Nanomechanics – The Link to Biology and Chemistry
Biological and chemical processes can be transduced into nanomechanical motion via change of surface stress on a cantilever. By coating the surface of each cantilever of a micro-fabricated array of silicon cantilevers with a different polymer, a versatile vapor sensor is obtained that is a...
| Main Authors: | , , |
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| Format: | Article |
| Language: | deu |
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Swiss Chemical Society
2002-10-01
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| Series: | CHIMIA |
| Subjects: | |
| Online Access: | https://chimia.ch/chimia/article/view/3614 |
| Summary: | Biological and chemical processes can be transduced into nanomechanical motion via change of surface stress on a cantilever. By coating the surface of each cantilever of a micro-fabricated array of silicon cantilevers with a different polymer, a versatile vapor sensor is obtained
that is able to discriminate between various solvent vapors using principal-component analysis techniques. In liquids such sensors allow rapid quantitative and qualitative detection of non-labeled biomolecules. Differential measurements of cantilever deflection (with respect to an unspecific
reference cantilever) allow the detection of sequence-specific DNA hybridization. Single-stranded thiolated DNA 12-mer sequences, anchored onto the surface of the gold-coated cantilevers of the array, provide a biosensor for the detection of their complementary strands in buffer solution.
The influence of the target-molecule concentration on the cantilever deflection is studied, and a value for the thermodynamic surface-solution equilibrium constant is derived from measurements on a cantilever.
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| ISSN: | 0009-4293 2673-2424 |