Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions
To evaluate the antimicrobial agent’s stability stored at −80 °C, six β-lactams (meropenem, ertapenem, imipenem, ceftriaxone, ceftazidime, and piperacillin-tazobactam) were studied using the broth microdilution (BMD). The minimum inhibitory concentration (MIC) r...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/8/4/165 |
| _version_ | 1818893132572393472 |
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| author | Gleice Leite Alexander Lawandi Matthew P. Cheng Todd Lee |
| author_facet | Gleice Leite Alexander Lawandi Matthew P. Cheng Todd Lee |
| author_sort | Gleice Leite |
| collection | DOAJ |
| description | To evaluate the antimicrobial agent’s stability stored at −80 °C, six β-lactams (meropenem, ertapenem, imipenem, ceftriaxone, ceftazidime, and piperacillin-tazobactam) were studied using the broth microdilution (BMD). The minimum inhibitory concentration (MIC) remained accurate with the same amount of frozen drug for at least six months. Thereafter, there was a diminishing drug concentration due to instability. At this temperature, most β-lactams can be frozen as a stock concentration for up to six months without a significant loss in antibiotic activity. |
| first_indexed | 2024-12-19T18:07:44Z |
| format | Article |
| id | doaj.art-a4086e9fa77b4d39b564594ea83ae42b |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-12-19T18:07:44Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-a4086e9fa77b4d39b564594ea83ae42b2022-12-21T20:11:25ZengMDPI AGAntibiotics2079-63822019-09-018416510.3390/antibiotics8040165antibiotics8040165Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth MicrodilutionsGleice Leite0Alexander Lawandi1Matthew P. Cheng2Todd Lee3Research Institute of the McGill University Health Centre, Montréal, QC H4A 3J1, CanadaDivision of Infectious Diseases, Department of Medicine, McGill University Health Centre, Montréal, QC H4A 3J1, CanadaResearch Institute of the McGill University Health Centre, Montréal, QC H4A 3J1, CanadaResearch Institute of the McGill University Health Centre, Montréal, QC H4A 3J1, CanadaTo evaluate the antimicrobial agent’s stability stored at −80 °C, six β-lactams (meropenem, ertapenem, imipenem, ceftriaxone, ceftazidime, and piperacillin-tazobactam) were studied using the broth microdilution (BMD). The minimum inhibitory concentration (MIC) remained accurate with the same amount of frozen drug for at least six months. Thereafter, there was a diminishing drug concentration due to instability. At this temperature, most β-lactams can be frozen as a stock concentration for up to six months without a significant loss in antibiotic activity.https://www.mdpi.com/2079-6382/8/4/165beta-lactamsantimicrobial stabilityfrozen drugsantimicrobial |
| spellingShingle | Gleice Leite Alexander Lawandi Matthew P. Cheng Todd Lee Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions Antibiotics beta-lactams antimicrobial stability frozen drugs antimicrobial |
| title | Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions |
| title_full | Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions |
| title_fullStr | Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions |
| title_full_unstemmed | Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions |
| title_short | Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions |
| title_sort | stability of biological activity of frozen β lactams over time as assessed by time lapsed broth microdilutions |
| topic | beta-lactams antimicrobial stability frozen drugs antimicrobial |
| url | https://www.mdpi.com/2079-6382/8/4/165 |
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