Antitumor and anti-angiogenic potentials of isolated crude saponins and various fractions of Rumexhastatus D. Don
BACKGROUND: Cancer, being the foremost challenge of the modern era and the focus of world-class investigators, gargantuan research is in progress worldwide to explore novel therapeutic for its management. The exploitation of natural sources has been proven to be an excellent approach to treat or min...
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| Format: | Article |
| Language: | English |
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BMC
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100018&lng=en&tlng=en |
| _version_ | 1811198455828185088 |
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| author | Sajjad Ahmad Farhat Ullah Muhammad Ayaz Anwar Zeb Farman Ullah Abdul Sadiq |
| author_facet | Sajjad Ahmad Farhat Ullah Muhammad Ayaz Anwar Zeb Farman Ullah Abdul Sadiq |
| author_sort | Sajjad Ahmad |
| collection | DOAJ |
| description | BACKGROUND: Cancer, being the foremost challenge of the modern era and the focus of world-class investigators, gargantuan research is in progress worldwide to explore novel therapeutic for its management. The exploitation of natural sources has been proven to be an excellent approach to treat or minify the excessive angiogenesis and proliferation of cells. Similarly, based the ethnomedicinal uses and literature survey, the current study is designed to explore the anti-tumor and anti-angiogenic potentials of Rumex hastatus. Anti-tumor and anti-angiogenic activities were carried out using potato-disc model and chorioallantoic membrane (CAM) assay respectively. Moreover, R. hastatus was also assessed for antibacterial activity against Agrobacterium tumefaciens (tumor causing bacterial strain). The positive controls used in anti-tumor, anti-angiogenic and antibacterial activities were vincristine sulphate, dexamethasone and cefotaxime respectively RESULTS: The crude saponins (Rh.Sp), methanolic extract (Rh.Cr) and other solvent extracts like n-hexane (Rh.Hex), chloroform (Rh.Chf), ethylacetate (Rh.EtAc) and aqueous fraction (Rh.Aq) exhibited notable anti-tumor and anti-angiogenic activities. In potato tumor assay, the chloroform and saponin fractions were observed to be the most effective showing 86.7 and 93.3 % tumor inhibition at 1000 μg/ml with IC50 values 31.6 and 18.1 μg/ml respectively. Similarly, these two samples i.e., chloroform and saponins also excelled among the entire test samples in anti-angiogenic evaluation exhibiting 81.6 % (IC50 = 17.9 μg/ml) and 78.9 % (IC50 = 64.9 μg/ml) at 1000 μg/ml respectively. In contrast, the antibacterial investigations revealed a negligible potential against A. tumefaciens. CONCLUSION: Based on our results we can claim that R. hastatus possesses both anti-tumor and anti-angiogenic potentials. In all of the solvent fractions, Rh.Chf and Rh.Sp were most effective against tumor and angiogenesis while having negligible activity against A. tumefaciens. It can be concluded that Rh.Chf and Rh.Sp might be potential targets in the isolation of natural product having anti-neoplastic action. |
| first_indexed | 2024-04-12T01:32:05Z |
| format | Article |
| id | doaj.art-a43b9507a6be4b7887892fe0bc394270 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 |
| language | English |
| last_indexed | 2024-04-12T01:32:05Z |
| publisher | BMC |
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| series | Biological Research |
| spelling | doaj.art-a43b9507a6be4b7887892fe0bc3942702022-12-22T03:53:27ZengBMCBiological Research0716-97604901910.1186/s40659-016-0079-2S0716-97602016000100018Antitumor and anti-angiogenic potentials of isolated crude saponins and various fractions of Rumexhastatus D. DonSajjad Ahmad0Farhat Ullah1Muhammad Ayaz2Anwar Zeb3Farman Ullah4Abdul Sadiq5University of MalakandUniversity of MalakandUniversity of MalakandUniversity of MalakandKohat University of Science and TechnologyUniversity of MalakandBACKGROUND: Cancer, being the foremost challenge of the modern era and the focus of world-class investigators, gargantuan research is in progress worldwide to explore novel therapeutic for its management. The exploitation of natural sources has been proven to be an excellent approach to treat or minify the excessive angiogenesis and proliferation of cells. Similarly, based the ethnomedicinal uses and literature survey, the current study is designed to explore the anti-tumor and anti-angiogenic potentials of Rumex hastatus. Anti-tumor and anti-angiogenic activities were carried out using potato-disc model and chorioallantoic membrane (CAM) assay respectively. Moreover, R. hastatus was also assessed for antibacterial activity against Agrobacterium tumefaciens (tumor causing bacterial strain). The positive controls used in anti-tumor, anti-angiogenic and antibacterial activities were vincristine sulphate, dexamethasone and cefotaxime respectively RESULTS: The crude saponins (Rh.Sp), methanolic extract (Rh.Cr) and other solvent extracts like n-hexane (Rh.Hex), chloroform (Rh.Chf), ethylacetate (Rh.EtAc) and aqueous fraction (Rh.Aq) exhibited notable anti-tumor and anti-angiogenic activities. In potato tumor assay, the chloroform and saponin fractions were observed to be the most effective showing 86.7 and 93.3 % tumor inhibition at 1000 μg/ml with IC50 values 31.6 and 18.1 μg/ml respectively. Similarly, these two samples i.e., chloroform and saponins also excelled among the entire test samples in anti-angiogenic evaluation exhibiting 81.6 % (IC50 = 17.9 μg/ml) and 78.9 % (IC50 = 64.9 μg/ml) at 1000 μg/ml respectively. In contrast, the antibacterial investigations revealed a negligible potential against A. tumefaciens. CONCLUSION: Based on our results we can claim that R. hastatus possesses both anti-tumor and anti-angiogenic potentials. In all of the solvent fractions, Rh.Chf and Rh.Sp were most effective against tumor and angiogenesis while having negligible activity against A. tumefaciens. It can be concluded that Rh.Chf and Rh.Sp might be potential targets in the isolation of natural product having anti-neoplastic action.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100018&lng=en&tlng=enRumex hastatusAgrobacterium tumefaciensSaponinsAngiogenesisTumor |
| spellingShingle | Sajjad Ahmad Farhat Ullah Muhammad Ayaz Anwar Zeb Farman Ullah Abdul Sadiq Antitumor and anti-angiogenic potentials of isolated crude saponins and various fractions of Rumexhastatus D. Don Biological Research Rumex hastatus Agrobacterium tumefaciens Saponins Angiogenesis Tumor |
| title | Antitumor and anti-angiogenic potentials of isolated crude saponins and various fractions of Rumexhastatus D. Don |
| title_full | Antitumor and anti-angiogenic potentials of isolated crude saponins and various fractions of Rumexhastatus D. Don |
| title_fullStr | Antitumor and anti-angiogenic potentials of isolated crude saponins and various fractions of Rumexhastatus D. Don |
| title_full_unstemmed | Antitumor and anti-angiogenic potentials of isolated crude saponins and various fractions of Rumexhastatus D. Don |
| title_short | Antitumor and anti-angiogenic potentials of isolated crude saponins and various fractions of Rumexhastatus D. Don |
| title_sort | antitumor and anti angiogenic potentials of isolated crude saponins and various fractions of rumexhastatus d don |
| topic | Rumex hastatus Agrobacterium tumefaciens Saponins Angiogenesis Tumor |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100018&lng=en&tlng=en |
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