Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model
Abstract Hyperuricemia (HUA) is a metabolic syndrome caused by abnormal purine metabolism. Although recent studies have noted a relationship between the gut microbiota and gout, whether the microbiota could ameliorate HUA-associated systemic purine metabolism remains unclear. In this study, we const...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-03-01
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| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-024-00486-9 |
| _version_ | 1797247486067212288 |
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| author | Yang Fu Yong-Song Chen Dai-Yang Xia Xiao-Dan Luo Hao-Tong Luo Jie Pan Wei-Qing Ma Jin-Ze Li Qian-Yuan Mo Qiang Tu Meng-Meng Li Yue Zhao Yu Li Yi-Teng Huang Zhi-Xian Chen Zhen-Jun Li Lukuyu Bernard Michel Dione You-Ming Zhang Kai Miao Jian-Ying Chen Shan-Shan Zhu Jie Ren Ling-Juan Zhou Xian-Zhi Jiang Juan Chen Zhen-Ping Lin Jun-Peng Chen Hui Ye Qing-Yun Cao Yong-Wen Zhu Lin Yang Xue Wang Wen-Ce Wang |
| author_facet | Yang Fu Yong-Song Chen Dai-Yang Xia Xiao-Dan Luo Hao-Tong Luo Jie Pan Wei-Qing Ma Jin-Ze Li Qian-Yuan Mo Qiang Tu Meng-Meng Li Yue Zhao Yu Li Yi-Teng Huang Zhi-Xian Chen Zhen-Jun Li Lukuyu Bernard Michel Dione You-Ming Zhang Kai Miao Jian-Ying Chen Shan-Shan Zhu Jie Ren Ling-Juan Zhou Xian-Zhi Jiang Juan Chen Zhen-Ping Lin Jun-Peng Chen Hui Ye Qing-Yun Cao Yong-Wen Zhu Lin Yang Xue Wang Wen-Ce Wang |
| author_sort | Yang Fu |
| collection | DOAJ |
| description | Abstract Hyperuricemia (HUA) is a metabolic syndrome caused by abnormal purine metabolism. Although recent studies have noted a relationship between the gut microbiota and gout, whether the microbiota could ameliorate HUA-associated systemic purine metabolism remains unclear. In this study, we constructed a novel model of HUA in geese and investigated the mechanism by which Lactobacillus rhamnosus GG (LGG) could have beneficial effects on HUA. The administration of antibiotics and fecal microbiota transplantation (FMT) experiments were used in this HUA goose model. The effects of LGG and its metabolites on HUA were evaluated in vivo and in vitro. Heterogeneous expression and gene knockout of LGG revealed the mechanism of LGG. Multi-omics analysis revealed that the Lactobacillus genus is associated with changes in purine metabolism in HUA. This study showed that LGG and its metabolites could alleviate HUA through the gut-liver-kidney axis. Whole-genome analysis, heterogeneous expression, and gene knockout of LGG enzymes ABC-type multidrug transport system (ABCT), inosine-uridine nucleoside N-ribohydrolase (iunH), and xanthine permease (pbuX) demonstrated the function of nucleoside degradation in LGG. Multi-omics and a correlation analysis in HUA patients and this goose model revealed that a serum proline deficiency, as well as changes in Collinsella and Lactobacillus, may be associated with the occurrence of HUA. Our findings demonstrated the potential of a goose model of diet-induced HUA, and LGG and proline could be promising therapies for HUA. |
| first_indexed | 2024-04-24T19:59:27Z |
| format | Article |
| id | doaj.art-a414ed3b40c247e99a28cc885fb903b7 |
| institution | Directory Open Access Journal |
| issn | 2055-5008 |
| language | English |
| last_indexed | 2024-04-24T19:59:27Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj.art-a414ed3b40c247e99a28cc885fb903b72024-03-24T12:12:05ZengNature Portfolionpj Biofilms and Microbiomes2055-50082024-03-0110112210.1038/s41522-024-00486-9Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel modelYang Fu0Yong-Song Chen1Dai-Yang Xia2Xiao-Dan Luo3Hao-Tong Luo4Jie Pan5Wei-Qing Ma6Jin-Ze Li7Qian-Yuan Mo8Qiang Tu9Meng-Meng Li10Yue Zhao11Yu Li12Yi-Teng Huang13Zhi-Xian Chen14Zhen-Jun Li15Lukuyu Bernard16Michel Dione17You-Ming Zhang18Kai Miao19Jian-Ying Chen20Shan-Shan Zhu21Jie Ren22Ling-Juan Zhou23Xian-Zhi Jiang24Juan Chen25Zhen-Ping Lin26Jun-Peng Chen27Hui Ye28Qing-Yun Cao29Yong-Wen Zhu30Lin Yang31Xue Wang32Wen-Ce Wang33State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityClinical Research Center, The First Affiliated Hospital of Shantou University Medical CollegeSchool of Marine Sciences, Sun Yat-sen University, and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai)State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityHunan Shihua Biotech Co. Ltd.State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityState Key Laboratory of Microbial Technology, Shandong UniversitySchool of Agricultural Science and Engineering, Liaocheng UniversityState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityClinical Research Center, The First Affiliated Hospital of Shantou University Medical CollegeClinical Research Center, The First Affiliated Hospital of Shantou University Medical CollegeKey Laboratory of Carcinogenesis and Translational Research, Departments of Lymphoma, Radiology and Nuclear Medicine, Peking University Cancer Hospital and InstituteInternational Livestock Research InstituteInternational Livestock Research InstituteState Key Laboratory of Microbial Technology, Shandong UniversityCancer Center, Faculty of Health Sciences, University of MacauState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityDepartment of Rheumatology and Immunology, The First Affiliated Hospital of Jinan UniversityState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityMicrobiome Research Center, Moon (Guangzhou) Biotech Co. Ltd.Microbiome Research Center, Moon (Guangzhou) Biotech Co. Ltd.Shantou Baisha Research Institute of Origin Species of Poultry and StockShantou Baisha Research Institute of Origin Species of Poultry and StockState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityAbstract Hyperuricemia (HUA) is a metabolic syndrome caused by abnormal purine metabolism. Although recent studies have noted a relationship between the gut microbiota and gout, whether the microbiota could ameliorate HUA-associated systemic purine metabolism remains unclear. In this study, we constructed a novel model of HUA in geese and investigated the mechanism by which Lactobacillus rhamnosus GG (LGG) could have beneficial effects on HUA. The administration of antibiotics and fecal microbiota transplantation (FMT) experiments were used in this HUA goose model. The effects of LGG and its metabolites on HUA were evaluated in vivo and in vitro. Heterogeneous expression and gene knockout of LGG revealed the mechanism of LGG. Multi-omics analysis revealed that the Lactobacillus genus is associated with changes in purine metabolism in HUA. This study showed that LGG and its metabolites could alleviate HUA through the gut-liver-kidney axis. Whole-genome analysis, heterogeneous expression, and gene knockout of LGG enzymes ABC-type multidrug transport system (ABCT), inosine-uridine nucleoside N-ribohydrolase (iunH), and xanthine permease (pbuX) demonstrated the function of nucleoside degradation in LGG. Multi-omics and a correlation analysis in HUA patients and this goose model revealed that a serum proline deficiency, as well as changes in Collinsella and Lactobacillus, may be associated with the occurrence of HUA. Our findings demonstrated the potential of a goose model of diet-induced HUA, and LGG and proline could be promising therapies for HUA.https://doi.org/10.1038/s41522-024-00486-9 |
| spellingShingle | Yang Fu Yong-Song Chen Dai-Yang Xia Xiao-Dan Luo Hao-Tong Luo Jie Pan Wei-Qing Ma Jin-Ze Li Qian-Yuan Mo Qiang Tu Meng-Meng Li Yue Zhao Yu Li Yi-Teng Huang Zhi-Xian Chen Zhen-Jun Li Lukuyu Bernard Michel Dione You-Ming Zhang Kai Miao Jian-Ying Chen Shan-Shan Zhu Jie Ren Ling-Juan Zhou Xian-Zhi Jiang Juan Chen Zhen-Ping Lin Jun-Peng Chen Hui Ye Qing-Yun Cao Yong-Wen Zhu Lin Yang Xue Wang Wen-Ce Wang Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model npj Biofilms and Microbiomes |
| title | Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model |
| title_full | Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model |
| title_fullStr | Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model |
| title_full_unstemmed | Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model |
| title_short | Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model |
| title_sort | lactobacillus rhamnosus gg ameliorates hyperuricemia in a novel model |
| url | https://doi.org/10.1038/s41522-024-00486-9 |
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