Site-Dependent Lineage Preference of Adipose Stem Cells
Adult stem cells have unique properties in both proliferation and differentiation preference. In this study, we hypothesized that adipose stem cells have a depot-dependent lineage preference. Four rabbits were used to provide donor-matched adipose stem cells from either subcutaneous adipose tissue (...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-04-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2020.00237/full |
| _version_ | 1811290966480388096 |
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| author | Tingliang Wang Tingliang Wang Ryan C. Hill Monika Dzieciatkowska Lian Zhu Aniello M. Infante Gangqing Hu Gangqing Hu Kirk C. Hansen Ming Pei Ming Pei |
| author_facet | Tingliang Wang Tingliang Wang Ryan C. Hill Monika Dzieciatkowska Lian Zhu Aniello M. Infante Gangqing Hu Gangqing Hu Kirk C. Hansen Ming Pei Ming Pei |
| author_sort | Tingliang Wang |
| collection | DOAJ |
| description | Adult stem cells have unique properties in both proliferation and differentiation preference. In this study, we hypothesized that adipose stem cells have a depot-dependent lineage preference. Four rabbits were used to provide donor-matched adipose stem cells from either subcutaneous adipose tissue (ScAT) or infrapatellar fat pad (IPFP). Proliferation and multi-lineage differentiation were evaluated in adipose stem cells from donor-matched ScAT and IPFP. RNA sequencing (RNA-seq) and proteomics were conducted to uncover potential molecular discrepancy in adipose stem cells and their corresponding matrix microenvironments. We found that stem cells from ScAT exhibited significantly higher proliferation and adipogenic capacity compared to those from donor-matched IPFP while stem cells from IPFP displayed significantly higher chondrogenic potential compared to those from donor-matched ScAT. Our findings are strongly endorsed by supportive data from transcriptome and proteomics analyses, indicating a site-dependent lineage preference of adipose stem cells. |
| first_indexed | 2024-04-13T04:22:20Z |
| format | Article |
| id | doaj.art-a4469487ad824d34a19e48bff4f85d46 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-13T04:22:20Z |
| publishDate | 2020-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-a4469487ad824d34a19e48bff4f85d462022-12-22T03:02:42ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-04-01810.3389/fcell.2020.00237520117Site-Dependent Lineage Preference of Adipose Stem CellsTingliang Wang0Tingliang Wang1Ryan C. Hill2Monika Dzieciatkowska3Lian Zhu4Aniello M. Infante5Gangqing Hu6Gangqing Hu7Kirk C. Hansen8Ming Pei9Ming Pei10Stem Cell and Tissue Engineering Laboratory, Department of Orthopedics, West Virginia University, Morgantown, WV, United StatesDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, CO, United StatesDepartment of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, CO, United StatesDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaBioinformatics Core Facility, West Virginia University, Morgantown, WV, United StatesBioinformatics Core Facility, West Virginia University, Morgantown, WV, United StatesDepartment of Microbiology, Immunology and Cell Biology, School of Medicine, West Virginia University, Morgantown, WV, United StatesDepartment of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, CO, United StatesStem Cell and Tissue Engineering Laboratory, Department of Orthopedics, West Virginia University, Morgantown, WV, United StatesWVU Cancer Institute, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, WV, United StatesAdult stem cells have unique properties in both proliferation and differentiation preference. In this study, we hypothesized that adipose stem cells have a depot-dependent lineage preference. Four rabbits were used to provide donor-matched adipose stem cells from either subcutaneous adipose tissue (ScAT) or infrapatellar fat pad (IPFP). Proliferation and multi-lineage differentiation were evaluated in adipose stem cells from donor-matched ScAT and IPFP. RNA sequencing (RNA-seq) and proteomics were conducted to uncover potential molecular discrepancy in adipose stem cells and their corresponding matrix microenvironments. We found that stem cells from ScAT exhibited significantly higher proliferation and adipogenic capacity compared to those from donor-matched IPFP while stem cells from IPFP displayed significantly higher chondrogenic potential compared to those from donor-matched ScAT. Our findings are strongly endorsed by supportive data from transcriptome and proteomics analyses, indicating a site-dependent lineage preference of adipose stem cells.https://www.frontiersin.org/article/10.3389/fcell.2020.00237/fulladipogenesisadipose stem cellchondrogenesisinfrapatellar fat padosteogenesissubcutaneous adipose tissue |
| spellingShingle | Tingliang Wang Tingliang Wang Ryan C. Hill Monika Dzieciatkowska Lian Zhu Aniello M. Infante Gangqing Hu Gangqing Hu Kirk C. Hansen Ming Pei Ming Pei Site-Dependent Lineage Preference of Adipose Stem Cells Frontiers in Cell and Developmental Biology adipogenesis adipose stem cell chondrogenesis infrapatellar fat pad osteogenesis subcutaneous adipose tissue |
| title | Site-Dependent Lineage Preference of Adipose Stem Cells |
| title_full | Site-Dependent Lineage Preference of Adipose Stem Cells |
| title_fullStr | Site-Dependent Lineage Preference of Adipose Stem Cells |
| title_full_unstemmed | Site-Dependent Lineage Preference of Adipose Stem Cells |
| title_short | Site-Dependent Lineage Preference of Adipose Stem Cells |
| title_sort | site dependent lineage preference of adipose stem cells |
| topic | adipogenesis adipose stem cell chondrogenesis infrapatellar fat pad osteogenesis subcutaneous adipose tissue |
| url | https://www.frontiersin.org/article/10.3389/fcell.2020.00237/full |
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