Incorporation of <i>Limosilactobacillus fermentum</i> UCO-979C with Anti-<i>Helicobacter pylori</i> and Immunomodulatory Activities in Various Ice Cream Bases
<i>Limosilactobacillus fermentum</i> UCO-979C is a probiotic strain possessing anti-<i>Helicobacter pylori</i> and immunomodulatory activity. The aim of this work was to examine if this strain maintains its probiotic properties and its viability when added to dairy-based ice...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/3/333 |
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| author | Cristina Paucar-Carrión Marcela Espinoza-Monje Cristian Gutiérrez-Zamorano Kimberly Sánchez-Alonzo Romina I. Carvajal Cristian Rogel-Castillo Katia Sáez-Carrillo Apolinaria García-Cancino |
| author_facet | Cristina Paucar-Carrión Marcela Espinoza-Monje Cristian Gutiérrez-Zamorano Kimberly Sánchez-Alonzo Romina I. Carvajal Cristian Rogel-Castillo Katia Sáez-Carrillo Apolinaria García-Cancino |
| author_sort | Cristina Paucar-Carrión |
| collection | DOAJ |
| description | <i>Limosilactobacillus fermentum</i> UCO-979C is a probiotic strain possessing anti-<i>Helicobacter pylori</i> and immunomodulatory activity. The aim of this work was to examine if this strain maintains its probiotic properties and its viability when added to dairy-based ice creams (cookies and cream, Greek yogurt, and chocolate with brownie) or to fruit-based ice creams (pineapple and raspberry) stored at −18 °C for 90 days. The probiotic anti-<i>H. pylori</i> activity using the well diffusion test, its immunomodulatory activity was measured using transforming growth factor beta 1 (TGF-β<sub>1</sub>) cytokine production by human gastric adenocarcinoma (AGS) cells, and its viability was measured using the microdrop technique. Assays were performed in triplicate. The <i>L. fermentum</i> UCO-979C strain maintained strong anti-<i>H. pylori</i> activity in dairy-based ice creams and mild activity in fruit-based ice cream. The production of pro-inflammatory cytokine TGF-β<sub>1</sub> on AGS cells was higher in the probiotic recovered from Greek yogurt ice cream, maintaining a viability exceeding 10<sup>7</sup> colony-forming units/mL. The addition of the probiotic to ice creams did not significantly influence the physicochemical properties of the product. These data show the great potential of the <i>L. fermentum</i> UCO-979C strain in producing probiotic dairy-based and fruit-based ice creams. |
| first_indexed | 2024-03-09T23:53:46Z |
| format | Article |
| id | doaj.art-a42dbd7e482c4a99bcda0ccbf1045059 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T23:53:46Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-a42dbd7e482c4a99bcda0ccbf10450592023-11-23T16:29:10ZengMDPI AGFoods2304-81582022-01-0111333310.3390/foods11030333Incorporation of <i>Limosilactobacillus fermentum</i> UCO-979C with Anti-<i>Helicobacter pylori</i> and Immunomodulatory Activities in Various Ice Cream BasesCristina Paucar-Carrión0Marcela Espinoza-Monje1Cristian Gutiérrez-Zamorano2Kimberly Sánchez-Alonzo3Romina I. Carvajal4Cristian Rogel-Castillo5Katia Sáez-Carrillo6Apolinaria García-Cancino7Laboratory of Bacterial Pathogenicity, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción 4070386, ChileLaboratory of Bacterial Pathogenicity, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción 4070386, ChileLaboratory of Bacterial Pathogenicity, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción 4070386, ChileLaboratory of Bacterial Pathogenicity, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción 4070386, ChileLaboratory of Bacterial Pathogenicity, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción 4070386, ChileDepartment of Food Science and Technology, Faculty of Pharmacy, Universidad de Concepción, Concepción 4070386, ChileDepartment of Statistics, Faculty of Physical and Mathematical Sciences, Universidad de Concepción, Concepción 4070386, ChileLaboratory of Bacterial Pathogenicity, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción 4070386, Chile<i>Limosilactobacillus fermentum</i> UCO-979C is a probiotic strain possessing anti-<i>Helicobacter pylori</i> and immunomodulatory activity. The aim of this work was to examine if this strain maintains its probiotic properties and its viability when added to dairy-based ice creams (cookies and cream, Greek yogurt, and chocolate with brownie) or to fruit-based ice creams (pineapple and raspberry) stored at −18 °C for 90 days. The probiotic anti-<i>H. pylori</i> activity using the well diffusion test, its immunomodulatory activity was measured using transforming growth factor beta 1 (TGF-β<sub>1</sub>) cytokine production by human gastric adenocarcinoma (AGS) cells, and its viability was measured using the microdrop technique. Assays were performed in triplicate. The <i>L. fermentum</i> UCO-979C strain maintained strong anti-<i>H. pylori</i> activity in dairy-based ice creams and mild activity in fruit-based ice cream. The production of pro-inflammatory cytokine TGF-β<sub>1</sub> on AGS cells was higher in the probiotic recovered from Greek yogurt ice cream, maintaining a viability exceeding 10<sup>7</sup> colony-forming units/mL. The addition of the probiotic to ice creams did not significantly influence the physicochemical properties of the product. These data show the great potential of the <i>L. fermentum</i> UCO-979C strain in producing probiotic dairy-based and fruit-based ice creams.https://www.mdpi.com/2304-8158/11/3/333probiotics<i>Limosilactobacillus fermentum</i> UCO-979C<i>Helicobacter pylori</i>anti-<i>H. pylori</i> activityice creamdairy food |
| spellingShingle | Cristina Paucar-Carrión Marcela Espinoza-Monje Cristian Gutiérrez-Zamorano Kimberly Sánchez-Alonzo Romina I. Carvajal Cristian Rogel-Castillo Katia Sáez-Carrillo Apolinaria García-Cancino Incorporation of <i>Limosilactobacillus fermentum</i> UCO-979C with Anti-<i>Helicobacter pylori</i> and Immunomodulatory Activities in Various Ice Cream Bases Foods probiotics <i>Limosilactobacillus fermentum</i> UCO-979C <i>Helicobacter pylori</i> anti-<i>H. pylori</i> activity ice cream dairy food |
| title | Incorporation of <i>Limosilactobacillus fermentum</i> UCO-979C with Anti-<i>Helicobacter pylori</i> and Immunomodulatory Activities in Various Ice Cream Bases |
| title_full | Incorporation of <i>Limosilactobacillus fermentum</i> UCO-979C with Anti-<i>Helicobacter pylori</i> and Immunomodulatory Activities in Various Ice Cream Bases |
| title_fullStr | Incorporation of <i>Limosilactobacillus fermentum</i> UCO-979C with Anti-<i>Helicobacter pylori</i> and Immunomodulatory Activities in Various Ice Cream Bases |
| title_full_unstemmed | Incorporation of <i>Limosilactobacillus fermentum</i> UCO-979C with Anti-<i>Helicobacter pylori</i> and Immunomodulatory Activities in Various Ice Cream Bases |
| title_short | Incorporation of <i>Limosilactobacillus fermentum</i> UCO-979C with Anti-<i>Helicobacter pylori</i> and Immunomodulatory Activities in Various Ice Cream Bases |
| title_sort | incorporation of i limosilactobacillus fermentum i uco 979c with anti i helicobacter pylori i and immunomodulatory activities in various ice cream bases |
| topic | probiotics <i>Limosilactobacillus fermentum</i> UCO-979C <i>Helicobacter pylori</i> anti-<i>H. pylori</i> activity ice cream dairy food |
| url | https://www.mdpi.com/2304-8158/11/3/333 |
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