Fine-scale geographic difference of the endangered Big-headed Turtle (Platysternon megacephalum) fecal microbiota, and comparison with the syntopic Beale’s Eyed Turtle (Sacalia bealei)
Abstract Background Studies have elucidated the importance of gut microbiota for an organism, but we are still learning about the important influencing factors. Several factors have been identified in helping shape the microbiome of a host, and in this study we focus on two factors—geography and hos...
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-024-03227-2 |
| _version_ | 1827328389943394304 |
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| author | Jonathan J. Fong Yik-Hei Sung Li Ding |
| author_facet | Jonathan J. Fong Yik-Hei Sung Li Ding |
| author_sort | Jonathan J. Fong |
| collection | DOAJ |
| description | Abstract Background Studies have elucidated the importance of gut microbiota for an organism, but we are still learning about the important influencing factors. Several factors have been identified in helping shape the microbiome of a host, and in this study we focus on two factors—geography and host. We characterize the fecal microbiota of the Big-headed Turtle (Platysternon megacephalum) and compare across a relatively fine geographic scale (three populations within an 8-km radius) and between two syntopic hosts (P. megacephalum and Sacalia bealei). Both species are endangered, which limits the number of samples we include in the study. Despite this limitation, these data serve as baseline data for healthy, wild fecal microbiotas of two endangered turtle species to aid in conservation management. Results For geography, the beta diversity of fecal microbiota differed between the most distant sites. The genus Citrobacter significantly differs between sites, which may indicate a difference in food availability, environmental microbiota, or both. Also, we identify the common core microbiome for Platysternon across Hong Kong as the shared taxa across the three sites. Additionally, beta diversity differs between host species. Since the two species are from the same site and encounter the same environmental microbiota, we infer that there is a host effect on the fecal microbiota, such as diet or the recruitment of host-adapted bacteria. Lastly, functional analyses found metabolism pathways (KEGG level 1) to be the most common, and pathways (KEGG level 3) to be statistically significant between sites, but statistically indistinguishable between species at the same site. Conclusions We find that fecal microbiota can significantly differ at a fine geographic scale and between syntopic hosts. Also, the function of fecal microbiota seems to be strongly affected by geographic site, rather than species. This study characterizes the identity and function of the fecal microbiota of two endangered turtle species, from what is likely their last remaining wild populations. These data of healthy, wild fecal microbiota will serve as a baseline for comparison and contribute to the conservation of these two endangered species. |
| first_indexed | 2024-03-07T15:17:15Z |
| format | Article |
| id | doaj.art-a42f1ba8ae9b436cbf6e69c3812ce22a |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-07T15:17:15Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-a42f1ba8ae9b436cbf6e69c3812ce22a2024-03-05T17:51:33ZengBMCBMC Microbiology1471-21802024-02-0124111210.1186/s12866-024-03227-2Fine-scale geographic difference of the endangered Big-headed Turtle (Platysternon megacephalum) fecal microbiota, and comparison with the syntopic Beale’s Eyed Turtle (Sacalia bealei)Jonathan J. Fong0Yik-Hei Sung1Li Ding2Science Unit, Lingnan UniversityScience Unit, Lingnan UniversityMinistry of Education Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal UniversityAbstract Background Studies have elucidated the importance of gut microbiota for an organism, but we are still learning about the important influencing factors. Several factors have been identified in helping shape the microbiome of a host, and in this study we focus on two factors—geography and host. We characterize the fecal microbiota of the Big-headed Turtle (Platysternon megacephalum) and compare across a relatively fine geographic scale (three populations within an 8-km radius) and between two syntopic hosts (P. megacephalum and Sacalia bealei). Both species are endangered, which limits the number of samples we include in the study. Despite this limitation, these data serve as baseline data for healthy, wild fecal microbiotas of two endangered turtle species to aid in conservation management. Results For geography, the beta diversity of fecal microbiota differed between the most distant sites. The genus Citrobacter significantly differs between sites, which may indicate a difference in food availability, environmental microbiota, or both. Also, we identify the common core microbiome for Platysternon across Hong Kong as the shared taxa across the three sites. Additionally, beta diversity differs between host species. Since the two species are from the same site and encounter the same environmental microbiota, we infer that there is a host effect on the fecal microbiota, such as diet or the recruitment of host-adapted bacteria. Lastly, functional analyses found metabolism pathways (KEGG level 1) to be the most common, and pathways (KEGG level 3) to be statistically significant between sites, but statistically indistinguishable between species at the same site. Conclusions We find that fecal microbiota can significantly differ at a fine geographic scale and between syntopic hosts. Also, the function of fecal microbiota seems to be strongly affected by geographic site, rather than species. This study characterizes the identity and function of the fecal microbiota of two endangered turtle species, from what is likely their last remaining wild populations. These data of healthy, wild fecal microbiota will serve as a baseline for comparison and contribute to the conservation of these two endangered species.https://doi.org/10.1186/s12866-024-03227-2Gut microbiomeCommon core microbiomeTurtle conservationCitrobacter |
| spellingShingle | Jonathan J. Fong Yik-Hei Sung Li Ding Fine-scale geographic difference of the endangered Big-headed Turtle (Platysternon megacephalum) fecal microbiota, and comparison with the syntopic Beale’s Eyed Turtle (Sacalia bealei) BMC Microbiology Gut microbiome Common core microbiome Turtle conservation Citrobacter |
| title | Fine-scale geographic difference of the endangered Big-headed Turtle (Platysternon megacephalum) fecal microbiota, and comparison with the syntopic Beale’s Eyed Turtle (Sacalia bealei) |
| title_full | Fine-scale geographic difference of the endangered Big-headed Turtle (Platysternon megacephalum) fecal microbiota, and comparison with the syntopic Beale’s Eyed Turtle (Sacalia bealei) |
| title_fullStr | Fine-scale geographic difference of the endangered Big-headed Turtle (Platysternon megacephalum) fecal microbiota, and comparison with the syntopic Beale’s Eyed Turtle (Sacalia bealei) |
| title_full_unstemmed | Fine-scale geographic difference of the endangered Big-headed Turtle (Platysternon megacephalum) fecal microbiota, and comparison with the syntopic Beale’s Eyed Turtle (Sacalia bealei) |
| title_short | Fine-scale geographic difference of the endangered Big-headed Turtle (Platysternon megacephalum) fecal microbiota, and comparison with the syntopic Beale’s Eyed Turtle (Sacalia bealei) |
| title_sort | fine scale geographic difference of the endangered big headed turtle platysternon megacephalum fecal microbiota and comparison with the syntopic beale s eyed turtle sacalia bealei |
| topic | Gut microbiome Common core microbiome Turtle conservation Citrobacter |
| url | https://doi.org/10.1186/s12866-024-03227-2 |
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