Virulence of Shigatoxigenic and Enteropathogenic <i>Escherichia coli</i> O80:H2 in <i>Galleria mellonella</i> Larvae: Comparison of the Roles of the pS88 Plasmids and STX2d Phage
The invasiveness properties of Shigatoxigenic and enteropathogenic <i>Escherichia coli</i> (STEC and EPEC) O80:H2 in humans and calves are encoded by genes located on a pS88-like ColV conjugative plasmid. The main objectives of this study in larvae of the <i>Galleria mellonella<...
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MDPI AG
2023-06-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/7/420 |
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| author | Rie Ikeda Fanny Laforêt Céline Antoine Mare Adachi Keiji Nakamura Audrey Habets Cassandra Kler Klara De Rauw Tetsuya Hayashi Jacques G. Mainil Damien Thiry |
| author_facet | Rie Ikeda Fanny Laforêt Céline Antoine Mare Adachi Keiji Nakamura Audrey Habets Cassandra Kler Klara De Rauw Tetsuya Hayashi Jacques G. Mainil Damien Thiry |
| author_sort | Rie Ikeda |
| collection | DOAJ |
| description | The invasiveness properties of Shigatoxigenic and enteropathogenic <i>Escherichia coli</i> (STEC and EPEC) O80:H2 in humans and calves are encoded by genes located on a pS88-like ColV conjugative plasmid. The main objectives of this study in larvae of the <i>Galleria mellonella</i> moth were therefore to compare the virulence of eight bovine STEC and EPEC O80:H2, of two <i>E. coli</i> pS88 plasmid transconjugant and STX2d phage transductant K12 DH10B, of four <i>E. coli</i> O80:non-H2, and of the laboratory <i>E. coli</i> K12 DH10B strains. Thirty larvae per strain were inoculated in the last proleg with 10 μL of tenfold dilutions of each bacterial culture corresponding to 10 to 10<sup>6</sup> colony-forming units (CFUs). The larvae were kept at 37 °C and their mortality rate was followed daily for four days. The main results were that: (i) not only the STEC and EPEC O80:H2, but also different <i>E. coli</i> O80:non-H2 were lethal for the larvae at high concentrations (from 10<sup>4</sup> to 10<sup>6</sup> CFU) with some variation according to the strain; (ii) the Stx2d toxin and partially the pS88 plasmid were responsible for the lethality caused by the <i>E. coli</i> O80:H2; (iii) the virulence factors of <i>E. coli</i> O80:non-H2 were not identified. The general conclusions are that, although the <i>Galleria mellonella</i> larvae represent a useful first-line model to study the virulence of bacterial pathogens, they are more limited in identifying their actual virulence properties. |
| first_indexed | 2024-03-11T00:34:26Z |
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| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T00:34:26Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-a40bf5ee064849f3aff2b43bddc4fb822023-11-18T21:42:19ZengMDPI AGVeterinary Sciences2306-73812023-06-0110742010.3390/vetsci10070420Virulence of Shigatoxigenic and Enteropathogenic <i>Escherichia coli</i> O80:H2 in <i>Galleria mellonella</i> Larvae: Comparison of the Roles of the pS88 Plasmids and STX2d PhageRie Ikeda0Fanny Laforêt1Céline Antoine2Mare Adachi3Keiji Nakamura4Audrey Habets5Cassandra Kler6Klara De Rauw7Tetsuya Hayashi8Jacques G. Mainil9Damien Thiry10Veterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, BelgiumVeterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, BelgiumVeterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, BelgiumVeterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, BelgiumDepartment of Bacteriology, Faculty of Medical Science, Kyushu University, Fukuoka 812-8582, JapanVeterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, BelgiumVeterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, BelgiumBelgium National Reference Center of STEC (NRC STEC), Universitair Ziekenhuis Brussel (UZ Brussel), Vrije Universiteit Brussel (VUB), B-1090 Brussels, BelgiumDepartment of Bacteriology, Faculty of Medical Science, Kyushu University, Fukuoka 812-8582, JapanVeterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, BelgiumVeterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, BelgiumThe invasiveness properties of Shigatoxigenic and enteropathogenic <i>Escherichia coli</i> (STEC and EPEC) O80:H2 in humans and calves are encoded by genes located on a pS88-like ColV conjugative plasmid. The main objectives of this study in larvae of the <i>Galleria mellonella</i> moth were therefore to compare the virulence of eight bovine STEC and EPEC O80:H2, of two <i>E. coli</i> pS88 plasmid transconjugant and STX2d phage transductant K12 DH10B, of four <i>E. coli</i> O80:non-H2, and of the laboratory <i>E. coli</i> K12 DH10B strains. Thirty larvae per strain were inoculated in the last proleg with 10 μL of tenfold dilutions of each bacterial culture corresponding to 10 to 10<sup>6</sup> colony-forming units (CFUs). The larvae were kept at 37 °C and their mortality rate was followed daily for four days. The main results were that: (i) not only the STEC and EPEC O80:H2, but also different <i>E. coli</i> O80:non-H2 were lethal for the larvae at high concentrations (from 10<sup>4</sup> to 10<sup>6</sup> CFU) with some variation according to the strain; (ii) the Stx2d toxin and partially the pS88 plasmid were responsible for the lethality caused by the <i>E. coli</i> O80:H2; (iii) the virulence factors of <i>E. coli</i> O80:non-H2 were not identified. The general conclusions are that, although the <i>Galleria mellonella</i> larvae represent a useful first-line model to study the virulence of bacterial pathogens, they are more limited in identifying their actual virulence properties.https://www.mdpi.com/2306-7381/10/7/420<i>Galleria mellonella</i> larvaevirulenceShigatoxigenic <i>Escherichia coli</i>enteropathogenic <i>Escherichia coli</i>O80:H2O80:non-H2 |
| spellingShingle | Rie Ikeda Fanny Laforêt Céline Antoine Mare Adachi Keiji Nakamura Audrey Habets Cassandra Kler Klara De Rauw Tetsuya Hayashi Jacques G. Mainil Damien Thiry Virulence of Shigatoxigenic and Enteropathogenic <i>Escherichia coli</i> O80:H2 in <i>Galleria mellonella</i> Larvae: Comparison of the Roles of the pS88 Plasmids and STX2d Phage Veterinary Sciences <i>Galleria mellonella</i> larvae virulence Shigatoxigenic <i>Escherichia coli</i> enteropathogenic <i>Escherichia coli</i> O80:H2 O80:non-H2 |
| title | Virulence of Shigatoxigenic and Enteropathogenic <i>Escherichia coli</i> O80:H2 in <i>Galleria mellonella</i> Larvae: Comparison of the Roles of the pS88 Plasmids and STX2d Phage |
| title_full | Virulence of Shigatoxigenic and Enteropathogenic <i>Escherichia coli</i> O80:H2 in <i>Galleria mellonella</i> Larvae: Comparison of the Roles of the pS88 Plasmids and STX2d Phage |
| title_fullStr | Virulence of Shigatoxigenic and Enteropathogenic <i>Escherichia coli</i> O80:H2 in <i>Galleria mellonella</i> Larvae: Comparison of the Roles of the pS88 Plasmids and STX2d Phage |
| title_full_unstemmed | Virulence of Shigatoxigenic and Enteropathogenic <i>Escherichia coli</i> O80:H2 in <i>Galleria mellonella</i> Larvae: Comparison of the Roles of the pS88 Plasmids and STX2d Phage |
| title_short | Virulence of Shigatoxigenic and Enteropathogenic <i>Escherichia coli</i> O80:H2 in <i>Galleria mellonella</i> Larvae: Comparison of the Roles of the pS88 Plasmids and STX2d Phage |
| title_sort | virulence of shigatoxigenic and enteropathogenic i escherichia coli i o80 h2 in i galleria mellonella i larvae comparison of the roles of the ps88 plasmids and stx2d phage |
| topic | <i>Galleria mellonella</i> larvae virulence Shigatoxigenic <i>Escherichia coli</i> enteropathogenic <i>Escherichia coli</i> O80:H2 O80:non-H2 |
| url | https://www.mdpi.com/2306-7381/10/7/420 |
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