Synergic interaction of aromatic compounds anethol and carvone against nematode Haemonchus
The aim of this work was to evaluate the aromatic compounds Anethole and Carvone and its synergic anthelmintic interactions against sheep gastrointestinal nematode Haemonchus contortus multi resistant strain. Anthelmintic resistance has been a hindrance in animal production causing economic losses a...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto de Zootecnia
2015-02-01
|
| Series: | Boletim de Indústria Animal |
| Subjects: | |
| Online Access: | http://ojs.iz.sp.gov.br/index.php/bia/article/view/435 |
| _version_ | 1797749647230369792 |
|---|---|
| author | Luciana Morita Katiki Andiara Moraes Evangelista Barbieri Bruno Ceneviva Fornazari Rafael Canonenco Araújo Helder Louvandini Ricardo Lopes Dias da Costa Mauro Sartori Bueno Cecília José Veríssimo |
| author_facet | Luciana Morita Katiki Andiara Moraes Evangelista Barbieri Bruno Ceneviva Fornazari Rafael Canonenco Araújo Helder Louvandini Ricardo Lopes Dias da Costa Mauro Sartori Bueno Cecília José Veríssimo |
| author_sort | Luciana Morita Katiki |
| collection | DOAJ |
| description | The aim of this work was to evaluate the aromatic compounds Anethole and Carvone and its synergic anthelmintic interactions against sheep gastrointestinal nematode Haemonchus contortus multi resistant strain. Anthelmintic resistance has been a hindrance in animal production causing economic losses and consequence of the repetitive use of those ineffective chemicals it is usual to found contamination on meat, milk and environment. New sources of anthelmintic compounds have been investigated. Plant extracts and natural compounds have demonstrated themselves as promising candidates. Eggs were collected from feces of sheep infected with H.contortus. A hundred eggs were exposed to solutions of Anethole and Carvone and half and half Anethole and Carvone diluted in water and solvent Tween 80 in concentrations ranging from 2.08 mg/mL to 0.007mg/mL (decreasing three fold concentrations) with six replicates using a 24 well plate to perform tests. Eggs were incubated for 24 h at 27°C and the count of eggs hatched was done using an inverted microscope. Data were analyzed by SAS Probit to estimate LC50 with independent variables (dose) transformed by natural logarithm (log dose). The LC50 (fiducial limits) in mg/mL of Anethole was 0.070 (0.068-0.076), Carvone was 0.085 (0.081-0.088) and the synergic interaction of Anethole+ Carvone was 0.013 (0.012-0.014), according to Table 1. Gas chromatography/mass spectrometry was performed for Anethole and Carvone and 98.14% and 99.88% were the similarity with authentic molecules, respectively. |
| first_indexed | 2024-03-12T16:21:42Z |
| format | Article |
| id | doaj.art-a415e04253804552aa9e108405eee52e |
| institution | Directory Open Access Journal |
| issn | 1981-4100 |
| language | English |
| last_indexed | 2024-03-12T16:21:42Z |
| publishDate | 2015-02-01 |
| publisher | Instituto de Zootecnia |
| record_format | Article |
| series | Boletim de Indústria Animal |
| spelling | doaj.art-a415e04253804552aa9e108405eee52e2023-08-08T23:03:17ZengInstituto de ZootecniaBoletim de Indústria Animal1981-41002015-02-0171Synergic interaction of aromatic compounds anethol and carvone against nematode HaemonchusLuciana Morita KatikiAndiara Moraes Evangelista BarbieriBruno Ceneviva FornazariRafael Canonenco AraújoHelder LouvandiniRicardo Lopes Dias da CostaMauro Sartori BuenoCecília José VeríssimoThe aim of this work was to evaluate the aromatic compounds Anethole and Carvone and its synergic anthelmintic interactions against sheep gastrointestinal nematode Haemonchus contortus multi resistant strain. Anthelmintic resistance has been a hindrance in animal production causing economic losses and consequence of the repetitive use of those ineffective chemicals it is usual to found contamination on meat, milk and environment. New sources of anthelmintic compounds have been investigated. Plant extracts and natural compounds have demonstrated themselves as promising candidates. Eggs were collected from feces of sheep infected with H.contortus. A hundred eggs were exposed to solutions of Anethole and Carvone and half and half Anethole and Carvone diluted in water and solvent Tween 80 in concentrations ranging from 2.08 mg/mL to 0.007mg/mL (decreasing three fold concentrations) with six replicates using a 24 well plate to perform tests. Eggs were incubated for 24 h at 27°C and the count of eggs hatched was done using an inverted microscope. Data were analyzed by SAS Probit to estimate LC50 with independent variables (dose) transformed by natural logarithm (log dose). The LC50 (fiducial limits) in mg/mL of Anethole was 0.070 (0.068-0.076), Carvone was 0.085 (0.081-0.088) and the synergic interaction of Anethole+ Carvone was 0.013 (0.012-0.014), according to Table 1. Gas chromatography/mass spectrometry was performed for Anethole and Carvone and 98.14% and 99.88% were the similarity with authentic molecules, respectively.http://ojs.iz.sp.gov.br/index.php/bia/article/view/435essential oilH.contortusin vitro test |
| spellingShingle | Luciana Morita Katiki Andiara Moraes Evangelista Barbieri Bruno Ceneviva Fornazari Rafael Canonenco Araújo Helder Louvandini Ricardo Lopes Dias da Costa Mauro Sartori Bueno Cecília José Veríssimo Synergic interaction of aromatic compounds anethol and carvone against nematode Haemonchus Boletim de Indústria Animal essential oil H.contortus in vitro test |
| title | Synergic interaction of aromatic compounds anethol and carvone against nematode Haemonchus |
| title_full | Synergic interaction of aromatic compounds anethol and carvone against nematode Haemonchus |
| title_fullStr | Synergic interaction of aromatic compounds anethol and carvone against nematode Haemonchus |
| title_full_unstemmed | Synergic interaction of aromatic compounds anethol and carvone against nematode Haemonchus |
| title_short | Synergic interaction of aromatic compounds anethol and carvone against nematode Haemonchus |
| title_sort | synergic interaction of aromatic compounds anethol and carvone against nematode haemonchus |
| topic | essential oil H.contortus in vitro test |
| url | http://ojs.iz.sp.gov.br/index.php/bia/article/view/435 |
| work_keys_str_mv | AT lucianamoritakatiki synergicinteractionofaromaticcompoundsanetholandcarvoneagainstnematodehaemonchus AT andiaramoraesevangelistabarbieri synergicinteractionofaromaticcompoundsanetholandcarvoneagainstnematodehaemonchus AT brunocenevivafornazari synergicinteractionofaromaticcompoundsanetholandcarvoneagainstnematodehaemonchus AT rafaelcanonencoaraujo synergicinteractionofaromaticcompoundsanetholandcarvoneagainstnematodehaemonchus AT helderlouvandini synergicinteractionofaromaticcompoundsanetholandcarvoneagainstnematodehaemonchus AT ricardolopesdiasdacosta synergicinteractionofaromaticcompoundsanetholandcarvoneagainstnematodehaemonchus AT maurosartoribueno synergicinteractionofaromaticcompoundsanetholandcarvoneagainstnematodehaemonchus AT ceciliajoseverissimo synergicinteractionofaromaticcompoundsanetholandcarvoneagainstnematodehaemonchus |