Antioxidant capacity and characteristics of theaflavin catechins and ginger freeze-dried extract as affected by extraction techniques

The aim of current research was to determine the effect of different extraction techniques on the antioxidant capacity of green tea, black tea and ginger polyphenols. Initially, the raw materials were subjected to compositional analysis. Afterward, the bioactive moieties from all the samples were ex...

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Main Authors: Ali Imran, Muhammad Umair Arshad, Hafsah Sherwani, Rabia Shabir Ahmad, Muhammad Sajid Arshad, Farhan Saeed, Ghulam Hussain, Muhammad Afzaal, Muhammad Imran, Usman Naeem, Ali Ikram, Faqir M. Anjum
Format: Article
Language:English
Published: Taylor & Francis Group 2021-01-01
Series:International Journal of Food Properties
Subjects:
Online Access:http://dx.doi.org/10.1080/10942912.2021.1953524
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author Ali Imran
Muhammad Umair Arshad
Hafsah Sherwani
Rabia Shabir Ahmad
Muhammad Sajid Arshad
Farhan Saeed
Ghulam Hussain
Muhammad Afzaal
Muhammad Imran
Usman Naeem
Ali Ikram
Faqir M. Anjum
author_facet Ali Imran
Muhammad Umair Arshad
Hafsah Sherwani
Rabia Shabir Ahmad
Muhammad Sajid Arshad
Farhan Saeed
Ghulam Hussain
Muhammad Afzaal
Muhammad Imran
Usman Naeem
Ali Ikram
Faqir M. Anjum
author_sort Ali Imran
collection DOAJ
description The aim of current research was to determine the effect of different extraction techniques on the antioxidant capacity of green tea, black tea and ginger polyphenols. Initially, the raw materials were subjected to compositional analysis. Afterward, the bioactive moieties from all the samples were extracted through ultrasound and conventional solvent extraction method using different solvents (i.e. ethanol, methanol and water). Furthermore, the functional ingredients, namely, catechins and theaflavins were isolated by solvent partition method from green and black tea, respectively. In addition, the extracts and isolates were analyzed for their total phenolic and antioxidant profile using various spectrophotometric assays. For the quantification of theaflavins and catechins, a reverse phase HPLC system was used. Results showed that ultrasound (P > .005) proved more effectual for the extraction and isolation of polyphenols (757.33 mg/100 g GAE) from the tested materials among the other extraction techniques (741.66 mg/100 g GAE). Likewise, green tea showed better performance than the rest and in solvents ethanol performed better as compared to their counter parts. Similarly, the isolates showed higher antioxidant potential as compared to their extracts and order of effectiveness was catechins > theaflavins > Ginger freeze dried extract (GFDE).
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spelling doaj.art-a4076a19809f45eaa82747d9916c621c2022-12-21T17:17:22ZengTaylor & Francis GroupInternational Journal of Food Properties1094-29121532-23862021-01-012411097111610.1080/10942912.2021.19535241953524Antioxidant capacity and characteristics of theaflavin catechins and ginger freeze-dried extract as affected by extraction techniquesAli Imran0Muhammad Umair Arshad1Hafsah Sherwani2Rabia Shabir Ahmad3Muhammad Sajid Arshad4Farhan Saeed5Ghulam Hussain6Muhammad Afzaal7Muhammad Imran8Usman Naeem9Ali Ikram10Faqir M. Anjum11Government College UniversityGovernment College UniversityRHC (Rural Health Centre) MustafabadGovernment College UniversityGovernment College UniversityGovernment College UniversityGovernment College UniversityGovernment College UniversityThe University of LahoreGovernment College UniversityGovernment College UniversityUniversity of the GambiaThe aim of current research was to determine the effect of different extraction techniques on the antioxidant capacity of green tea, black tea and ginger polyphenols. Initially, the raw materials were subjected to compositional analysis. Afterward, the bioactive moieties from all the samples were extracted through ultrasound and conventional solvent extraction method using different solvents (i.e. ethanol, methanol and water). Furthermore, the functional ingredients, namely, catechins and theaflavins were isolated by solvent partition method from green and black tea, respectively. In addition, the extracts and isolates were analyzed for their total phenolic and antioxidant profile using various spectrophotometric assays. For the quantification of theaflavins and catechins, a reverse phase HPLC system was used. Results showed that ultrasound (P > .005) proved more effectual for the extraction and isolation of polyphenols (757.33 mg/100 g GAE) from the tested materials among the other extraction techniques (741.66 mg/100 g GAE). Likewise, green tea showed better performance than the rest and in solvents ethanol performed better as compared to their counter parts. Similarly, the isolates showed higher antioxidant potential as compared to their extracts and order of effectiveness was catechins > theaflavins > Ginger freeze dried extract (GFDE).http://dx.doi.org/10.1080/10942912.2021.1953524green teablack teahplctheaflavinsisolates
spellingShingle Ali Imran
Muhammad Umair Arshad
Hafsah Sherwani
Rabia Shabir Ahmad
Muhammad Sajid Arshad
Farhan Saeed
Ghulam Hussain
Muhammad Afzaal
Muhammad Imran
Usman Naeem
Ali Ikram
Faqir M. Anjum
Antioxidant capacity and characteristics of theaflavin catechins and ginger freeze-dried extract as affected by extraction techniques
International Journal of Food Properties
green tea
black tea
hplc
theaflavins
isolates
title Antioxidant capacity and characteristics of theaflavin catechins and ginger freeze-dried extract as affected by extraction techniques
title_full Antioxidant capacity and characteristics of theaflavin catechins and ginger freeze-dried extract as affected by extraction techniques
title_fullStr Antioxidant capacity and characteristics of theaflavin catechins and ginger freeze-dried extract as affected by extraction techniques
title_full_unstemmed Antioxidant capacity and characteristics of theaflavin catechins and ginger freeze-dried extract as affected by extraction techniques
title_short Antioxidant capacity and characteristics of theaflavin catechins and ginger freeze-dried extract as affected by extraction techniques
title_sort antioxidant capacity and characteristics of theaflavin catechins and ginger freeze dried extract as affected by extraction techniques
topic green tea
black tea
hplc
theaflavins
isolates
url http://dx.doi.org/10.1080/10942912.2021.1953524
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