Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by <i>Escherichia coli</i> Lipopolysaccharide
Graphene oxide (GO), derived from graphene, has remarkable chemical–physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties. The present study aimed to evaluate the anti-inflammatory effects of polypr...
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| Language: | English |
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MDPI AG
2023-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/7/6622 |
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| author | Luigia Fonticoli Francesca Diomede Antonio Nanci Antonella Fontana Ylenia Della Rocca Dainelys Guadarrama Bello Serena Pilato Oriana Trubiani Jacopo Pizzicannella Guya Diletta Marconi |
| author_facet | Luigia Fonticoli Francesca Diomede Antonio Nanci Antonella Fontana Ylenia Della Rocca Dainelys Guadarrama Bello Serena Pilato Oriana Trubiani Jacopo Pizzicannella Guya Diletta Marconi |
| author_sort | Luigia Fonticoli |
| collection | DOAJ |
| description | Graphene oxide (GO), derived from graphene, has remarkable chemical–physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties. The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the <i>Escherichia coli</i> (<i>E. coli</i>) lipopolysaccharide (LPS-E). The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis. Both GO concentrations used in the polypropylene suture threads buttons-GO constructs (PPSTBs-GO) decreased the expression of inflammatory markers in hGFs treated with LPS-E. The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR. Together, these results suggest that enriched PPSTBs-GO modulates the inflammatory process through TLR4/MyD 88/NFκB p65/NLRP3 pathway. |
| first_indexed | 2024-03-11T05:35:07Z |
| format | Article |
| id | doaj.art-a4106e355586491bb4300f893eda129b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T05:35:07Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a4106e355586491bb4300f893eda129b2023-11-17T16:53:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01247662210.3390/ijms24076622Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by <i>Escherichia coli</i> LipopolysaccharideLuigia Fonticoli0Francesca Diomede1Antonio Nanci2Antonella Fontana3Ylenia Della Rocca4Dainelys Guadarrama Bello5Serena Pilato6Oriana Trubiani7Jacopo Pizzicannella8Guya Diletta Marconi9Department of Innovative Technologies in Medicine & Dentistry, University “G. d’Annunzio” Chieti-Pescara, Via dei Vestini, 31, 66100 Chieti, ItalyDepartment of Innovative Technologies in Medicine & Dentistry, University “G. d’Annunzio” Chieti-Pescara, Via dei Vestini, 31, 66100 Chieti, ItalyLaboratory for the Study of Calcified Tissues and Biomaterials, Department of Stomatology, Faculty of Dental Medicine, Université de Montréal, Montreal, QC H3C3J7, CanadaUdA TechLab, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Innovative Technologies in Medicine & Dentistry, University “G. d’Annunzio” Chieti-Pescara, Via dei Vestini, 31, 66100 Chieti, ItalyLaboratory for the Study of Calcified Tissues and Biomaterials, Department of Stomatology, Faculty of Dental Medicine, Université de Montréal, Montreal, QC H3C3J7, CanadaDepartment of Pharmacy, University “G. d’Annunzio” Chieti-Pescara, Via dei Vestini, 31, 66100 Chieti, ItalyDepartment of Innovative Technologies in Medicine & Dentistry, University “G. d’Annunzio” Chieti-Pescara, Via dei Vestini, 31, 66100 Chieti, ItalyUdA TechLab, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Innovative Technologies in Medicine & Dentistry, University “G. d’Annunzio” Chieti-Pescara, Via dei Vestini, 31, 66100 Chieti, ItalyGraphene oxide (GO), derived from graphene, has remarkable chemical–physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties. The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the <i>Escherichia coli</i> (<i>E. coli</i>) lipopolysaccharide (LPS-E). The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis. Both GO concentrations used in the polypropylene suture threads buttons-GO constructs (PPSTBs-GO) decreased the expression of inflammatory markers in hGFs treated with LPS-E. The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR. Together, these results suggest that enriched PPSTBs-GO modulates the inflammatory process through TLR4/MyD 88/NFκB p65/NLRP3 pathway.https://www.mdpi.com/1422-0067/24/7/6622graphene oxideinflammationsuture threadsinflammasomepolypropylene-GO composite |
| spellingShingle | Luigia Fonticoli Francesca Diomede Antonio Nanci Antonella Fontana Ylenia Della Rocca Dainelys Guadarrama Bello Serena Pilato Oriana Trubiani Jacopo Pizzicannella Guya Diletta Marconi Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by <i>Escherichia coli</i> Lipopolysaccharide International Journal of Molecular Sciences graphene oxide inflammation suture threads inflammasome polypropylene-GO composite |
| title | Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by <i>Escherichia coli</i> Lipopolysaccharide |
| title_full | Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by <i>Escherichia coli</i> Lipopolysaccharide |
| title_fullStr | Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by <i>Escherichia coli</i> Lipopolysaccharide |
| title_full_unstemmed | Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by <i>Escherichia coli</i> Lipopolysaccharide |
| title_short | Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by <i>Escherichia coli</i> Lipopolysaccharide |
| title_sort | enriched graphene oxide polypropylene suture threads buttons modulate the inflammatory pathway induced by i escherichia coli i lipopolysaccharide |
| topic | graphene oxide inflammation suture threads inflammasome polypropylene-GO composite |
| url | https://www.mdpi.com/1422-0067/24/7/6622 |
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