The Role of Crocetin-Loaded PLGA Nanoparticles as a Pre-Treatment Agent on Indocyanine-Photodynamic Therapy of Breast Cancer Cell
Introduction: Photodynamic therapy (PDT) can be considered as a non-invasive method for cancer treatment. One of the most commonly of a water-soluble dye photosensitizer (PS) used in photothermal therapy (PTT) and PDT is Indocyanine Green (ICG). However, high cytotoxicity in high concentration and...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2022-01-01
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| Series: | Iranian Journal of Medical Physics |
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| Online Access: | https://ijmp.mums.ac.ir/article_17845_325ecc903feabcb15df406ddf5acd96e.pdf |
| _version_ | 1797209639521091584 |
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| author | Samaneh Soudmand Salarabadi Maryam Hashemi Ameneh Sazgarnia |
| author_facet | Samaneh Soudmand Salarabadi Maryam Hashemi Ameneh Sazgarnia |
| author_sort | Samaneh Soudmand Salarabadi |
| collection | DOAJ |
| description | Introduction: Photodynamic therapy (PDT) can be considered as a non-invasive method for cancer treatment. One of the most commonly of a water-soluble dye photosensitizer (PS) used in photothermal therapy (PTT) and PDT is Indocyanine Green (ICG). However, high cytotoxicity in high concentration and instability in aqueous media were limited its application. It was shown that using nanoparticles or plant extracts in combination with PS could improve PDT efficiency. In this study, anti-cancer properties of crocetin (Crt) loaded PLGA (Poly lactic-co-glycolic acid) nanoparticles (NPs) were utilized to increase the PDT efficacy with ICG on the MCF-7 cells.
Material and Methods: Crt was encapsulated into PLGA NPs and its particle size distribution and encapsulation efficiency were evaluated. IC10 of Crt, PLGA-Crt NPs and ICG was determined by MTT assay in MCF-7 cancer cells. At these concentrations, the cells were pre-treated with Crt or PLG-Crt, then treated with ICG and finally exposure to near infrared (NIR) laser with 2.5 W powers at different times. The cells viability was evaluated by the MTT assay.
Results: The findings showed no dark cytotoxicity due to ICG (12.9 μM), Crt or PLGA-Crt alone. But NIR laser irradiation in the presence of ICG after cells pre-treatment by the Crt or PLGA-Crt NPs leads to induce cell death to (61.6 ±7) % and (75.5 ±5) %, respectively (P<0.05).
Conclusion: The results demonstrated that PLGA-Crt NPs in combination with ICG could improve PDT outcomes more efficiently in comparison with Crt and ICG. Therefore, this method could be effective in breast cancer therapy with low cytotoxicity. |
| first_indexed | 2024-04-24T09:57:54Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2345-3672 |
| language | English |
| last_indexed | 2024-04-24T09:57:54Z |
| publishDate | 2022-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Medical Physics |
| spelling | doaj.art-a4450ee2d2a448ff82ae2393e80502e42024-04-14T06:28:28ZengMashhad University of Medical SciencesIranian Journal of Medical Physics2345-36722022-01-01191586510.22038/ijmp.2021.56373.194217845The Role of Crocetin-Loaded PLGA Nanoparticles as a Pre-Treatment Agent on Indocyanine-Photodynamic Therapy of Breast Cancer CellSamaneh Soudmand Salarabadi0Maryam Hashemi1Ameneh Sazgarnia2Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, IranNanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences ,Mashhad, IranMedical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, IranIntroduction: Photodynamic therapy (PDT) can be considered as a non-invasive method for cancer treatment. One of the most commonly of a water-soluble dye photosensitizer (PS) used in photothermal therapy (PTT) and PDT is Indocyanine Green (ICG). However, high cytotoxicity in high concentration and instability in aqueous media were limited its application. It was shown that using nanoparticles or plant extracts in combination with PS could improve PDT efficiency. In this study, anti-cancer properties of crocetin (Crt) loaded PLGA (Poly lactic-co-glycolic acid) nanoparticles (NPs) were utilized to increase the PDT efficacy with ICG on the MCF-7 cells. Material and Methods: Crt was encapsulated into PLGA NPs and its particle size distribution and encapsulation efficiency were evaluated. IC10 of Crt, PLGA-Crt NPs and ICG was determined by MTT assay in MCF-7 cancer cells. At these concentrations, the cells were pre-treated with Crt or PLG-Crt, then treated with ICG and finally exposure to near infrared (NIR) laser with 2.5 W powers at different times. The cells viability was evaluated by the MTT assay. Results: The findings showed no dark cytotoxicity due to ICG (12.9 μM), Crt or PLGA-Crt alone. But NIR laser irradiation in the presence of ICG after cells pre-treatment by the Crt or PLGA-Crt NPs leads to induce cell death to (61.6 ±7) % and (75.5 ±5) %, respectively (P<0.05). Conclusion: The results demonstrated that PLGA-Crt NPs in combination with ICG could improve PDT outcomes more efficiently in comparison with Crt and ICG. Therefore, this method could be effective in breast cancer therapy with low cytotoxicity.https://ijmp.mums.ac.ir/article_17845_325ecc903feabcb15df406ddf5acd96e.pdfcrocetin (crt) poly lacticcoglycolic acid (plga) nanoparticles (nps) photodynamic therapy (pdt) indocyanine green (icg) breast cancer |
| spellingShingle | Samaneh Soudmand Salarabadi Maryam Hashemi Ameneh Sazgarnia The Role of Crocetin-Loaded PLGA Nanoparticles as a Pre-Treatment Agent on Indocyanine-Photodynamic Therapy of Breast Cancer Cell Iranian Journal of Medical Physics crocetin (crt) poly lactic co glycolic acid (plga) nanoparticles (nps) photodynamic therapy (pdt) indocyanine green (icg) breast cancer |
| title | The Role of Crocetin-Loaded PLGA Nanoparticles as a Pre-Treatment Agent on Indocyanine-Photodynamic Therapy of Breast Cancer Cell |
| title_full | The Role of Crocetin-Loaded PLGA Nanoparticles as a Pre-Treatment Agent on Indocyanine-Photodynamic Therapy of Breast Cancer Cell |
| title_fullStr | The Role of Crocetin-Loaded PLGA Nanoparticles as a Pre-Treatment Agent on Indocyanine-Photodynamic Therapy of Breast Cancer Cell |
| title_full_unstemmed | The Role of Crocetin-Loaded PLGA Nanoparticles as a Pre-Treatment Agent on Indocyanine-Photodynamic Therapy of Breast Cancer Cell |
| title_short | The Role of Crocetin-Loaded PLGA Nanoparticles as a Pre-Treatment Agent on Indocyanine-Photodynamic Therapy of Breast Cancer Cell |
| title_sort | role of crocetin loaded plga nanoparticles as a pre treatment agent on indocyanine photodynamic therapy of breast cancer cell |
| topic | crocetin (crt) poly lactic co glycolic acid (plga) nanoparticles (nps) photodynamic therapy (pdt) indocyanine green (icg) breast cancer |
| url | https://ijmp.mums.ac.ir/article_17845_325ecc903feabcb15df406ddf5acd96e.pdf |
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