Effects of fermented Broussonetia papyrifera on growth, intestinal antioxidant, inflammation and microbiota of grass carp (Ctenopharyngodon idella)
The present study was conducted to investigate the influence of fermented Broussonetia Papyrifera (FBP) on growth performance, intestinal antioxidant capacity, inflammation and microbiota of grass carp. Fish (initial weight: 50.0 ± 0.5 g) were fed diets with 0 (control diet), 5 % (FBP5), 10 % (FBP10...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513421000892 |
| _version_ | 1818907485503750144 |
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| author | Tao Tang Fangle Tong Shengnan Zhao Jinhai Bai Zehong Wei Yi Hu Shaojun Liu |
| author_facet | Tao Tang Fangle Tong Shengnan Zhao Jinhai Bai Zehong Wei Yi Hu Shaojun Liu |
| author_sort | Tao Tang |
| collection | DOAJ |
| description | The present study was conducted to investigate the influence of fermented Broussonetia Papyrifera (FBP) on growth performance, intestinal antioxidant capacity, inflammation and microbiota of grass carp. Fish (initial weight: 50.0 ± 0.5 g) were fed diets with 0 (control diet), 5 % (FBP5), 10 % (FBP10), 15 % (FBP15) and 20 % (FBP20) of FBP supplementation respectively for 8 weeks. The results showed that FBP5 group significantly decreased FCR of grass carp (P < 0.05). Besides, FBP supplementation significantly decreased intestine Cu/Zn SOD activity (P < 0.05), as well as significantly elevated mRNA expression level of kelch-like-ECH-associated protein 1 (keap1) and depressed mRNA expression level of NF-E2-related factor 2 (nrf2), superoxide dismutase 1 (sod1) and catalase (cat) (P < 0.05). The content of malondialdehyde (MDA) increased significantly in the FBP20 group compared to the control diet (P < 0.05). The expression level of interleukin-8 (il-8) and interferon γ (ifnγ) downregulated with increased dietary FBP, and significant difference was found in FBP20 group (P < 0.05). In addition, when the supplementation of FBP was no more than 15 %, the abundance of Firmicutes and Fusobacteria increased significantly, while the abundance of Actinobacteria and Cyanobacteria decreased significantly in intestine of grass carp (P < 0.05). Compared to the control diet, the richness and diversity of intestinal microbiota of grass carp decreased significantly in FBP20 group (P < 0.05). According to the second order polynomial regression equation analysis of SGR and FCR, the optimal additional level of FBP was estimated to be 4.58 % and 5.28 %, respectively. The recommended maximum addition of FBP in diet of grass carp was up to 15 % according to the results of intestinal microbiota. |
| first_indexed | 2024-12-19T21:55:52Z |
| format | Article |
| id | doaj.art-a441cefbe6c1450ba213b5a0906bb455 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-19T21:55:52Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-a441cefbe6c1450ba213b5a0906bb4552022-12-21T20:04:17ZengElsevierAquaculture Reports2352-51342021-07-0120100673Effects of fermented Broussonetia papyrifera on growth, intestinal antioxidant, inflammation and microbiota of grass carp (Ctenopharyngodon idella)Tao Tang0Fangle Tong1Shengnan Zhao2Jinhai Bai3Zehong Wei4Yi Hu5Shaojun Liu6State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha, 410081, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha, 410081, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha, 410081, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha, 410081, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha, 410081, China; Corresponding author at: State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, 36 Lushan Road, Changsha, 410081, China.Hunan Engineering Research Center for Utilization of Characteristics of Aquatic Resources, Hunan Agricultural University, Changsha, 410128, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha, 410081, ChinaThe present study was conducted to investigate the influence of fermented Broussonetia Papyrifera (FBP) on growth performance, intestinal antioxidant capacity, inflammation and microbiota of grass carp. Fish (initial weight: 50.0 ± 0.5 g) were fed diets with 0 (control diet), 5 % (FBP5), 10 % (FBP10), 15 % (FBP15) and 20 % (FBP20) of FBP supplementation respectively for 8 weeks. The results showed that FBP5 group significantly decreased FCR of grass carp (P < 0.05). Besides, FBP supplementation significantly decreased intestine Cu/Zn SOD activity (P < 0.05), as well as significantly elevated mRNA expression level of kelch-like-ECH-associated protein 1 (keap1) and depressed mRNA expression level of NF-E2-related factor 2 (nrf2), superoxide dismutase 1 (sod1) and catalase (cat) (P < 0.05). The content of malondialdehyde (MDA) increased significantly in the FBP20 group compared to the control diet (P < 0.05). The expression level of interleukin-8 (il-8) and interferon γ (ifnγ) downregulated with increased dietary FBP, and significant difference was found in FBP20 group (P < 0.05). In addition, when the supplementation of FBP was no more than 15 %, the abundance of Firmicutes and Fusobacteria increased significantly, while the abundance of Actinobacteria and Cyanobacteria decreased significantly in intestine of grass carp (P < 0.05). Compared to the control diet, the richness and diversity of intestinal microbiota of grass carp decreased significantly in FBP20 group (P < 0.05). According to the second order polynomial regression equation analysis of SGR and FCR, the optimal additional level of FBP was estimated to be 4.58 % and 5.28 %, respectively. The recommended maximum addition of FBP in diet of grass carp was up to 15 % according to the results of intestinal microbiota.http://www.sciencedirect.com/science/article/pii/S2352513421000892Fermented Broussonetia PapyriferaAntioxidantInflammationMicrobiotaGrass carp |
| spellingShingle | Tao Tang Fangle Tong Shengnan Zhao Jinhai Bai Zehong Wei Yi Hu Shaojun Liu Effects of fermented Broussonetia papyrifera on growth, intestinal antioxidant, inflammation and microbiota of grass carp (Ctenopharyngodon idella) Aquaculture Reports Fermented Broussonetia Papyrifera Antioxidant Inflammation Microbiota Grass carp |
| title | Effects of fermented Broussonetia papyrifera on growth, intestinal antioxidant, inflammation and microbiota of grass carp (Ctenopharyngodon idella) |
| title_full | Effects of fermented Broussonetia papyrifera on growth, intestinal antioxidant, inflammation and microbiota of grass carp (Ctenopharyngodon idella) |
| title_fullStr | Effects of fermented Broussonetia papyrifera on growth, intestinal antioxidant, inflammation and microbiota of grass carp (Ctenopharyngodon idella) |
| title_full_unstemmed | Effects of fermented Broussonetia papyrifera on growth, intestinal antioxidant, inflammation and microbiota of grass carp (Ctenopharyngodon idella) |
| title_short | Effects of fermented Broussonetia papyrifera on growth, intestinal antioxidant, inflammation and microbiota of grass carp (Ctenopharyngodon idella) |
| title_sort | effects of fermented broussonetia papyrifera on growth intestinal antioxidant inflammation and microbiota of grass carp ctenopharyngodon idella |
| topic | Fermented Broussonetia Papyrifera Antioxidant Inflammation Microbiota Grass carp |
| url | http://www.sciencedirect.com/science/article/pii/S2352513421000892 |
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