Developmental Stage Determines the Accumulation Pattern of UV-Absorbing Compounds in the Model Liverwort <i>Marchantia polymorpha</i> subsp. <i>ruderalis</i> under Controlled Conditions
The liverwort <i>Marchantia polymorpha</i> subsp. <i>ruderalis</i> is an emerging model plant, and some data are available on its responses to ultraviolet (UV) radiation. However, it is unknown if the developmental stage of the thalli modulates the effects of UV radiation on...
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MDPI AG
2021-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/3/473 |
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| author | Gonzalo Soriano María-Ángeles Del-Castillo-Alonso Laura Monforte Rafael Tomás-Las-Heras Javier Martínez-Abaigar Encarnación Núñez-Olivera |
| author_facet | Gonzalo Soriano María-Ángeles Del-Castillo-Alonso Laura Monforte Rafael Tomás-Las-Heras Javier Martínez-Abaigar Encarnación Núñez-Olivera |
| author_sort | Gonzalo Soriano |
| collection | DOAJ |
| description | The liverwort <i>Marchantia polymorpha</i> subsp. <i>ruderalis</i> is an emerging model plant, and some data are available on its responses to ultraviolet (UV) radiation. However, it is unknown if the developmental stage of the thalli modulates the effects of UV radiation on the contents of potentially protecting phenolic compounds. To fill this gap, liverwort samples were exposed or non-exposed to UV radiation for 38 days under controlled conditions, using three developmental stages: gemmae (G), one-month thalli (T1), and two-month thalli (T2). Then, the bulk level of methanol-soluble UV-absorbing compounds and the contents of six flavones (apigenin and luteolin derivatives) were measured. The UV responsiveness decreased with thallus age: G and T1 plants were the most UV-responsive and showed a strong increase in all the variables, with G plants more responsive than T1 plants. In UV-exposed T2 plants, only apigenin derivatives increased and more modestly, probably due to a lower acclimation capacity. Nevertheless, the thalli became progressively tougher due to a decreasing water content, representing a possible structural protection against UV. In UV-exposed plants, the temporal patterns of the accumulation of phenolic compounds were compound-specific. Most compounds decreased with thallus age, but di-glucuronide derivatives showed a bell-shaped pattern, with T1 plants showing the highest contents. A Principal Components Analysis (PCA) ordination of the different samples summarized the results found. The patterns described above should be taken into account to select thalli of an adequate developmental stage for experiments investigating the induction of phenolic compounds by UV radiation. |
| first_indexed | 2024-03-09T05:52:26Z |
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| language | English |
| last_indexed | 2024-03-09T05:52:26Z |
| publishDate | 2021-03-01 |
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| spelling | doaj.art-a407640d477c442e8496428a312980842023-12-03T12:16:33ZengMDPI AGPlants2223-77472021-03-0110347310.3390/plants10030473Developmental Stage Determines the Accumulation Pattern of UV-Absorbing Compounds in the Model Liverwort <i>Marchantia polymorpha</i> subsp. <i>ruderalis</i> under Controlled ConditionsGonzalo Soriano0María-Ángeles Del-Castillo-Alonso1Laura Monforte2Rafael Tomás-Las-Heras3Javier Martínez-Abaigar4Encarnación Núñez-Olivera5Facultad de Ciencia y Tecnología, Universidad de La Rioja, Madre de Dios 53, 26006 Logroño, SpainFacultad de Ciencia y Tecnología, Universidad de La Rioja, Madre de Dios 53, 26006 Logroño, SpainFacultad de Ciencia y Tecnología, Universidad de La Rioja, Madre de Dios 53, 26006 Logroño, SpainFacultad de Ciencia y Tecnología, Universidad de La Rioja, Madre de Dios 53, 26006 Logroño, SpainFacultad de Ciencia y Tecnología, Universidad de La Rioja, Madre de Dios 53, 26006 Logroño, SpainFacultad de Ciencia y Tecnología, Universidad de La Rioja, Madre de Dios 53, 26006 Logroño, SpainThe liverwort <i>Marchantia polymorpha</i> subsp. <i>ruderalis</i> is an emerging model plant, and some data are available on its responses to ultraviolet (UV) radiation. However, it is unknown if the developmental stage of the thalli modulates the effects of UV radiation on the contents of potentially protecting phenolic compounds. To fill this gap, liverwort samples were exposed or non-exposed to UV radiation for 38 days under controlled conditions, using three developmental stages: gemmae (G), one-month thalli (T1), and two-month thalli (T2). Then, the bulk level of methanol-soluble UV-absorbing compounds and the contents of six flavones (apigenin and luteolin derivatives) were measured. The UV responsiveness decreased with thallus age: G and T1 plants were the most UV-responsive and showed a strong increase in all the variables, with G plants more responsive than T1 plants. In UV-exposed T2 plants, only apigenin derivatives increased and more modestly, probably due to a lower acclimation capacity. Nevertheless, the thalli became progressively tougher due to a decreasing water content, representing a possible structural protection against UV. In UV-exposed plants, the temporal patterns of the accumulation of phenolic compounds were compound-specific. Most compounds decreased with thallus age, but di-glucuronide derivatives showed a bell-shaped pattern, with T1 plants showing the highest contents. A Principal Components Analysis (PCA) ordination of the different samples summarized the results found. The patterns described above should be taken into account to select thalli of an adequate developmental stage for experiments investigating the induction of phenolic compounds by UV radiation.https://www.mdpi.com/2223-7747/10/3/473bryophytesliverworts<i>Marchantia polymorpha</i> subsp. <i>ruderalis</i>ultraviolet radiationdevelopmental stagephenolic compounds |
| spellingShingle | Gonzalo Soriano María-Ángeles Del-Castillo-Alonso Laura Monforte Rafael Tomás-Las-Heras Javier Martínez-Abaigar Encarnación Núñez-Olivera Developmental Stage Determines the Accumulation Pattern of UV-Absorbing Compounds in the Model Liverwort <i>Marchantia polymorpha</i> subsp. <i>ruderalis</i> under Controlled Conditions Plants bryophytes liverworts <i>Marchantia polymorpha</i> subsp. <i>ruderalis</i> ultraviolet radiation developmental stage phenolic compounds |
| title | Developmental Stage Determines the Accumulation Pattern of UV-Absorbing Compounds in the Model Liverwort <i>Marchantia polymorpha</i> subsp. <i>ruderalis</i> under Controlled Conditions |
| title_full | Developmental Stage Determines the Accumulation Pattern of UV-Absorbing Compounds in the Model Liverwort <i>Marchantia polymorpha</i> subsp. <i>ruderalis</i> under Controlled Conditions |
| title_fullStr | Developmental Stage Determines the Accumulation Pattern of UV-Absorbing Compounds in the Model Liverwort <i>Marchantia polymorpha</i> subsp. <i>ruderalis</i> under Controlled Conditions |
| title_full_unstemmed | Developmental Stage Determines the Accumulation Pattern of UV-Absorbing Compounds in the Model Liverwort <i>Marchantia polymorpha</i> subsp. <i>ruderalis</i> under Controlled Conditions |
| title_short | Developmental Stage Determines the Accumulation Pattern of UV-Absorbing Compounds in the Model Liverwort <i>Marchantia polymorpha</i> subsp. <i>ruderalis</i> under Controlled Conditions |
| title_sort | developmental stage determines the accumulation pattern of uv absorbing compounds in the model liverwort i marchantia polymorpha i subsp i ruderalis i under controlled conditions |
| topic | bryophytes liverworts <i>Marchantia polymorpha</i> subsp. <i>ruderalis</i> ultraviolet radiation developmental stage phenolic compounds |
| url | https://www.mdpi.com/2223-7747/10/3/473 |
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