A rapid-crosslinking antimicrobial hydrogel with enhanced antibacterial capabilities for improving wound healing
One of the main reasons impeding wound healing is wound infection caused by bacterial colonization with a continuous stage of inflammation. Traditional wound treatments like gauze are being replaced by tissue adhesives with strong wet tissue adhesion and biocompatibility. Herein, a fast-crosslinking...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-05-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1206211/full |
| _version_ | 1797815383895310336 |
|---|---|
| author | Xi Zhang Xi Zhang Wanxin Li Genying Wei Yuling Yan Ruitao He Yan Wang Daoyuan Chen Xiaofei Qin |
| author_facet | Xi Zhang Xi Zhang Wanxin Li Genying Wei Yuling Yan Ruitao He Yan Wang Daoyuan Chen Xiaofei Qin |
| author_sort | Xi Zhang |
| collection | DOAJ |
| description | One of the main reasons impeding wound healing is wound infection caused by bacterial colonization with a continuous stage of inflammation. Traditional wound treatments like gauze are being replaced by tissue adhesives with strong wet tissue adhesion and biocompatibility. Herein, a fast-crosslinking hydrogel is developed to achieve both strong antimicrobial properties and excellent biocompatibility. In this study, a simple and non-toxic composite hydrogel was prepared by the Schiff base reaction between the aldehyde group of 2,3,4-trihydroxybenzaldehyde (TBA) and the amino group of ε-Poly-L-lysine (EPL). Subsequently, a succession of experiments toward this new hydrogel including structure characterization, antimicrobial properties, cell experiment and wound healing were applied. The results of the experiments show that the EPL-TBA hydrogel not only exhibited excellent contact-active antimicrobial activities against Gram-negative bacteria Escherichia coli (E. coil) and Gram-positive Bacteria Staphylococcus aureus (S. aureus), but also inhibited the biofilm formation. More importantly, the EPL-TBA hydrogel promoted the wound healing with low cytotoxicity in vivo. These findings indicate that the EPL-TBA hydrogel has a promising use as a wound dressing in the bacterial infection prevention and wounds healing acceleration. |
| first_indexed | 2024-03-13T08:21:52Z |
| format | Article |
| id | doaj.art-a44281ce74344dff8c28160cdb67eec8 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-03-13T08:21:52Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-a44281ce74344dff8c28160cdb67eec82023-05-31T07:14:19ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-05-011410.3389/fphys.2023.12062111206211A rapid-crosslinking antimicrobial hydrogel with enhanced antibacterial capabilities for improving wound healingXi Zhang0Xi Zhang1Wanxin Li2Genying Wei3Yuling Yan4Ruitao He5Yan Wang6Daoyuan Chen7Xiaofei Qin8School of Biological Engineering, Zunyi Medical University, Zhuhai, Guangdong, ChinaDepartment of Clinical Medicine, The Fifth Clinical Institution, Zhuhai Campus of Zunyi Medical University, Zhuhai, Guangdong, ChinaSchool of Biological Engineering, Zunyi Medical University, Zhuhai, Guangdong, ChinaSchool of Biological Engineering, Zunyi Medical University, Zhuhai, Guangdong, ChinaDepartment of Clinical Medicine, The Fifth Clinical Institution, Zhuhai Campus of Zunyi Medical University, Zhuhai, Guangdong, ChinaDepartment of Clinical Medicine, The Fifth Clinical Institution, Zhuhai Campus of Zunyi Medical University, Zhuhai, Guangdong, ChinaSchool of Biological Engineering, Zunyi Medical University, Zhuhai, Guangdong, ChinaSchool of Biological Engineering, Zunyi Medical University, Zhuhai, Guangdong, ChinaSchool of Biological Engineering, Zunyi Medical University, Zhuhai, Guangdong, ChinaOne of the main reasons impeding wound healing is wound infection caused by bacterial colonization with a continuous stage of inflammation. Traditional wound treatments like gauze are being replaced by tissue adhesives with strong wet tissue adhesion and biocompatibility. Herein, a fast-crosslinking hydrogel is developed to achieve both strong antimicrobial properties and excellent biocompatibility. In this study, a simple and non-toxic composite hydrogel was prepared by the Schiff base reaction between the aldehyde group of 2,3,4-trihydroxybenzaldehyde (TBA) and the amino group of ε-Poly-L-lysine (EPL). Subsequently, a succession of experiments toward this new hydrogel including structure characterization, antimicrobial properties, cell experiment and wound healing were applied. The results of the experiments show that the EPL-TBA hydrogel not only exhibited excellent contact-active antimicrobial activities against Gram-negative bacteria Escherichia coli (E. coil) and Gram-positive Bacteria Staphylococcus aureus (S. aureus), but also inhibited the biofilm formation. More importantly, the EPL-TBA hydrogel promoted the wound healing with low cytotoxicity in vivo. These findings indicate that the EPL-TBA hydrogel has a promising use as a wound dressing in the bacterial infection prevention and wounds healing acceleration.https://www.frontiersin.org/articles/10.3389/fphys.2023.1206211/fullhydrogelrapid-crosslinkingantimicrobialanti-inflammatorywound healing |
| spellingShingle | Xi Zhang Xi Zhang Wanxin Li Genying Wei Yuling Yan Ruitao He Yan Wang Daoyuan Chen Xiaofei Qin A rapid-crosslinking antimicrobial hydrogel with enhanced antibacterial capabilities for improving wound healing Frontiers in Physiology hydrogel rapid-crosslinking antimicrobial anti-inflammatory wound healing |
| title | A rapid-crosslinking antimicrobial hydrogel with enhanced antibacterial capabilities for improving wound healing |
| title_full | A rapid-crosslinking antimicrobial hydrogel with enhanced antibacterial capabilities for improving wound healing |
| title_fullStr | A rapid-crosslinking antimicrobial hydrogel with enhanced antibacterial capabilities for improving wound healing |
| title_full_unstemmed | A rapid-crosslinking antimicrobial hydrogel with enhanced antibacterial capabilities for improving wound healing |
| title_short | A rapid-crosslinking antimicrobial hydrogel with enhanced antibacterial capabilities for improving wound healing |
| title_sort | rapid crosslinking antimicrobial hydrogel with enhanced antibacterial capabilities for improving wound healing |
| topic | hydrogel rapid-crosslinking antimicrobial anti-inflammatory wound healing |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1206211/full |
| work_keys_str_mv | AT xizhang arapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT xizhang arapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT wanxinli arapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT genyingwei arapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT yulingyan arapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT ruitaohe arapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT yanwang arapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT daoyuanchen arapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT xiaofeiqin arapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT xizhang rapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT xizhang rapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT wanxinli rapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT genyingwei rapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT yulingyan rapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT ruitaohe rapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT yanwang rapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT daoyuanchen rapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing AT xiaofeiqin rapidcrosslinkingantimicrobialhydrogelwithenhancedantibacterialcapabilitiesforimprovingwoundhealing |