Refining Stereotaxic Neurosurgery Techniques and Welfare Assessment for Long-Term Intracerebroventricular Device Implantation in Rodents
Stereotaxic surgeries enable precise access to specific brain regions, being of particular interest for chronic intracerebroventricular drug delivery. However, the challenge of long-term studies at this level is to allow the implantation of drug storage devices and their correct intrathecal connecti...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/16/2627 |
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| author | Ester Pérez-Martín Almudena Coto-Vilcapoma Juan Castilla-Silgado María Rodríguez-Cañón Catuxa Prado Gabriel Álvarez Marco Antonio Álvarez-Vega Benjamín Fernández-García Manuel Menéndez-González Cristina Tomás-Zapico |
| author_facet | Ester Pérez-Martín Almudena Coto-Vilcapoma Juan Castilla-Silgado María Rodríguez-Cañón Catuxa Prado Gabriel Álvarez Marco Antonio Álvarez-Vega Benjamín Fernández-García Manuel Menéndez-González Cristina Tomás-Zapico |
| author_sort | Ester Pérez-Martín |
| collection | DOAJ |
| description | Stereotaxic surgeries enable precise access to specific brain regions, being of particular interest for chronic intracerebroventricular drug delivery. However, the challenge of long-term studies at this level is to allow the implantation of drug storage devices and their correct intrathecal connection while guaranteeing animal welfare during the entire study period. In this study, we propose an optimized method for safe intrathecal device implantation, focusing on preoperative, intraoperative, and postoperative procedures, following the 3Rs principle and animal welfare regulations. Our optimized protocol introduces three main refinements. Firstly, we modify the dimensions of the implantable devices, notably diminishing the device-to-mouse weight ratio. Secondly, we use a combination of cyanoacrylate tissue adhesive and UV light-curing resin, which decreases surgery time, improves healing, and notably minimizes cannula detachment or adverse effects. Thirdly, we develop a customized welfare assessment scoresheet to accurately monitor animal well-being during long-term implantations. Taken together, these refinements positively impacted animal welfare by minimizing the negative effects on body weight, surgery-related complications, and anxiety-like behaviors. Overall, the proposed refinements have the potential to reduce animal use, enhance experimental data quality, and improve reproducibility. Additionally, these improvements can be extended to other neurosurgical techniques, thereby advancing neuroscience research, and benefiting the scientific community. |
| first_indexed | 2024-03-11T00:11:44Z |
| format | Article |
| id | doaj.art-a438ada869d44a6e8d3251d587e9451b |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T00:11:44Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-a438ada869d44a6e8d3251d587e9451b2023-11-18T23:57:53ZengMDPI AGAnimals2076-26152023-08-011316262710.3390/ani13162627Refining Stereotaxic Neurosurgery Techniques and Welfare Assessment for Long-Term Intracerebroventricular Device Implantation in RodentsEster Pérez-Martín0Almudena Coto-Vilcapoma1Juan Castilla-Silgado2María Rodríguez-Cañón3Catuxa Prado4Gabriel Álvarez5Marco Antonio Álvarez-Vega6Benjamín Fernández-García7Manuel Menéndez-González8Cristina Tomás-Zapico9Neuroscience Innovative Technologies S.L., Neurostech, 33428 Llanera, SpainDepartamento de Biología Funcional, Área de Fisiología, Universidad de Oviedo, 33006 Oviedo, SpainDepartamento de Biología Funcional, Área de Fisiología, Universidad de Oviedo, 33006 Oviedo, SpainNeuroscience Innovative Technologies S.L., Neurostech, 33428 Llanera, SpainNeuroscience Innovative Technologies S.L., Neurostech, 33428 Llanera, SpainNeuroscience Innovative Technologies S.L., Neurostech, 33428 Llanera, SpainDepartamento de Cirugía, Área de Cirugía, Universidad de Oviedo, 33006 Oviedo, SpainDepartamento de Biología Funcional, Área de Fisiología, Universidad de Oviedo, 33006 Oviedo, SpainInstituto de Investigación Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, SpainDepartamento de Biología Funcional, Área de Fisiología, Universidad de Oviedo, 33006 Oviedo, SpainStereotaxic surgeries enable precise access to specific brain regions, being of particular interest for chronic intracerebroventricular drug delivery. However, the challenge of long-term studies at this level is to allow the implantation of drug storage devices and their correct intrathecal connection while guaranteeing animal welfare during the entire study period. In this study, we propose an optimized method for safe intrathecal device implantation, focusing on preoperative, intraoperative, and postoperative procedures, following the 3Rs principle and animal welfare regulations. Our optimized protocol introduces three main refinements. Firstly, we modify the dimensions of the implantable devices, notably diminishing the device-to-mouse weight ratio. Secondly, we use a combination of cyanoacrylate tissue adhesive and UV light-curing resin, which decreases surgery time, improves healing, and notably minimizes cannula detachment or adverse effects. Thirdly, we develop a customized welfare assessment scoresheet to accurately monitor animal well-being during long-term implantations. Taken together, these refinements positively impacted animal welfare by minimizing the negative effects on body weight, surgery-related complications, and anxiety-like behaviors. Overall, the proposed refinements have the potential to reduce animal use, enhance experimental data quality, and improve reproducibility. Additionally, these improvements can be extended to other neurosurgical techniques, thereby advancing neuroscience research, and benefiting the scientific community.https://www.mdpi.com/2076-2615/13/16/26273Rsanimal researchanimal welfarecannula fixationintrathecal implantationpreclinical neurosurgery |
| spellingShingle | Ester Pérez-Martín Almudena Coto-Vilcapoma Juan Castilla-Silgado María Rodríguez-Cañón Catuxa Prado Gabriel Álvarez Marco Antonio Álvarez-Vega Benjamín Fernández-García Manuel Menéndez-González Cristina Tomás-Zapico Refining Stereotaxic Neurosurgery Techniques and Welfare Assessment for Long-Term Intracerebroventricular Device Implantation in Rodents Animals 3Rs animal research animal welfare cannula fixation intrathecal implantation preclinical neurosurgery |
| title | Refining Stereotaxic Neurosurgery Techniques and Welfare Assessment for Long-Term Intracerebroventricular Device Implantation in Rodents |
| title_full | Refining Stereotaxic Neurosurgery Techniques and Welfare Assessment for Long-Term Intracerebroventricular Device Implantation in Rodents |
| title_fullStr | Refining Stereotaxic Neurosurgery Techniques and Welfare Assessment for Long-Term Intracerebroventricular Device Implantation in Rodents |
| title_full_unstemmed | Refining Stereotaxic Neurosurgery Techniques and Welfare Assessment for Long-Term Intracerebroventricular Device Implantation in Rodents |
| title_short | Refining Stereotaxic Neurosurgery Techniques and Welfare Assessment for Long-Term Intracerebroventricular Device Implantation in Rodents |
| title_sort | refining stereotaxic neurosurgery techniques and welfare assessment for long term intracerebroventricular device implantation in rodents |
| topic | 3Rs animal research animal welfare cannula fixation intrathecal implantation preclinical neurosurgery |
| url | https://www.mdpi.com/2076-2615/13/16/2627 |
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