Activating and repressing gene expression between chromosomes during stochastic fate specification
Summary: DNA elements act across long genomic distances to regulate gene expression. During transvection in Drosophila, DNA elements on one allele of a gene act between chromosomes to regulate expression of the other allele. Little is known about the biological roles and developmental regulation of...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124722018095 |
| _version_ | 1797961135634251776 |
|---|---|
| author | Elizabeth A. Urban Chaim Chernoff Kayla Viets Layng Jeong Han Caitlin Anderson Daniel Konzman Robert J. Johnston, Jr. |
| author_facet | Elizabeth A. Urban Chaim Chernoff Kayla Viets Layng Jeong Han Caitlin Anderson Daniel Konzman Robert J. Johnston, Jr. |
| author_sort | Elizabeth A. Urban |
| collection | DOAJ |
| description | Summary: DNA elements act across long genomic distances to regulate gene expression. During transvection in Drosophila, DNA elements on one allele of a gene act between chromosomes to regulate expression of the other allele. Little is known about the biological roles and developmental regulation of transvection. Here, we study the stochastic expression of spineless (ss) in photoreceptors in the fly eye to understand transvection. We determine a biological role for transvection in regulating expression of naturally occurring ss alleles. We identify DNA elements required for activating and repressing transvection. Different enhancers participate in transvection at different times during development to promote gene expression and specify cell fates. Bringing a silencer element on a heterologous chromosome into proximity with the ss locus “reconstitutes” the gene, leading to repression. Our studies show that transvection regulates gene expression via distinct DNA elements at specific timepoints in development, with implications for genome organization and architecture. |
| first_indexed | 2024-04-11T00:54:40Z |
| format | Article |
| id | doaj.art-a424a18fcf5242ddbec0cabed6a82fd3 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-11T00:54:40Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-a424a18fcf5242ddbec0cabed6a82fd32023-01-05T06:47:00ZengElsevierCell Reports2211-12472023-01-01421111910Activating and repressing gene expression between chromosomes during stochastic fate specificationElizabeth A. Urban0Chaim Chernoff1Kayla Viets Layng2Jeong Han3Caitlin Anderson4Daniel Konzman5Robert J. Johnston, Jr.6Department of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USADepartment of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USADepartment of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USADepartment of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USADepartment of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USADepartment of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USADepartment of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USA; Corresponding authorSummary: DNA elements act across long genomic distances to regulate gene expression. During transvection in Drosophila, DNA elements on one allele of a gene act between chromosomes to regulate expression of the other allele. Little is known about the biological roles and developmental regulation of transvection. Here, we study the stochastic expression of spineless (ss) in photoreceptors in the fly eye to understand transvection. We determine a biological role for transvection in regulating expression of naturally occurring ss alleles. We identify DNA elements required for activating and repressing transvection. Different enhancers participate in transvection at different times during development to promote gene expression and specify cell fates. Bringing a silencer element on a heterologous chromosome into proximity with the ss locus “reconstitutes” the gene, leading to repression. Our studies show that transvection regulates gene expression via distinct DNA elements at specific timepoints in development, with implications for genome organization and architecture.http://www.sciencedirect.com/science/article/pii/S2211124722018095CP: Molecular biology |
| spellingShingle | Elizabeth A. Urban Chaim Chernoff Kayla Viets Layng Jeong Han Caitlin Anderson Daniel Konzman Robert J. Johnston, Jr. Activating and repressing gene expression between chromosomes during stochastic fate specification Cell Reports CP: Molecular biology |
| title | Activating and repressing gene expression between chromosomes during stochastic fate specification |
| title_full | Activating and repressing gene expression between chromosomes during stochastic fate specification |
| title_fullStr | Activating and repressing gene expression between chromosomes during stochastic fate specification |
| title_full_unstemmed | Activating and repressing gene expression between chromosomes during stochastic fate specification |
| title_short | Activating and repressing gene expression between chromosomes during stochastic fate specification |
| title_sort | activating and repressing gene expression between chromosomes during stochastic fate specification |
| topic | CP: Molecular biology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124722018095 |
| work_keys_str_mv | AT elizabethaurban activatingandrepressinggeneexpressionbetweenchromosomesduringstochasticfatespecification AT chaimchernoff activatingandrepressinggeneexpressionbetweenchromosomesduringstochasticfatespecification AT kaylavietslayng activatingandrepressinggeneexpressionbetweenchromosomesduringstochasticfatespecification AT jeonghan activatingandrepressinggeneexpressionbetweenchromosomesduringstochasticfatespecification AT caitlinanderson activatingandrepressinggeneexpressionbetweenchromosomesduringstochasticfatespecification AT danielkonzman activatingandrepressinggeneexpressionbetweenchromosomesduringstochasticfatespecification AT robertjjohnstonjr activatingandrepressinggeneexpressionbetweenchromosomesduringstochasticfatespecification |