In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions
Abstract Background Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a contagious infectious disease that affects domestic and wild ruminants causing chronic inflammation of the intestine. MAP has proven to be very resistant to both physical and chemical...
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| Format: | Article |
| Language: | English |
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BMC
2018-11-01
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| Series: | BMC Microbiology |
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| Online Access: | http://link.springer.com/article/10.1186/s12866-018-1313-6 |
| _version_ | 1818229202368856064 |
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| author | P. Steuer C. Avilez C. Tejeda N. Gonzalez A. Ramirez-Reveco F. Ulloa A. Mella I. R. Grant M. T. Collins M. Salgado |
| author_facet | P. Steuer C. Avilez C. Tejeda N. Gonzalez A. Ramirez-Reveco F. Ulloa A. Mella I. R. Grant M. T. Collins M. Salgado |
| author_sort | P. Steuer |
| collection | DOAJ |
| description | Abstract Background Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a contagious infectious disease that affects domestic and wild ruminants causing chronic inflammation of the intestine. MAP has proven to be very resistant to both physical and chemical processes, making it difficult to control this pathogen. Based on the recognized antimicrobial properties of copper, the objective of this study was to evaluate the effectiveness of copper ions to reduce MAP numbers and/or MAP viability in a fluid matrix. Besides, methicillin-resistant Staphylococcus aureus (MRSA), and Escherichia coli were used as controls of the effectiveness of copper ions. MAP-spiked PBS was subjected to copper ions treatment at 24 V for 5 min and the PBS suspensions were sampled before and after treatment. MAP viability and quantification were determined using three complementary techniques: a phage amplification assay, MGIT culture and qPCR. Results Moderate numbers (103 CFU ml−1) of the two control bacteria were completely eliminated by treatment with copper ions. For MAP, copper ions treatment reduced both the viability and numbers of this pathogen. Phage assay information quickly showed that copper ions (24 V for 5 min) resulted in a significant reduction in viable MAP. MGIT culture results over time showed statistically significant differences in time-to-detection (TTD) values between PRE and POST treatment. MAP genome equivalent estimates for PBS suspensions indicated that MAP numbers were lower in samples POST-treatment with copper ions than PRE-treatment. Conclusions The use of copper ions resulted in a significant reduction of MAP in a liquid matrix, although some MAP survival on some occasions was observed. |
| first_indexed | 2024-12-12T10:14:51Z |
| format | Article |
| id | doaj.art-a4229dc7e3b94198a560f41d07fc233a |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-12T10:14:51Z |
| publishDate | 2018-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-a4229dc7e3b94198a560f41d07fc233a2022-12-22T00:27:41ZengBMCBMC Microbiology1471-21802018-11-011811710.1186/s12866-018-1313-6In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ionsP. Steuer0C. Avilez1C. Tejeda2N. Gonzalez3A. Ramirez-Reveco4F. Ulloa5A. Mella6I. R. Grant7M. T. Collins8M. Salgado9Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de ChileInstituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de ChileInstituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de ChileInstituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de ChileInstituto de Ciencia Animal, Universidad Austral de ChileInstituto de Bioquímica y Microbiología, Universidad Austral de ChileInstituto de Bioquímica y Microbiología, Universidad Austral de ChileInstitute for Global Food Security, School of Biological Sciences, Queen’s University BelfastDepartment of Pathobiological Sciences, School of Veterinary Medicine, University of WisconsinInstituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de ChileAbstract Background Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a contagious infectious disease that affects domestic and wild ruminants causing chronic inflammation of the intestine. MAP has proven to be very resistant to both physical and chemical processes, making it difficult to control this pathogen. Based on the recognized antimicrobial properties of copper, the objective of this study was to evaluate the effectiveness of copper ions to reduce MAP numbers and/or MAP viability in a fluid matrix. Besides, methicillin-resistant Staphylococcus aureus (MRSA), and Escherichia coli were used as controls of the effectiveness of copper ions. MAP-spiked PBS was subjected to copper ions treatment at 24 V for 5 min and the PBS suspensions were sampled before and after treatment. MAP viability and quantification were determined using three complementary techniques: a phage amplification assay, MGIT culture and qPCR. Results Moderate numbers (103 CFU ml−1) of the two control bacteria were completely eliminated by treatment with copper ions. For MAP, copper ions treatment reduced both the viability and numbers of this pathogen. Phage assay information quickly showed that copper ions (24 V for 5 min) resulted in a significant reduction in viable MAP. MGIT culture results over time showed statistically significant differences in time-to-detection (TTD) values between PRE and POST treatment. MAP genome equivalent estimates for PBS suspensions indicated that MAP numbers were lower in samples POST-treatment with copper ions than PRE-treatment. Conclusions The use of copper ions resulted in a significant reduction of MAP in a liquid matrix, although some MAP survival on some occasions was observed.http://link.springer.com/article/10.1186/s12866-018-1313-6Mycobacterium avium subsp. paratuberculosisCopper ionsPhosphate buffered salineMGIT culturePhage amplification assay |
| spellingShingle | P. Steuer C. Avilez C. Tejeda N. Gonzalez A. Ramirez-Reveco F. Ulloa A. Mella I. R. Grant M. T. Collins M. Salgado In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions BMC Microbiology Mycobacterium avium subsp. paratuberculosis Copper ions Phosphate buffered saline MGIT culture Phage amplification assay |
| title | In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions |
| title_full | In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions |
| title_fullStr | In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions |
| title_full_unstemmed | In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions |
| title_short | In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions |
| title_sort | in vitro inactivation of mycobacterium avium subsp paratuberculosis map by use of copper ions |
| topic | Mycobacterium avium subsp. paratuberculosis Copper ions Phosphate buffered saline MGIT culture Phage amplification assay |
| url | http://link.springer.com/article/10.1186/s12866-018-1313-6 |
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