated Hospital of Zhengzhou UniversityReproductive Medicine Center, Third Affiliated Hospital of Zhengzhou UniversityReproductive Medicine Center, Third Affiliated Hospital of Zhengzhou UniversityAbstract Objective To determine the effect of different translocation characteristics on fertilization rate and blastocyst development in chromosomal translocation patients. Methods This retrospective cohort study was conducted at the Third Affiliated Hospital of Zhengzhou University From January 2017 to December 2022.All couples were diagnosed as reciprocal translocation or Robertsonian translocation by karyotype of peripheral blood lymphocytes test. After adjusting for confounding factors, the effect of chromosomal rearrangement characteristics, such as carrier sex, translocation type, chromosome length and break sites, on fertilization rate and embryo development were analysed separately using multiple linear regression. Results In cases of Robertsonian translocation (RobT), the carrier sex plays an independent role in fertilization rate, and the male carriers was lower than that of female carriers (76.16% vs.86.26%, P = 0.009). In reciprocal translocation (RecT), the carrier sex, chromosome types and break sites had no influence on fertilization rate, blastocyst formation rate (P > 0.05). However, patients with human longer chromosomal (chromosomes 1–5) translocation have a lower available blastocyst formation rate (Group AB vs. Group CD: 41.49%vs.46.01%, P = 0.027). For male carriers, the translocation types was an independent factor affecting the fertilization rate, and the RobT was the negative one (B = − 0.075, P = 0 0.009). In female carriers, we did not observe this difference (P = 0.227). Conclusions In patients with chromosomal translocation, the fertilization rate may be influenced by carrier sex and translocation type, chromosomes 1–5 translocation may adversely affect the formation of available blastocysts. Break sites have no role in fertilization and blastocyst development.https://doi.org/10.1186/s12920-023-01715-4Preimplantation genetic testing for chromosomal structural rearrangementsReciprocal translocationRobertsonian translocationFertilizationBlastocyst development |
| spellingShingle | Shanshan Wu Jianrui Zhang Yichun Guan Bingnan Ren Yuchao Zhang Xinmi Liu Kexin Wang Mingmei Zhang Zhen Li Effects of chromosomal translocation characteristics on fertilization and blastocyst development — a retrospective cohort study BMC Medical Genomics Preimplantation genetic testing for chromosomal structural rearrangements Reciprocal translocation Robertsonian translocation Fertilization Blastocyst development |
| title | Effects of chromosomal translocation characteristics on fertilization and blastocyst development — a retrospective cohort study |
| title_full | Effects of chromosomal translocation characteristics on fertilization and blastocyst development — a retrospective cohort study |
| title_fullStr | Effects of chromosomal translocation characteristics on fertilization and blastocyst development — a retrospective cohort study |
| title_full_unstemmed | Effects of chromosomal translocation characteristics on fertilization and blastocyst development — a retrospective cohort study |
| title_short | Effects of chromosomal translocation characteristics on fertilization and blastocyst development — a retrospective cohort study |
| title_sort | effects of chromosomal translocation characteristics on fertilization and blastocyst development a retrospective cohort study |
| topic | Preimplantation genetic testing for chromosomal structural rearrangements Reciprocal translocation Robertsonian translocation Fertilization Blastocyst development |
| url | https://doi.org/10.1186/s12920-023-01715-4 |
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