Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy
Purpose: The aim of this research is to see how Melissa officinalis L. affects the death pathways of MCF-7 breast cancer cells in vitro. Materials and Methods: In order to determine the cytotoxic activity and IC50 value of the 70% methanolic extract prepared from the aerial parts of Melissa officina...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Cukurova University
2022-06-01
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| Series: | Cukurova Medical Journal |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/download/article-file/2322481 |
| _version_ | 1797919881123856384 |
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| author | Nazmiye Bitgen Arzu Yay Perihan Gürbüz Pınar Alişan Suna Gözde Özge Önder Münevver Baran |
| author_facet | Nazmiye Bitgen Arzu Yay Perihan Gürbüz Pınar Alişan Suna Gözde Özge Önder Münevver Baran |
| author_sort | Nazmiye Bitgen |
| collection | DOAJ |
| description | Purpose: The aim of this research is to see how Melissa officinalis L. affects the death pathways of MCF-7 breast cancer cells in vitro.
Materials and Methods: In order to determine the cytotoxic activity and IC50 value of the 70% methanolic extract prepared from the aerial parts of Melissa officinalis, on the MCF-7 breast cancer cell line; the extract was applied at different concentrations and times and MTT analysis was performed. Apoptotic effects of Melissa officinalis on breast cancer cells at the determined IC50 dose were revealed by TUNEL method, and autophagic effects were revealed using Beclin-1 and LC3 immunofluorescence staining method.
Results: When the effects of Melissa officinalis on MCF-7 breast cancer cells were evaluated, it was observed that the cell viability of the MCF-7 breast cancer cell line was decreased in a dose- and time-dependent manner. In addition, it was observed that it induced apoptosis and increased the immunoreactivity of autophagy markers Beclin-1 and LC3.
Conclusion: Findings from the study showed that Melissa officinalis has a cytotoxic effect on MCF-7 breast cancer cells through both apoptosis and autophagy cell death pathways. These data will lead to further in vitro studies necessary to elucidate the antitumor mechanism of action. |
| first_indexed | 2024-04-10T13:52:20Z |
| format | Article |
| id | doaj.art-a41af148f3cc44099700c4339d3889ba |
| institution | Directory Open Access Journal |
| issn | 2602-3040 |
| language | English |
| last_indexed | 2024-04-10T13:52:20Z |
| publishDate | 2022-06-01 |
| publisher | Cukurova University |
| record_format | Article |
| series | Cukurova Medical Journal |
| spelling | doaj.art-a41af148f3cc44099700c4339d3889ba2023-02-15T16:10:38ZengCukurova UniversityCukurova Medical Journal2602-30402022-06-0147276577510.17826/cumj.109093048Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagyNazmiye Bitgen0Arzu Yay1Perihan Gürbüz2Pınar Alişan Suna3Gözde Özge Önder4Münevver Baran5Erciyes ÜniversitesiERCIYES UNIVERSITY, SCHOOL OF MEDICINEERCIYES UNIVERSITY, FACULTY OF PHARMACYERCIYES UNIVERSITY, SCHOOL OF MEDICINEERCIYES UNIVERSITY, SCHOOL OF MEDICINEERCIYES UNIVERSITY, FACULTY OF PHARMACYPurpose: The aim of this research is to see how Melissa officinalis L. affects the death pathways of MCF-7 breast cancer cells in vitro. Materials and Methods: In order to determine the cytotoxic activity and IC50 value of the 70% methanolic extract prepared from the aerial parts of Melissa officinalis, on the MCF-7 breast cancer cell line; the extract was applied at different concentrations and times and MTT analysis was performed. Apoptotic effects of Melissa officinalis on breast cancer cells at the determined IC50 dose were revealed by TUNEL method, and autophagic effects were revealed using Beclin-1 and LC3 immunofluorescence staining method. Results: When the effects of Melissa officinalis on MCF-7 breast cancer cells were evaluated, it was observed that the cell viability of the MCF-7 breast cancer cell line was decreased in a dose- and time-dependent manner. In addition, it was observed that it induced apoptosis and increased the immunoreactivity of autophagy markers Beclin-1 and LC3. Conclusion: Findings from the study showed that Melissa officinalis has a cytotoxic effect on MCF-7 breast cancer cells through both apoptosis and autophagy cell death pathways. These data will lead to further in vitro studies necessary to elucidate the antitumor mechanism of action.https://dergipark.org.tr/tr/download/article-file/2322481mcf-7 cell linemelissa officinalis l.apoptosisautophagy.mcf-7 cell linemelissa officinalis l.apoptosisautophagy |
| spellingShingle | Nazmiye Bitgen Arzu Yay Perihan Gürbüz Pınar Alişan Suna Gözde Özge Önder Münevver Baran Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy Cukurova Medical Journal mcf-7 cell line melissa officinalis l. apoptosis autophagy. mcf-7 cell line melissa officinalis l. apoptosis autophagy |
| title | Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy |
| title_full | Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy |
| title_fullStr | Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy |
| title_full_unstemmed | Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy |
| title_short | Effect of Melissa officinalis L. on human breast cancer cell line via apoptosis and autophagy |
| title_sort | effect of melissa officinalis l on human breast cancer cell line via apoptosis and autophagy |
| topic | mcf-7 cell line melissa officinalis l. apoptosis autophagy. mcf-7 cell line melissa officinalis l. apoptosis autophagy |
| url | https://dergipark.org.tr/tr/download/article-file/2322481 |
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