Cytokine Profiling of Amniotic Fluid from Congenital Cytomegalovirus Infection
Background: Congenital cytomegalovirus (cCMV) infection is frequent and potentially severe. The immunobiology of cCMV infection is poorly understood, involving cytokines that could be carried within or on the surface of extracellular vesicles (EV). We investigated intra-amniotic cytokines, mediated...
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MDPI AG
2022-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/10/2145 |
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| author | Nicolas Bourgon Wendy Fitzgerald Hugues Aschard Jean-François Magny Tiffany Guilleminot Julien Stirnemann Roberto Romero Yves Ville Leonid Margolis Marianne Leruez-Ville |
| author_facet | Nicolas Bourgon Wendy Fitzgerald Hugues Aschard Jean-François Magny Tiffany Guilleminot Julien Stirnemann Roberto Romero Yves Ville Leonid Margolis Marianne Leruez-Ville |
| author_sort | Nicolas Bourgon |
| collection | DOAJ |
| description | Background: Congenital cytomegalovirus (cCMV) infection is frequent and potentially severe. The immunobiology of cCMV infection is poorly understood, involving cytokines that could be carried within or on the surface of extracellular vesicles (EV). We investigated intra-amniotic cytokines, mediated or not by EV, in cCMV infection. Methods: Forty infected fetuses following early maternal primary infection and forty negative controls were included. Infected fetuses were classified according to severity at birth: asymptomatic, moderately or severely symptomatic. Following the capture of EV in amniotic fluid (AF), the concentrations of 38 cytokines were quantified. The association with infection and its severity was determined using univariate and multivariate analysis. A prediction analysis based on principal component analysis was conducted. Results: cCMV infection was nominally associated with an increase in six cytokines, mainly soluble (IP-10, IL-18, ITAC, and TRAIL). EV-associated IP-10 was also increased in cases of fetal infection. Severity of fetal infection was nominally associated with an increase in twelve cytokines, including five also associated with fetal infection. A pattern of specific increase in six proteins fitted severely symptomatic infection, including IL-18soluble, TRAILsoluble, CRPsoluble, TRAILsurface, MIGinternal, and RANTESinternal. Conclusion: Fetal infection and its severity are associated with an increase in pro-inflammatory cytokines involved in Th1 immune response. |
| first_indexed | 2024-03-09T19:24:09Z |
| format | Article |
| id | doaj.art-a43c406b08af47358b8fc065cf48cac1 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T19:24:09Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-a43c406b08af47358b8fc065cf48cac12023-11-24T03:08:14ZengMDPI AGViruses1999-49152022-09-011410214510.3390/v14102145Cytokine Profiling of Amniotic Fluid from Congenital Cytomegalovirus InfectionNicolas Bourgon0Wendy Fitzgerald1Hugues Aschard2Jean-François Magny3Tiffany Guilleminot4Julien Stirnemann5Roberto Romero6Yves Ville7Leonid Margolis8Marianne Leruez-Ville9Service d’Obstétrique—Maternité, Chirurgie, Médecine et Imagerie Fœtales, Hôpital Necker Enfants Malades, GHU Paris Centre, AP-HP, F-75015 Paris, FranceSection of Intercellular Interactions, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, MD 20892, USADepartment of Computational Biology, Institut Pasteur, Université Paris Cité, F-75015 Paris, FranceÉquipe d’Accueil «FŒTUS» 73-28, Université Paris Cité, F-75015 Paris, FranceÉquipe d’Accueil «FŒTUS» 73-28, Université Paris Cité, F-75015 Paris, FranceService d’Obstétrique—Maternité, Chirurgie, Médecine et Imagerie Fœtales, Hôpital Necker Enfants Malades, GHU Paris Centre, AP-HP, F-75015 Paris, FrancePerinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, MD 20892, USAService d’Obstétrique—Maternité, Chirurgie, Médecine et Imagerie Fœtales, Hôpital Necker Enfants Malades, GHU Paris Centre, AP-HP, F-75015 Paris, FranceSection of Intercellular Interactions, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, MD 20892, USAÉquipe d’Accueil «FŒTUS» 73-28, Université Paris Cité, F-75015 Paris, FranceBackground: Congenital cytomegalovirus (cCMV) infection is frequent and potentially severe. The immunobiology of cCMV infection is poorly understood, involving cytokines that could be carried within or on the surface of extracellular vesicles (EV). We investigated intra-amniotic cytokines, mediated or not by EV, in cCMV infection. Methods: Forty infected fetuses following early maternal primary infection and forty negative controls were included. Infected fetuses were classified according to severity at birth: asymptomatic, moderately or severely symptomatic. Following the capture of EV in amniotic fluid (AF), the concentrations of 38 cytokines were quantified. The association with infection and its severity was determined using univariate and multivariate analysis. A prediction analysis based on principal component analysis was conducted. Results: cCMV infection was nominally associated with an increase in six cytokines, mainly soluble (IP-10, IL-18, ITAC, and TRAIL). EV-associated IP-10 was also increased in cases of fetal infection. Severity of fetal infection was nominally associated with an increase in twelve cytokines, including five also associated with fetal infection. A pattern of specific increase in six proteins fitted severely symptomatic infection, including IL-18soluble, TRAILsoluble, CRPsoluble, TRAILsurface, MIGinternal, and RANTESinternal. Conclusion: Fetal infection and its severity are associated with an increase in pro-inflammatory cytokines involved in Th1 immune response.https://www.mdpi.com/1999-4915/14/10/2145congenital cytomegalovirus infectioncytokinesextracellular vesiclesamniotic fluid |
| spellingShingle | Nicolas Bourgon Wendy Fitzgerald Hugues Aschard Jean-François Magny Tiffany Guilleminot Julien Stirnemann Roberto Romero Yves Ville Leonid Margolis Marianne Leruez-Ville Cytokine Profiling of Amniotic Fluid from Congenital Cytomegalovirus Infection Viruses congenital cytomegalovirus infection cytokines extracellular vesicles amniotic fluid |
| title | Cytokine Profiling of Amniotic Fluid from Congenital Cytomegalovirus Infection |
| title_full | Cytokine Profiling of Amniotic Fluid from Congenital Cytomegalovirus Infection |
| title_fullStr | Cytokine Profiling of Amniotic Fluid from Congenital Cytomegalovirus Infection |
| title_full_unstemmed | Cytokine Profiling of Amniotic Fluid from Congenital Cytomegalovirus Infection |
| title_short | Cytokine Profiling of Amniotic Fluid from Congenital Cytomegalovirus Infection |
| title_sort | cytokine profiling of amniotic fluid from congenital cytomegalovirus infection |
| topic | congenital cytomegalovirus infection cytokines extracellular vesicles amniotic fluid |
| url | https://www.mdpi.com/1999-4915/14/10/2145 |
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