Addressing variability in iPSC-derived models of human disease: guidelines to promote reproducibility
Induced pluripotent stem cell (iPSC) technologies have provided in vitro models of inaccessible human cell types, yielding new insights into disease mechanisms especially for neurological disorders. However, without due consideration, the thousands of new human iPSC lines generated in the past decad...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2020-01-01
|
| Series: | Disease Models & Mechanisms |
| Subjects: | |
| Online Access: | http://dmm.biologists.org/content/13/1/dmm042317 |
| _version_ | 1818274490056966144 |
|---|---|
| author | Viola Volpato Caleb Webber |
| author_facet | Viola Volpato Caleb Webber |
| author_sort | Viola Volpato |
| collection | DOAJ |
| description | Induced pluripotent stem cell (iPSC) technologies have provided in vitro models of inaccessible human cell types, yielding new insights into disease mechanisms especially for neurological disorders. However, without due consideration, the thousands of new human iPSC lines generated in the past decade will inevitably affect the reproducibility of iPSC-based experiments. Differences between donor individuals, genetic stability and experimental variability contribute to iPSC model variation by impacting differentiation potency, cellular heterogeneity, morphology, and transcript and protein abundance. Such effects will confound reproducible disease modelling in the absence of appropriate strategies. In this Review, we explore the causes and effects of iPSC heterogeneity, and propose approaches to detect and account for experimental variation between studies, or even exploit it for deeper biological insight. |
| first_indexed | 2024-12-12T22:14:41Z |
| format | Article |
| id | doaj.art-a4465fcd294b428e937a1e64ceedd66b |
| institution | Directory Open Access Journal |
| issn | 1754-8403 1754-8411 |
| language | English |
| last_indexed | 2024-12-12T22:14:41Z |
| publishDate | 2020-01-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Disease Models & Mechanisms |
| spelling | doaj.art-a4465fcd294b428e937a1e64ceedd66b2022-12-22T00:10:08ZengThe Company of BiologistsDisease Models & Mechanisms1754-84031754-84112020-01-0113110.1242/dmm.042317042317Addressing variability in iPSC-derived models of human disease: guidelines to promote reproducibilityViola Volpato0Caleb Webber1 UK Dementia Research Institute at Cardiff University, Division of Psychological Medicine and Clinical Neuroscience, Haydn Ellis Building, Maindy Rd, Cardiff CF24 4HQ, UK UK Dementia Research Institute at Cardiff University, Division of Psychological Medicine and Clinical Neuroscience, Haydn Ellis Building, Maindy Rd, Cardiff CF24 4HQ, UK Induced pluripotent stem cell (iPSC) technologies have provided in vitro models of inaccessible human cell types, yielding new insights into disease mechanisms especially for neurological disorders. However, without due consideration, the thousands of new human iPSC lines generated in the past decade will inevitably affect the reproducibility of iPSC-based experiments. Differences between donor individuals, genetic stability and experimental variability contribute to iPSC model variation by impacting differentiation potency, cellular heterogeneity, morphology, and transcript and protein abundance. Such effects will confound reproducible disease modelling in the absence of appropriate strategies. In this Review, we explore the causes and effects of iPSC heterogeneity, and propose approaches to detect and account for experimental variation between studies, or even exploit it for deeper biological insight.http://dmm.biologists.org/content/13/1/dmm042317bioinformaticscellular heterogeneityreproducibilityipscs |
| spellingShingle | Viola Volpato Caleb Webber Addressing variability in iPSC-derived models of human disease: guidelines to promote reproducibility Disease Models & Mechanisms bioinformatics cellular heterogeneity reproducibility ipscs |
| title | Addressing variability in iPSC-derived models of human disease: guidelines to promote reproducibility |
| title_full | Addressing variability in iPSC-derived models of human disease: guidelines to promote reproducibility |
| title_fullStr | Addressing variability in iPSC-derived models of human disease: guidelines to promote reproducibility |
| title_full_unstemmed | Addressing variability in iPSC-derived models of human disease: guidelines to promote reproducibility |
| title_short | Addressing variability in iPSC-derived models of human disease: guidelines to promote reproducibility |
| title_sort | addressing variability in ipsc derived models of human disease guidelines to promote reproducibility |
| topic | bioinformatics cellular heterogeneity reproducibility ipscs |
| url | http://dmm.biologists.org/content/13/1/dmm042317 |
| work_keys_str_mv | AT violavolpato addressingvariabilityinipscderivedmodelsofhumandiseaseguidelinestopromotereproducibility AT calebwebber addressingvariabilityinipscderivedmodelsofhumandiseaseguidelinestopromotereproducibility |