A novel mouse model of Campylobacter jejuni enteropathy and diarrhea.
Campylobacter infections are among the leading bacterial causes of diarrhea and of 'environmental enteropathy' (EE) and growth failure worldwide. However, the lack of an inexpensive small animal model of enteric disease with Campylobacter has been a major limitation for understanding its p...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-03-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1007083&type=printable |
| _version_ | 1826579506253529088 |
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| author | Natasa Giallourou Gregory L Medlock David T Bolick Pedro Hqs Medeiros Solanka E Ledwaba Glynis L Kolling Kenneth Tung Patricia Guerry Jonathan R Swann Richard L Guerrant |
| author_facet | Natasa Giallourou Gregory L Medlock David T Bolick Pedro Hqs Medeiros Solanka E Ledwaba Glynis L Kolling Kenneth Tung Patricia Guerry Jonathan R Swann Richard L Guerrant |
| author_sort | Natasa Giallourou |
| collection | DOAJ |
| description | Campylobacter infections are among the leading bacterial causes of diarrhea and of 'environmental enteropathy' (EE) and growth failure worldwide. However, the lack of an inexpensive small animal model of enteric disease with Campylobacter has been a major limitation for understanding its pathogenesis, interventions or vaccine development. We describe a robust standard mouse model that can exhibit reproducible bloody diarrhea or growth failure, depending on the zinc or protein deficient diet and on antibiotic alteration of normal microbiota prior to infection. Zinc deficiency and the use of antibiotics create a niche for Campylobacter infection to establish by narrowing the metabolic flexibility of these mice for pathogen clearance and by promoting intestinal and systemic inflammation. Several biomarkers and intestinal pathology in this model also mimic those seen in human disease. This model provides a novel tool to test specific hypotheses regarding disease pathogenesis as well as vaccine development that is currently in progress. |
| first_indexed | 2024-12-23T02:13:31Z |
| format | Article |
| id | doaj.art-a428cde70ff34eba80fd09b32b983ceb |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2025-03-14T14:19:21Z |
| publishDate | 2018-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-a428cde70ff34eba80fd09b32b983ceb2025-02-27T05:31:54ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742018-03-01143e100708310.1371/journal.ppat.1007083A novel mouse model of Campylobacter jejuni enteropathy and diarrhea.Natasa GiallourouGregory L MedlockDavid T BolickPedro Hqs MedeirosSolanka E LedwabaGlynis L KollingKenneth TungPatricia GuerryJonathan R SwannRichard L GuerrantCampylobacter infections are among the leading bacterial causes of diarrhea and of 'environmental enteropathy' (EE) and growth failure worldwide. However, the lack of an inexpensive small animal model of enteric disease with Campylobacter has been a major limitation for understanding its pathogenesis, interventions or vaccine development. We describe a robust standard mouse model that can exhibit reproducible bloody diarrhea or growth failure, depending on the zinc or protein deficient diet and on antibiotic alteration of normal microbiota prior to infection. Zinc deficiency and the use of antibiotics create a niche for Campylobacter infection to establish by narrowing the metabolic flexibility of these mice for pathogen clearance and by promoting intestinal and systemic inflammation. Several biomarkers and intestinal pathology in this model also mimic those seen in human disease. This model provides a novel tool to test specific hypotheses regarding disease pathogenesis as well as vaccine development that is currently in progress.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1007083&type=printable |
| spellingShingle | Natasa Giallourou Gregory L Medlock David T Bolick Pedro Hqs Medeiros Solanka E Ledwaba Glynis L Kolling Kenneth Tung Patricia Guerry Jonathan R Swann Richard L Guerrant A novel mouse model of Campylobacter jejuni enteropathy and diarrhea. PLoS Pathogens |
| title | A novel mouse model of Campylobacter jejuni enteropathy and diarrhea. |
| title_full | A novel mouse model of Campylobacter jejuni enteropathy and diarrhea. |
| title_fullStr | A novel mouse model of Campylobacter jejuni enteropathy and diarrhea. |
| title_full_unstemmed | A novel mouse model of Campylobacter jejuni enteropathy and diarrhea. |
| title_short | A novel mouse model of Campylobacter jejuni enteropathy and diarrhea. |
| title_sort | novel mouse model of campylobacter jejuni enteropathy and diarrhea |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1007083&type=printable |
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