Alginate Core–Shell Capsules for 3D Cultivation of Adipose-Derived Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) are primary candidates in tissue engineering and stem cell therapies due to their intriguing regenerative and immunomodulatory potential. Their ability to self-assemble into three-dimensional (3D) aggregates further improves some of their therapeutic properties, e.g., d...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/9/2/66 |
| _version_ | 1797482681754189824 |
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| author | Sabrina Nebel Manuel Lux Sonja Kuth Faina Bider Wolf Dietrich Dominik Egger Aldo R. Boccaccini Cornelia Kasper |
| author_facet | Sabrina Nebel Manuel Lux Sonja Kuth Faina Bider Wolf Dietrich Dominik Egger Aldo R. Boccaccini Cornelia Kasper |
| author_sort | Sabrina Nebel |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) are primary candidates in tissue engineering and stem cell therapies due to their intriguing regenerative and immunomodulatory potential. Their ability to self-assemble into three-dimensional (3D) aggregates further improves some of their therapeutic properties, e.g., differentiation potential, secretion of cytokines, and homing capacity after administration. However, high hydrodynamic shear forces and the resulting mechanical stresses within commercially available dynamic cultivation systems can decrease their regenerative properties. Cells embedded within a polymer matrix, however, lack cell-to-cell interactions found in their physiological environment. Here, we present a “semi scaffold-free” approach to protect the cells from high shear forces by a physical barrier, but still allow formation of a 3D structure with in vivo-like cell-to-cell contacts. We highlight a relatively simple method to create core–shell capsules by inverse gelation. The capsules consist of an outer barrier made from sodium alginate, which allows for nutrient and waste diffusion and an inner compartment for direct cell-cell interactions. Next to capsule characterization, a harvesting procedure was established and viability and proliferation of human adipose-derived MSCs were investigated. In the future, this encapsulation and cultivation technique might be used for MSC-expansion in scalable dynamic bioreactor systems, facilitating downstream procedures, such as cell harvest and differentiation into mature tissue grafts. |
| first_indexed | 2024-03-09T22:36:00Z |
| format | Article |
| id | doaj.art-a42900fa66ef4dbeb9a3c222f8561d5c |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T22:36:00Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-a42900fa66ef4dbeb9a3c222f8561d5c2023-11-23T18:48:34ZengMDPI AGBioengineering2306-53542022-02-01926610.3390/bioengineering9020066Alginate Core–Shell Capsules for 3D Cultivation of Adipose-Derived Mesenchymal Stem CellsSabrina Nebel0Manuel Lux1Sonja Kuth2Faina Bider3Wolf Dietrich4Dominik Egger5Aldo R. Boccaccini6Cornelia Kasper7Institute of Cell and Tissue Culture Technologies, Department of Biotechnology, University of Natural Resources and Life Sciences BOKU Vienna, 1190 Vienna, AustriaInstitute of Cell and Tissue Culture Technologies, Department of Biotechnology, University of Natural Resources and Life Sciences BOKU Vienna, 1190 Vienna, AustriaInstitute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, GermanyInstitute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, GermanyDepartment of Gynecology and Obstetrics, Karl Landsteiner University of Health Sciences, Alter Ziegelweg 10, 3430 Tulln, AustriaInstitute of Cell and Tissue Culture Technologies, Department of Biotechnology, University of Natural Resources and Life Sciences BOKU Vienna, 1190 Vienna, AustriaInstitute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, GermanyInstitute of Cell and Tissue Culture Technologies, Department of Biotechnology, University of Natural Resources and Life Sciences BOKU Vienna, 1190 Vienna, AustriaMesenchymal stem cells (MSCs) are primary candidates in tissue engineering and stem cell therapies due to their intriguing regenerative and immunomodulatory potential. Their ability to self-assemble into three-dimensional (3D) aggregates further improves some of their therapeutic properties, e.g., differentiation potential, secretion of cytokines, and homing capacity after administration. However, high hydrodynamic shear forces and the resulting mechanical stresses within commercially available dynamic cultivation systems can decrease their regenerative properties. Cells embedded within a polymer matrix, however, lack cell-to-cell interactions found in their physiological environment. Here, we present a “semi scaffold-free” approach to protect the cells from high shear forces by a physical barrier, but still allow formation of a 3D structure with in vivo-like cell-to-cell contacts. We highlight a relatively simple method to create core–shell capsules by inverse gelation. The capsules consist of an outer barrier made from sodium alginate, which allows for nutrient and waste diffusion and an inner compartment for direct cell-cell interactions. Next to capsule characterization, a harvesting procedure was established and viability and proliferation of human adipose-derived MSCs were investigated. In the future, this encapsulation and cultivation technique might be used for MSC-expansion in scalable dynamic bioreactor systems, facilitating downstream procedures, such as cell harvest and differentiation into mature tissue grafts.https://www.mdpi.com/2306-5354/9/2/66mesenchymal stem cellscore–shell capsule3D cell cultivation technologiescell expansionalginate |
| spellingShingle | Sabrina Nebel Manuel Lux Sonja Kuth Faina Bider Wolf Dietrich Dominik Egger Aldo R. Boccaccini Cornelia Kasper Alginate Core–Shell Capsules for 3D Cultivation of Adipose-Derived Mesenchymal Stem Cells Bioengineering mesenchymal stem cells core–shell capsule 3D cell cultivation technologies cell expansion alginate |
| title | Alginate Core–Shell Capsules for 3D Cultivation of Adipose-Derived Mesenchymal Stem Cells |
| title_full | Alginate Core–Shell Capsules for 3D Cultivation of Adipose-Derived Mesenchymal Stem Cells |
| title_fullStr | Alginate Core–Shell Capsules for 3D Cultivation of Adipose-Derived Mesenchymal Stem Cells |
| title_full_unstemmed | Alginate Core–Shell Capsules for 3D Cultivation of Adipose-Derived Mesenchymal Stem Cells |
| title_short | Alginate Core–Shell Capsules for 3D Cultivation of Adipose-Derived Mesenchymal Stem Cells |
| title_sort | alginate core shell capsules for 3d cultivation of adipose derived mesenchymal stem cells |
| topic | mesenchymal stem cells core–shell capsule 3D cell cultivation technologies cell expansion alginate |
| url | https://www.mdpi.com/2306-5354/9/2/66 |
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