Isolation of Bacillus thuringiensis from the state of Amazonas, in Brazil, and screening against Aedes aegypti (Diptera, Culicidae)
We investigated the use of Bacillus thuringiensis isolated in the state of Amazonas, in Brazil, for the biological control of the dengue vector Aedes aegypti. From 25 soil samples collected in nine municipalities, 484 bacterial colonies were obtained, 57 (11.78%) of which were identified as B. thuri...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Entomologia
2015-01-01
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| Series: | Revista Brasileira de Entomologia |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0085562615000023 |
| _version_ | 1818137003054596096 |
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| author | Joelma Soares-da-Silva Valéria Cristina Soares Pinheiro Eleilza Litaiff-Abreu Ricardo Antonio Polanczyk Wanderli Pedro Tadei |
| author_facet | Joelma Soares-da-Silva Valéria Cristina Soares Pinheiro Eleilza Litaiff-Abreu Ricardo Antonio Polanczyk Wanderli Pedro Tadei |
| author_sort | Joelma Soares-da-Silva |
| collection | DOAJ |
| description | We investigated the use of Bacillus thuringiensis isolated in the state of Amazonas, in Brazil, for the biological control of the dengue vector Aedes aegypti. From 25 soil samples collected in nine municipalities, 484 bacterial colonies were obtained, 57 (11.78%) of which were identified as B. thuringiensis. Six isolates, IBt-03, IBt-06, IBt-07, IBt-28, IBt-30, and BtAM-27 showed insecticidal activity, and only BtAM-27 presents the five genes investigated cry4Aa, cry4Ba, cry10Aa, cry11Aa, and cry11Ba. The IBt-07 and IBt-28, with lower LC50 values, showed equal toxicity compared to the standards. The isolates of B. thuringiensis from Amazonas constitute potential new means of biological control for A. aegypti, because of their larvicidal activity and the possibility that they may also contain new combinations of toxins. Keywords: Amazonian, Bioinsecticide, Dengue, Vector |
| first_indexed | 2024-12-11T09:49:23Z |
| format | Article |
| id | doaj.art-a4060401622d4c10aedb72d731693325 |
| institution | Directory Open Access Journal |
| issn | 0085-5626 |
| language | English |
| last_indexed | 2024-12-11T09:49:23Z |
| publishDate | 2015-01-01 |
| publisher | Sociedade Brasileira de Entomologia |
| record_format | Article |
| series | Revista Brasileira de Entomologia |
| spelling | doaj.art-a4060401622d4c10aedb72d7316933252022-12-22T01:12:28ZengSociedade Brasileira de EntomologiaRevista Brasileira de Entomologia0085-56262015-01-0159116Isolation of Bacillus thuringiensis from the state of Amazonas, in Brazil, and screening against Aedes aegypti (Diptera, Culicidae)Joelma Soares-da-Silva0Valéria Cristina Soares Pinheiro1Eleilza Litaiff-Abreu2Ricardo Antonio Polanczyk3Wanderli Pedro Tadei4Universidade Federal do Maranhão, Curso Ciências Naturais, Campus VII, Codó, MA, Brazil; Instituto Nacional de Pesquisas da Amazônia, Programa de Pós-Graduação em Entomologia, Laboratório de Malária e Dengue, Manaus, AM, Brazil; Corresponding author.Universidade Estadual do Maranhão, Centro de Estudos Superiores de Caxias, Departamento de Química e Biologia, Laboratório de Entomologia Médica, Caxias, MA, BrazilInstituto Nacional de Pesquisas da Amazônia, Programa de Pós-Graduação em Entomologia, Laboratório de Malária e Dengue, Manaus, AM, BrazilFaculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Departamento Fitossanidade, Jaboticabal, SP, BrazilInstituto Nacional de Pesquisas da Amazônia, Programa de Pós-Graduação em Entomologia, Laboratório de Malária e Dengue, Manaus, AM, BrazilWe investigated the use of Bacillus thuringiensis isolated in the state of Amazonas, in Brazil, for the biological control of the dengue vector Aedes aegypti. From 25 soil samples collected in nine municipalities, 484 bacterial colonies were obtained, 57 (11.78%) of which were identified as B. thuringiensis. Six isolates, IBt-03, IBt-06, IBt-07, IBt-28, IBt-30, and BtAM-27 showed insecticidal activity, and only BtAM-27 presents the five genes investigated cry4Aa, cry4Ba, cry10Aa, cry11Aa, and cry11Ba. The IBt-07 and IBt-28, with lower LC50 values, showed equal toxicity compared to the standards. The isolates of B. thuringiensis from Amazonas constitute potential new means of biological control for A. aegypti, because of their larvicidal activity and the possibility that they may also contain new combinations of toxins. Keywords: Amazonian, Bioinsecticide, Dengue, Vectorhttp://www.sciencedirect.com/science/article/pii/S0085562615000023 |
| spellingShingle | Joelma Soares-da-Silva Valéria Cristina Soares Pinheiro Eleilza Litaiff-Abreu Ricardo Antonio Polanczyk Wanderli Pedro Tadei Isolation of Bacillus thuringiensis from the state of Amazonas, in Brazil, and screening against Aedes aegypti (Diptera, Culicidae) Revista Brasileira de Entomologia |
| title | Isolation of Bacillus thuringiensis from the state of Amazonas, in Brazil, and screening against Aedes aegypti (Diptera, Culicidae) |
| title_full | Isolation of Bacillus thuringiensis from the state of Amazonas, in Brazil, and screening against Aedes aegypti (Diptera, Culicidae) |
| title_fullStr | Isolation of Bacillus thuringiensis from the state of Amazonas, in Brazil, and screening against Aedes aegypti (Diptera, Culicidae) |
| title_full_unstemmed | Isolation of Bacillus thuringiensis from the state of Amazonas, in Brazil, and screening against Aedes aegypti (Diptera, Culicidae) |
| title_short | Isolation of Bacillus thuringiensis from the state of Amazonas, in Brazil, and screening against Aedes aegypti (Diptera, Culicidae) |
| title_sort | isolation of bacillus thuringiensis from the state of amazonas in brazil and screening against aedes aegypti diptera culicidae |
| url | http://www.sciencedirect.com/science/article/pii/S0085562615000023 |
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