Development and Characterization of Probiotic Beers with <i>Saccharomyces boulardii</i> as an Alternative to Conventional Brewer’s Yeast

The development of new non-dairy probiotic foods is interesting, given lactose intolerance, milk allergies, and the growing trend of vegetarianism. In this paper, beer has been used as a probiotic delivery matrix, using <i>Saccharomyces boulardii</i> as an alternative to conventional bre...

Full description

Bibliographic Details
Main Authors: Ana Belén Díaz, Enrique Durán-Guerrero, Sergio Valiente, Remedios Castro, Cristina Lasanta
Format: Article
Language:English
Published: MDPI AG 2023-07-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/12/15/2912
_version_ 1797586753453817856
author Ana Belén Díaz
Enrique Durán-Guerrero
Sergio Valiente
Remedios Castro
Cristina Lasanta
author_facet Ana Belén Díaz
Enrique Durán-Guerrero
Sergio Valiente
Remedios Castro
Cristina Lasanta
author_sort Ana Belén Díaz
collection DOAJ
description The development of new non-dairy probiotic foods is interesting, given lactose intolerance, milk allergies, and the growing trend of vegetarianism. In this paper, beer has been used as a probiotic delivery matrix, using <i>Saccharomyces boulardii</i> as an alternative to conventional brewer’s yeast. The strain was able to grow in worts prepared with hops containing different alpha-acid concentrations, attaining in all cases a final cell concentration above 1·10<sup>8</sup> cells mL<sup>−1</sup>. Some differences were found in the physicochemical parameters of beers brewed with <i>S. boulardii</i> compared to those brewed with a standard brewer’s yeast. Probiotic beers turned out to be less cloudy, which could help with a possible filtering step; less alcoholic in some cases; a healthier alternative; and with a slightly lower pH, interesting for the reduction of spoilage risk. Thirty volatile compounds were determined in the samples, and, in general, the beers brewed with the probiotic yeast presented significantly higher concentrations for the majority of the studied volatile compounds. In addition, multivariate statistical analysis was successfully performed to differentiate the beers obtained in terms of their volatile composition. Probiotic and standard beers were also subjected to sensory analysis, and they presented similar results in their overall impression.
first_indexed 2024-03-11T00:27:34Z
format Article
id doaj.art-a412219280264a7089d61026c06c5e3c
institution Directory Open Access Journal
issn 2304-8158
language English
last_indexed 2024-03-11T00:27:34Z
publishDate 2023-07-01
publisher MDPI AG
record_format Article
series Foods
spelling doaj.art-a412219280264a7089d61026c06c5e3c2023-11-18T22:54:55ZengMDPI AGFoods2304-81582023-07-011215291210.3390/foods12152912Development and Characterization of Probiotic Beers with <i>Saccharomyces boulardii</i> as an Alternative to Conventional Brewer’s YeastAna Belén Díaz0Enrique Durán-Guerrero1Sergio Valiente2Remedios Castro3Cristina Lasanta4Chemical Engineering and Food Technology Department, Faculty of Sciences-IVAGRO, University of Cadiz, Agrifood Campus of International Excellence (CeiA3), Polígono Río San Pedro, s/n, Puerto Real, 11510 Cadiz, SpainAnalytical Chemistry Department, Faculty of Sciences-IVAGRO, University of Cadiz, Agrifood Campus of International Excellence (CeiA3), Polígono Río San Pedro, s/n, Puerto Real, 11510 Cadiz, SpainChemical Engineering and Food Technology Department, Faculty of Sciences-IVAGRO, University of Cadiz, Agrifood Campus of International Excellence (CeiA3), Polígono Río San Pedro, s/n, Puerto Real, 11510 Cadiz, SpainAnalytical Chemistry Department, Faculty of Sciences-IVAGRO, University of Cadiz, Agrifood Campus of International Excellence (CeiA3), Polígono Río San Pedro, s/n, Puerto Real, 11510 Cadiz, SpainChemical Engineering and Food Technology Department, Faculty of Sciences-IVAGRO, University of Cadiz, Agrifood Campus of International Excellence (CeiA3), Polígono Río San Pedro, s/n, Puerto Real, 11510 Cadiz, SpainThe development of new non-dairy probiotic foods is interesting, given lactose intolerance, milk allergies, and the growing trend of vegetarianism. In this paper, beer has been used as a probiotic delivery matrix, using <i>Saccharomyces boulardii</i> as an alternative to conventional brewer’s yeast. The strain was able to grow in worts prepared with hops containing different alpha-acid concentrations, attaining in all cases a final cell concentration above 1·10<sup>8</sup> cells mL<sup>−1</sup>. Some differences were found in the physicochemical parameters of beers brewed with <i>S. boulardii</i> compared to those brewed with a standard brewer’s yeast. Probiotic beers turned out to be less cloudy, which could help with a possible filtering step; less alcoholic in some cases; a healthier alternative; and with a slightly lower pH, interesting for the reduction of spoilage risk. Thirty volatile compounds were determined in the samples, and, in general, the beers brewed with the probiotic yeast presented significantly higher concentrations for the majority of the studied volatile compounds. In addition, multivariate statistical analysis was successfully performed to differentiate the beers obtained in terms of their volatile composition. Probiotic and standard beers were also subjected to sensory analysis, and they presented similar results in their overall impression.https://www.mdpi.com/2304-8158/12/15/2912probioticbeer<i>Saccharomyces boulardii</i>volatile compoundssensory analysis
spellingShingle Ana Belén Díaz
Enrique Durán-Guerrero
Sergio Valiente
Remedios Castro
Cristina Lasanta
Development and Characterization of Probiotic Beers with <i>Saccharomyces boulardii</i> as an Alternative to Conventional Brewer’s Yeast
Foods
probiotic
beer
<i>Saccharomyces boulardii</i>
volatile compounds
sensory analysis
title Development and Characterization of Probiotic Beers with <i>Saccharomyces boulardii</i> as an Alternative to Conventional Brewer’s Yeast
title_full Development and Characterization of Probiotic Beers with <i>Saccharomyces boulardii</i> as an Alternative to Conventional Brewer’s Yeast
title_fullStr Development and Characterization of Probiotic Beers with <i>Saccharomyces boulardii</i> as an Alternative to Conventional Brewer’s Yeast
title_full_unstemmed Development and Characterization of Probiotic Beers with <i>Saccharomyces boulardii</i> as an Alternative to Conventional Brewer’s Yeast
title_short Development and Characterization of Probiotic Beers with <i>Saccharomyces boulardii</i> as an Alternative to Conventional Brewer’s Yeast
title_sort development and characterization of probiotic beers with i saccharomyces boulardii i as an alternative to conventional brewer s yeast
topic probiotic
beer
<i>Saccharomyces boulardii</i>
volatile compounds
sensory analysis
url https://www.mdpi.com/2304-8158/12/15/2912
work_keys_str_mv AT anabelendiaz developmentandcharacterizationofprobioticbeerswithisaccharomycesboulardiiiasanalternativetoconventionalbrewersyeast
AT enriqueduranguerrero developmentandcharacterizationofprobioticbeerswithisaccharomycesboulardiiiasanalternativetoconventionalbrewersyeast
AT sergiovaliente developmentandcharacterizationofprobioticbeerswithisaccharomycesboulardiiiasanalternativetoconventionalbrewersyeast
AT remedioscastro developmentandcharacterizationofprobioticbeerswithisaccharomycesboulardiiiasanalternativetoconventionalbrewersyeast
AT cristinalasanta developmentandcharacterizationofprobioticbeerswithisaccharomycesboulardiiiasanalternativetoconventionalbrewersyeast