Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters
Analogues of α-ketoglutarate are used in many cellular studies but assumptions are made about cellular uptake. Here, the authors show that esterified analogues rapidly hydrolyse in aqueous medium resulting in an analogue which can be quickly taken up by many cell lines, contrary to prevailing assump...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-25228-9 |
| _version_ | 1818570445096484864 |
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| author | Seth J. Parker Joel Encarnación-Rosado Kate E. R. Hollinshead David M. Hollinshead Leonard J. Ash Juan A. K. Rossi Elaine Y. Lin Albert S. W. Sohn Mark R. Philips Drew R. Jones Alec C. Kimmelman |
| author_facet | Seth J. Parker Joel Encarnación-Rosado Kate E. R. Hollinshead David M. Hollinshead Leonard J. Ash Juan A. K. Rossi Elaine Y. Lin Albert S. W. Sohn Mark R. Philips Drew R. Jones Alec C. Kimmelman |
| author_sort | Seth J. Parker |
| collection | DOAJ |
| description | Analogues of α-ketoglutarate are used in many cellular studies but assumptions are made about cellular uptake. Here, the authors show that esterified analogues rapidly hydrolyse in aqueous medium resulting in an analogue which can be quickly taken up by many cell lines, contrary to prevailing assumptions. |
| first_indexed | 2024-12-14T13:41:24Z |
| format | Article |
| id | doaj.art-a430c84ac3014555b5b20af966982f02 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-14T13:41:24Z |
| publishDate | 2021-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-a430c84ac3014555b5b20af966982f022022-12-21T22:59:26ZengNature PortfolioNature Communications2041-17232021-08-0112111510.1038/s41467-021-25228-9Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate estersSeth J. Parker0Joel Encarnación-Rosado1Kate E. R. Hollinshead2David M. Hollinshead3Leonard J. Ash4Juan A. K. Rossi5Elaine Y. Lin6Albert S. W. Sohn7Mark R. Philips8Drew R. Jones9Alec C. Kimmelman10Department of Radiation Oncology, New York University School of MedicineDepartment of Radiation Oncology, New York University School of MedicineDepartment of Radiation Oncology, New York University School of MedicineElixir Software Ltd., MacclesfieldDivision of Advanced Research Technologies, New York University School of MedicinePerlmutter Cancer Center, New York University School of MedicineDepartment of Radiation Oncology, New York University School of MedicineDepartment of Radiation Oncology, New York University School of MedicinePerlmutter Cancer Center, New York University School of MedicineDivision of Advanced Research Technologies, New York University School of MedicineDepartment of Radiation Oncology, New York University School of MedicineAnalogues of α-ketoglutarate are used in many cellular studies but assumptions are made about cellular uptake. Here, the authors show that esterified analogues rapidly hydrolyse in aqueous medium resulting in an analogue which can be quickly taken up by many cell lines, contrary to prevailing assumptions.https://doi.org/10.1038/s41467-021-25228-9 |
| spellingShingle | Seth J. Parker Joel Encarnación-Rosado Kate E. R. Hollinshead David M. Hollinshead Leonard J. Ash Juan A. K. Rossi Elaine Y. Lin Albert S. W. Sohn Mark R. Philips Drew R. Jones Alec C. Kimmelman Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters Nature Communications |
| title | Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters |
| title_full | Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters |
| title_fullStr | Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters |
| title_full_unstemmed | Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters |
| title_short | Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters |
| title_sort | spontaneous hydrolysis and spurious metabolic properties of α ketoglutarate esters |
| url | https://doi.org/10.1038/s41467-021-25228-9 |
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