Dynamic motile T cells highly respond to the T cell stimulation via PI3K-Akt and NF-κB pathways.
T lymphocytes (T cells) circulate from the blood into secondary lymphoid organs for immune surveillance. In this study, we hypothesized that circulating T cells are heterogeneous and can be grouped according to their differential migratory capacity in response to chemoattractants, rather than expres...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3608537?pdf=render |
| _version_ | 1811212367020687360 |
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| author | Hye-Ran Kim Bo-Ra Na Min-Sung Kwon Yoo-Seung Ko Weon-Cheol Han Chang-Duk Jun |
| author_facet | Hye-Ran Kim Bo-Ra Na Min-Sung Kwon Yoo-Seung Ko Weon-Cheol Han Chang-Duk Jun |
| author_sort | Hye-Ran Kim |
| collection | DOAJ |
| description | T lymphocytes (T cells) circulate from the blood into secondary lymphoid organs for immune surveillance. In this study, we hypothesized that circulating T cells are heterogeneous and can be grouped according to their differential migratory capacity in response to chemoattractants, rather than expressions of certain receptors or cytokines. We further hypothesized that, at least in part, this intrinsic difference in motility may be related to the T cell function. We established motile (m-T) and non-motile T (nm-T) cell lines based on their response to the chemokine SDF-1α. m-T cells showed more irregular and polarized morphologies than nm-T cells did. Interestingly, m-T cells produced higher levels of IL-2, a marker for T cell activation, than nm-T cells did after stimulation; however, no differences were observed in terms of surface expression of T cell receptors (TCR), adhesion molecules LFA-1 and ICAM-1, and chemokine receptor CXCR4. Both cell lines also showed similar membrane events (i.e., T cell-APC conjugation, LFA-1 accumulation at the immunological synapse, and TCR internalization). In contrast, PKC-θ, a downstream of PI3K-Akt pathway was constitutively activated in m-T cells and the activation was more prominent during T cell stimulation. Consequently, NF-κB activity was selectively upregulated in m-T cells. This study is the first, to our knowledge, to demonstrate that T cells can be subcategorized on the basis of their intrinsic migratory capacity in relation to T cell activation. |
| first_indexed | 2024-04-12T05:28:20Z |
| format | Article |
| id | doaj.art-a4410dc057f04496bd4bd393520ab9ec |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T05:28:20Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a4410dc057f04496bd4bd393520ab9ec2022-12-22T03:46:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5979310.1371/journal.pone.0059793Dynamic motile T cells highly respond to the T cell stimulation via PI3K-Akt and NF-κB pathways.Hye-Ran KimBo-Ra NaMin-Sung KwonYoo-Seung KoWeon-Cheol HanChang-Duk JunT lymphocytes (T cells) circulate from the blood into secondary lymphoid organs for immune surveillance. In this study, we hypothesized that circulating T cells are heterogeneous and can be grouped according to their differential migratory capacity in response to chemoattractants, rather than expressions of certain receptors or cytokines. We further hypothesized that, at least in part, this intrinsic difference in motility may be related to the T cell function. We established motile (m-T) and non-motile T (nm-T) cell lines based on their response to the chemokine SDF-1α. m-T cells showed more irregular and polarized morphologies than nm-T cells did. Interestingly, m-T cells produced higher levels of IL-2, a marker for T cell activation, than nm-T cells did after stimulation; however, no differences were observed in terms of surface expression of T cell receptors (TCR), adhesion molecules LFA-1 and ICAM-1, and chemokine receptor CXCR4. Both cell lines also showed similar membrane events (i.e., T cell-APC conjugation, LFA-1 accumulation at the immunological synapse, and TCR internalization). In contrast, PKC-θ, a downstream of PI3K-Akt pathway was constitutively activated in m-T cells and the activation was more prominent during T cell stimulation. Consequently, NF-κB activity was selectively upregulated in m-T cells. This study is the first, to our knowledge, to demonstrate that T cells can be subcategorized on the basis of their intrinsic migratory capacity in relation to T cell activation.http://europepmc.org/articles/PMC3608537?pdf=render |
| spellingShingle | Hye-Ran Kim Bo-Ra Na Min-Sung Kwon Yoo-Seung Ko Weon-Cheol Han Chang-Duk Jun Dynamic motile T cells highly respond to the T cell stimulation via PI3K-Akt and NF-κB pathways. PLoS ONE |
| title | Dynamic motile T cells highly respond to the T cell stimulation via PI3K-Akt and NF-κB pathways. |
| title_full | Dynamic motile T cells highly respond to the T cell stimulation via PI3K-Akt and NF-κB pathways. |
| title_fullStr | Dynamic motile T cells highly respond to the T cell stimulation via PI3K-Akt and NF-κB pathways. |
| title_full_unstemmed | Dynamic motile T cells highly respond to the T cell stimulation via PI3K-Akt and NF-κB pathways. |
| title_short | Dynamic motile T cells highly respond to the T cell stimulation via PI3K-Akt and NF-κB pathways. |
| title_sort | dynamic motile t cells highly respond to the t cell stimulation via pi3k akt and nf κb pathways |
| url | http://europepmc.org/articles/PMC3608537?pdf=render |
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