Long-term Maintenance of CD4 T Cell Memory Responses to Malaria Antigens in Malian Children Coinfected with Schistosoma haematobium
Polyparasitism is common in the developing world. We have previously demonstrated that schistosomiasis-positive (SP) Malian children, aged 4–8 years, are protected from malaria compared to matched schistosomiasis-negative (SN) children. The effect of concomitant schistosomiasis upon acquisition of T...
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Frontiers Media S.A.
2018-02-01
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| Series: | Frontiers in Immunology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fimmu.2017.01995/full |
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| author | Kirsten E. Lyke Kirsten E. Lyke Abdoulaye Dabo Charles Arama Issa Diarra Christopher V. Plowe Christopher V. Plowe Ogobara K. Doumbo Marcelo B. Sztein |
| author_facet | Kirsten E. Lyke Kirsten E. Lyke Abdoulaye Dabo Charles Arama Issa Diarra Christopher V. Plowe Christopher V. Plowe Ogobara K. Doumbo Marcelo B. Sztein |
| author_sort | Kirsten E. Lyke |
| collection | DOAJ |
| description | Polyparasitism is common in the developing world. We have previously demonstrated that schistosomiasis-positive (SP) Malian children, aged 4–8 years, are protected from malaria compared to matched schistosomiasis-negative (SN) children. The effect of concomitant schistosomiasis upon acquisition of T cell memory is unknown. We examined antigen-specific T cell frequencies in 48 Malian children aged 4–14 to a pool of malaria blood stage antigens, and a pool of schistosomal antigens, at a time point during a malaria episode and at a convalescent time point ~6 months later, following cessation of malaria transmission. CD4+ T cell-derived memory responses, defined as one or more significant cytokine (IFN-γ, TNF-α, IL-2, and/or IL-17A) responses, was measured to schistoma antigens in 18/23 SP children at one or both time points, compared to 4/23 SN children (P < 0.0001). At the time of malaria infection, 12/24 SN children and 15/23 SP children (P = 0.29) stimulated with malaria antigens demonstrated memory recall as defined by CD4-derived cytokine production. This compares to 7/23 SN children and 16/23 SP children (P = 0.009) at the convalescent timepoint. 46.2% of cytokine-producing CD4+ T cells expressed a single cytokine after stimulation with malaria antigen during the malaria episode. This fell to 40.9% at follow-up with a compensatory rise of multifunctional cytokine secretion over time, a phenomenon consistent with memory maturation. The majority (53.2–59.5%) of responses derived from CD45RA−CD62L− effector memory T cells with little variation in the phenotype depending upon the time point or the study cohort. We conclude that detectable T cell memory responses can be measured against both malaria and schistosoma antigens and that the presence of Schistosoma haematobium may be associated with long-term maintenance of T memory to malaria. |
| first_indexed | 2024-12-14T17:49:10Z |
| format | Article |
| id | doaj.art-a42457d4d5164e7aac555bae71c91f9b |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-14T17:49:10Z |
| publishDate | 2018-02-01 |
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| series | Frontiers in Immunology |
| spelling | doaj.art-a42457d4d5164e7aac555bae71c91f9b2022-12-21T22:52:41ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-02-01810.3389/fimmu.2017.01995323622Long-term Maintenance of CD4 T Cell Memory Responses to Malaria Antigens in Malian Children Coinfected with Schistosoma haematobiumKirsten E. Lyke0Kirsten E. Lyke1Abdoulaye Dabo2Charles Arama3Issa Diarra4Christopher V. Plowe5Christopher V. Plowe6Ogobara K. Doumbo7Marcelo B. Sztein8Center for Vaccine Development, Institute for Global Health, School of Medicine, University of Maryland, Baltimore, MD, United StatesDivision of Malaria Research, Institute for Global Health, School of Medicine, University of Maryland, Baltimore, MD, United StatesMalaria Research and Training Center, International Centers for Excellence in Research (NIH), University of Science Techniques and Technologies of Bamako, Bamako, MaliMalaria Research and Training Center, International Centers for Excellence in Research (NIH), University of Science Techniques and Technologies of Bamako, Bamako, MaliMalaria Research and Training Center, International Centers for Excellence in Research (NIH), University of Science Techniques and Technologies of Bamako, Bamako, MaliCenter for Vaccine Development, Institute for Global Health, School of Medicine, University of Maryland, Baltimore, MD, United StatesDivision of Malaria Research, Institute for Global Health, School of Medicine, University of Maryland, Baltimore, MD, United StatesMalaria Research and Training Center, International Centers for Excellence in Research (NIH), University of Science Techniques and Technologies of Bamako, Bamako, MaliCenter for Vaccine Development, Institute for Global Health, School of Medicine, University of Maryland, Baltimore, MD, United StatesPolyparasitism is common in the developing world. We have previously demonstrated that schistosomiasis-positive (SP) Malian children, aged 4–8 years, are protected from malaria compared to matched schistosomiasis-negative (SN) children. The effect of concomitant schistosomiasis upon acquisition of T cell memory is unknown. We examined antigen-specific T cell frequencies in 48 Malian children aged 4–14 to a pool of malaria blood stage antigens, and a pool of schistosomal antigens, at a time point during a malaria episode and at a convalescent time point ~6 months later, following cessation of malaria transmission. CD4+ T cell-derived memory responses, defined as one or more significant cytokine (IFN-γ, TNF-α, IL-2, and/or IL-17A) responses, was measured to schistoma antigens in 18/23 SP children at one or both time points, compared to 4/23 SN children (P < 0.0001). At the time of malaria infection, 12/24 SN children and 15/23 SP children (P = 0.29) stimulated with malaria antigens demonstrated memory recall as defined by CD4-derived cytokine production. This compares to 7/23 SN children and 16/23 SP children (P = 0.009) at the convalescent timepoint. 46.2% of cytokine-producing CD4+ T cells expressed a single cytokine after stimulation with malaria antigen during the malaria episode. This fell to 40.9% at follow-up with a compensatory rise of multifunctional cytokine secretion over time, a phenomenon consistent with memory maturation. The majority (53.2–59.5%) of responses derived from CD45RA−CD62L− effector memory T cells with little variation in the phenotype depending upon the time point or the study cohort. We conclude that detectable T cell memory responses can be measured against both malaria and schistosoma antigens and that the presence of Schistosoma haematobium may be associated with long-term maintenance of T memory to malaria.http://journal.frontiersin.org/article/10.3389/fimmu.2017.01995/fullmalariaschistosomiasiscoinfectionmultifunctional T cellsT cell memorymemory |
| spellingShingle | Kirsten E. Lyke Kirsten E. Lyke Abdoulaye Dabo Charles Arama Issa Diarra Christopher V. Plowe Christopher V. Plowe Ogobara K. Doumbo Marcelo B. Sztein Long-term Maintenance of CD4 T Cell Memory Responses to Malaria Antigens in Malian Children Coinfected with Schistosoma haematobium Frontiers in Immunology malaria schistosomiasis coinfection multifunctional T cells T cell memory memory |
| title | Long-term Maintenance of CD4 T Cell Memory Responses to Malaria Antigens in Malian Children Coinfected with Schistosoma haematobium |
| title_full | Long-term Maintenance of CD4 T Cell Memory Responses to Malaria Antigens in Malian Children Coinfected with Schistosoma haematobium |
| title_fullStr | Long-term Maintenance of CD4 T Cell Memory Responses to Malaria Antigens in Malian Children Coinfected with Schistosoma haematobium |
| title_full_unstemmed | Long-term Maintenance of CD4 T Cell Memory Responses to Malaria Antigens in Malian Children Coinfected with Schistosoma haematobium |
| title_short | Long-term Maintenance of CD4 T Cell Memory Responses to Malaria Antigens in Malian Children Coinfected with Schistosoma haematobium |
| title_sort | long term maintenance of cd4 t cell memory responses to malaria antigens in malian children coinfected with schistosoma haematobium |
| topic | malaria schistosomiasis coinfection multifunctional T cells T cell memory memory |
| url | http://journal.frontiersin.org/article/10.3389/fimmu.2017.01995/full |
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