The Rare Actinobacterium <i>Crossiella</i> sp. Is a Potential Source of New Bioactive Compounds with Activity against Bacteria and Fungi
Antimicrobial resistance has become a global problem in recent decades. A gradual reduction in drug discoveries has led to the current antimicrobial resistance crisis. Caves and other subsurface environments are underexplored thus far, and they represent indispensable ecological niches that could of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/8/1575 |
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| author | Jose Luis Gonzalez-Pimentel Irene Dominguez-Moñino Valme Jurado Leonila Laiz Ana Teresa Caldeira Cesareo Saiz-Jimenez |
| author_facet | Jose Luis Gonzalez-Pimentel Irene Dominguez-Moñino Valme Jurado Leonila Laiz Ana Teresa Caldeira Cesareo Saiz-Jimenez |
| author_sort | Jose Luis Gonzalez-Pimentel |
| collection | DOAJ |
| description | Antimicrobial resistance has become a global problem in recent decades. A gradual reduction in drug discoveries has led to the current antimicrobial resistance crisis. Caves and other subsurface environments are underexplored thus far, and they represent indispensable ecological niches that could offer new molecules of interest to medicine and biotechnology. We explored Spanish show caves to test the bioactivity of the bacteria dwelling in the walls and ceilings, as well as airborne bacteria. We reported the isolation of two strains of the genus <i>Crossiella</i>, likely representing a new species, isolated from Altamira Cave, Spain. In vitro and in silico analyses showed the inhibition of pathogenic Gram-positive and Gram-negative bacteria, and fungi, as well as the taxonomical distance of both strains from their closest relative, <i>Crossiella cryophila</i>. The presence of an exclusive combination of gene clusters involved in the synthesis of lanthipeptides, lasso peptides, nonribosomal peptides and polyketides indicates that species of this genus could represent a source of new compounds. Overall, there is promising evidence for antimicrobial discovery in subterranean environments, which increases the possibility of identifying new bioactive molecules. |
| first_indexed | 2024-03-09T04:03:50Z |
| format | Article |
| id | doaj.art-a42e3d3aa97b4bf9997d913baa380359 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T04:03:50Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a42e3d3aa97b4bf9997d913baa3803592023-12-03T14:09:18ZengMDPI AGMicroorganisms2076-26072022-08-01108157510.3390/microorganisms10081575The Rare Actinobacterium <i>Crossiella</i> sp. Is a Potential Source of New Bioactive Compounds with Activity against Bacteria and FungiJose Luis Gonzalez-Pimentel0Irene Dominguez-Moñino1Valme Jurado2Leonila Laiz3Ana Teresa Caldeira4Cesareo Saiz-Jimenez5Laboratorio Hercules, Universidade de Evora, 7004-516 Evora, PortugalInstituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, 41012 Sevilla, SpainInstituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, 41012 Sevilla, SpainInstituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, 41012 Sevilla, SpainLaboratorio Hercules, Universidade de Evora, 7004-516 Evora, PortugalInstituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, 41012 Sevilla, SpainAntimicrobial resistance has become a global problem in recent decades. A gradual reduction in drug discoveries has led to the current antimicrobial resistance crisis. Caves and other subsurface environments are underexplored thus far, and they represent indispensable ecological niches that could offer new molecules of interest to medicine and biotechnology. We explored Spanish show caves to test the bioactivity of the bacteria dwelling in the walls and ceilings, as well as airborne bacteria. We reported the isolation of two strains of the genus <i>Crossiella</i>, likely representing a new species, isolated from Altamira Cave, Spain. In vitro and in silico analyses showed the inhibition of pathogenic Gram-positive and Gram-negative bacteria, and fungi, as well as the taxonomical distance of both strains from their closest relative, <i>Crossiella cryophila</i>. The presence of an exclusive combination of gene clusters involved in the synthesis of lanthipeptides, lasso peptides, nonribosomal peptides and polyketides indicates that species of this genus could represent a source of new compounds. Overall, there is promising evidence for antimicrobial discovery in subterranean environments, which increases the possibility of identifying new bioactive molecules.https://www.mdpi.com/2076-2607/10/8/1575Altamira Cavebioactive compounds<i>Crossiella</i>predicted gene clustersSpanish show caves |
| spellingShingle | Jose Luis Gonzalez-Pimentel Irene Dominguez-Moñino Valme Jurado Leonila Laiz Ana Teresa Caldeira Cesareo Saiz-Jimenez The Rare Actinobacterium <i>Crossiella</i> sp. Is a Potential Source of New Bioactive Compounds with Activity against Bacteria and Fungi Microorganisms Altamira Cave bioactive compounds <i>Crossiella</i> predicted gene clusters Spanish show caves |
| title | The Rare Actinobacterium <i>Crossiella</i> sp. Is a Potential Source of New Bioactive Compounds with Activity against Bacteria and Fungi |
| title_full | The Rare Actinobacterium <i>Crossiella</i> sp. Is a Potential Source of New Bioactive Compounds with Activity against Bacteria and Fungi |
| title_fullStr | The Rare Actinobacterium <i>Crossiella</i> sp. Is a Potential Source of New Bioactive Compounds with Activity against Bacteria and Fungi |
| title_full_unstemmed | The Rare Actinobacterium <i>Crossiella</i> sp. Is a Potential Source of New Bioactive Compounds with Activity against Bacteria and Fungi |
| title_short | The Rare Actinobacterium <i>Crossiella</i> sp. Is a Potential Source of New Bioactive Compounds with Activity against Bacteria and Fungi |
| title_sort | rare actinobacterium i crossiella i sp is a potential source of new bioactive compounds with activity against bacteria and fungi |
| topic | Altamira Cave bioactive compounds <i>Crossiella</i> predicted gene clusters Spanish show caves |
| url | https://www.mdpi.com/2076-2607/10/8/1575 |
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