Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia
Cucurbits are important crops in the world. However, leaf curl disease constrains their production. Here, begomovirus diversity and pathogenicity associated with the disease in Malaysia were studied based on 49 begomovirus-detected out of 69 symptomatic plants from seven cucurbit crops in 15 locatio...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/11/2396 |
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| author | Yu-Jeng Chen Hsuan-Chun Lai Chung-Cheng Lin Zhuan Yi Neoh Wen-Shi Tsai |
| author_facet | Yu-Jeng Chen Hsuan-Chun Lai Chung-Cheng Lin Zhuan Yi Neoh Wen-Shi Tsai |
| author_sort | Yu-Jeng Chen |
| collection | DOAJ |
| description | Cucurbits are important crops in the world. However, leaf curl disease constrains their production. Here, begomovirus diversity and pathogenicity associated with the disease in Malaysia were studied based on 49 begomovirus-detected out of 69 symptomatic plants from seven cucurbit crops in 15 locations during 2016 and 2017. The presence of <i>Squash leaf curl China virus</i> (SLCCNV) and <i>Tomato leaf curl New Delhi virus</i> (ToLCNDV) were confirmed by virus detection by polymerase chain reaction, viral DNA sequence analysis and specific detection of the viral components. ToLCNDV Malaysian isolates were further distinguished into strains A, B, C and D. Virus co-infection was detected in bitter gourd, bottle gourd and squash. Among them, eight bitter gourd samples were detected without SLCCNV DNA-A. However, one bottle gourd and five squash samples were without ToLCNDV DNA-B. Pseudorecombination of ToLCNDV DNA-A and SLCCNV DNA-B was detected in two bitter gourd samples. The pathogenic viruses and pseudorecombinants were confirmed by agroinoculation. The viral DNA-B influencing on symptomology and host range was also confirmed. The results strengthen the epidemic of cucurbit-infecting begomovirus in Malaysia as well as Southeast Asia. Especially, the natural pseudorecombinant of begomovirus that extends host range and causes severe symptom implies a threat to crops. |
| first_indexed | 2024-03-10T05:07:55Z |
| format | Article |
| id | doaj.art-a418bbef67b74e79b09479a67dae184d |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T05:07:55Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-a418bbef67b74e79b09479a67dae184d2023-11-23T01:04:59ZengMDPI AGPlants2223-77472021-11-011011239610.3390/plants10112396Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in MalaysiaYu-Jeng Chen0Hsuan-Chun Lai1Chung-Cheng Lin2Zhuan Yi Neoh3Wen-Shi Tsai4Department of Plant Medicine, National Chiayi University, Chiayi City 600355, TaiwanDepartment of Plant Medicine, National Chiayi University, Chiayi City 600355, TaiwanClover Seed Company Ltd., Hong KongDepartment of Plant Medicine, National Chiayi University, Chiayi City 600355, TaiwanDepartment of Plant Medicine, National Chiayi University, Chiayi City 600355, TaiwanCucurbits are important crops in the world. However, leaf curl disease constrains their production. Here, begomovirus diversity and pathogenicity associated with the disease in Malaysia were studied based on 49 begomovirus-detected out of 69 symptomatic plants from seven cucurbit crops in 15 locations during 2016 and 2017. The presence of <i>Squash leaf curl China virus</i> (SLCCNV) and <i>Tomato leaf curl New Delhi virus</i> (ToLCNDV) were confirmed by virus detection by polymerase chain reaction, viral DNA sequence analysis and specific detection of the viral components. ToLCNDV Malaysian isolates were further distinguished into strains A, B, C and D. Virus co-infection was detected in bitter gourd, bottle gourd and squash. Among them, eight bitter gourd samples were detected without SLCCNV DNA-A. However, one bottle gourd and five squash samples were without ToLCNDV DNA-B. Pseudorecombination of ToLCNDV DNA-A and SLCCNV DNA-B was detected in two bitter gourd samples. The pathogenic viruses and pseudorecombinants were confirmed by agroinoculation. The viral DNA-B influencing on symptomology and host range was also confirmed. The results strengthen the epidemic of cucurbit-infecting begomovirus in Malaysia as well as Southeast Asia. Especially, the natural pseudorecombinant of begomovirus that extends host range and causes severe symptom implies a threat to crops.https://www.mdpi.com/2223-7747/10/11/2396<i>Squash leaf curl China virus</i><i>Tomato leaf curl New Delhi virus</i>agroinoculationpolymerase chain reactionhost range |
| spellingShingle | Yu-Jeng Chen Hsuan-Chun Lai Chung-Cheng Lin Zhuan Yi Neoh Wen-Shi Tsai Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia Plants <i>Squash leaf curl China virus</i> <i>Tomato leaf curl New Delhi virus</i> agroinoculation polymerase chain reaction host range |
| title | Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia |
| title_full | Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia |
| title_fullStr | Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia |
| title_full_unstemmed | Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia |
| title_short | Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia |
| title_sort | genetic diversity pathogenicity and pseudorecombination of cucurbit infecting begomoviruses in malaysia |
| topic | <i>Squash leaf curl China virus</i> <i>Tomato leaf curl New Delhi virus</i> agroinoculation polymerase chain reaction host range |
| url | https://www.mdpi.com/2223-7747/10/11/2396 |
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