Quantification of nematic cell polarity in three-dimensional tissues.
How epithelial cells coordinate their polarity to form functional tissues is an open question in cell biology. Here, we characterize a unique type of polarity found in liver tissue, nematic cell polarity, which is different from vectorial cell polarity in simple, sheet-like epithelia. We propose a c...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-12-01
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| Series: | PLoS Computational Biology |
| Online Access: | https://doi.org/10.1371/journal.pcbi.1008412 |
| _version_ | 1818582903432413184 |
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| author | André Scholich Simon Syga Hernán Morales-Navarrete Fabián Segovia-Miranda Hidenori Nonaka Kirstin Meyer Walter de Back Lutz Brusch Yannis Kalaidzidis Marino Zerial Frank Jülicher Benjamin M Friedrich |
| author_facet | André Scholich Simon Syga Hernán Morales-Navarrete Fabián Segovia-Miranda Hidenori Nonaka Kirstin Meyer Walter de Back Lutz Brusch Yannis Kalaidzidis Marino Zerial Frank Jülicher Benjamin M Friedrich |
| author_sort | André Scholich |
| collection | DOAJ |
| description | How epithelial cells coordinate their polarity to form functional tissues is an open question in cell biology. Here, we characterize a unique type of polarity found in liver tissue, nematic cell polarity, which is different from vectorial cell polarity in simple, sheet-like epithelia. We propose a conceptual and algorithmic framework to characterize complex patterns of polarity proteins on the surface of a cell in terms of a multipole expansion. To rigorously quantify previously observed tissue-level patterns of nematic cell polarity (Morales-Navarrete et al., eLife 2019), we introduce the concept of co-orientational order parameters, which generalize the known biaxial order parameters of the theory of liquid crystals. Applying these concepts to three-dimensional reconstructions of single cells from high-resolution imaging data of mouse liver tissue, we show that the axes of nematic cell polarity of hepatocytes exhibit local coordination and are aligned with the biaxially anisotropic sinusoidal network for blood transport. Our study characterizes liver tissue as a biological example of a biaxial liquid crystal. The general methodology developed here could be applied to other tissues and in-vitro organoids. |
| first_indexed | 2024-12-16T07:56:47Z |
| format | Article |
| id | doaj.art-a411acdfd1eb43caad3cd203f6095013 |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2024-12-16T07:56:47Z |
| publishDate | 2020-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-a411acdfd1eb43caad3cd203f60950132022-12-21T22:38:42ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582020-12-011612e100841210.1371/journal.pcbi.1008412Quantification of nematic cell polarity in three-dimensional tissues.André ScholichSimon SygaHernán Morales-NavarreteFabián Segovia-MirandaHidenori NonakaKirstin MeyerWalter de BackLutz BruschYannis KalaidzidisMarino ZerialFrank JülicherBenjamin M FriedrichHow epithelial cells coordinate their polarity to form functional tissues is an open question in cell biology. Here, we characterize a unique type of polarity found in liver tissue, nematic cell polarity, which is different from vectorial cell polarity in simple, sheet-like epithelia. We propose a conceptual and algorithmic framework to characterize complex patterns of polarity proteins on the surface of a cell in terms of a multipole expansion. To rigorously quantify previously observed tissue-level patterns of nematic cell polarity (Morales-Navarrete et al., eLife 2019), we introduce the concept of co-orientational order parameters, which generalize the known biaxial order parameters of the theory of liquid crystals. Applying these concepts to three-dimensional reconstructions of single cells from high-resolution imaging data of mouse liver tissue, we show that the axes of nematic cell polarity of hepatocytes exhibit local coordination and are aligned with the biaxially anisotropic sinusoidal network for blood transport. Our study characterizes liver tissue as a biological example of a biaxial liquid crystal. The general methodology developed here could be applied to other tissues and in-vitro organoids.https://doi.org/10.1371/journal.pcbi.1008412 |
| spellingShingle | André Scholich Simon Syga Hernán Morales-Navarrete Fabián Segovia-Miranda Hidenori Nonaka Kirstin Meyer Walter de Back Lutz Brusch Yannis Kalaidzidis Marino Zerial Frank Jülicher Benjamin M Friedrich Quantification of nematic cell polarity in three-dimensional tissues. PLoS Computational Biology |
| title | Quantification of nematic cell polarity in three-dimensional tissues. |
| title_full | Quantification of nematic cell polarity in three-dimensional tissues. |
| title_fullStr | Quantification of nematic cell polarity in three-dimensional tissues. |
| title_full_unstemmed | Quantification of nematic cell polarity in three-dimensional tissues. |
| title_short | Quantification of nematic cell polarity in three-dimensional tissues. |
| title_sort | quantification of nematic cell polarity in three dimensional tissues |
| url | https://doi.org/10.1371/journal.pcbi.1008412 |
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