Programmable all-DNA hydrogels based on rolling circle and multiprimed chain amplification products
Soft, biocompatible, and tunable materials offer biomedical engineers and material scientists programmable matrices for a variety of biomedical applications. In this regard, DNA hydrogels have emerged as highly promising biomaterials that offer programmable self-assembly, superior biocompatibility,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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AIP Publishing LLC
2023-12-01
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| Series: | APL Bioengineering |
| Online Access: | http://dx.doi.org/10.1063/5.0169063 |
| _version_ | 1827391508375928832 |
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| author | Wildan Hanif Indresh Yadav Erol Hasan Dana Alsulaiman |
| author_facet | Wildan Hanif Indresh Yadav Erol Hasan Dana Alsulaiman |
| author_sort | Wildan Hanif |
| collection | DOAJ |
| description | Soft, biocompatible, and tunable materials offer biomedical engineers and material scientists programmable matrices for a variety of biomedical applications. In this regard, DNA hydrogels have emerged as highly promising biomaterials that offer programmable self-assembly, superior biocompatibility, and the presence of specific molecular identifiable structures. Many types of DNA hydrogels have been developed, yet the programmability of the DNA building blocks has not been fully exploited, and further efforts must be directed toward understanding how to finely tune their properties in a predictable manner. Herein, we develop physically crosslinked all-DNA hydrogels with tunable morphology and controllable biodegradation, based on rolling circle amplification and multiprimed chain amplification products. Through molecular engineering of the DNA sequences and their nano-/microscale architectures, the precursors self-assemble in a controlled manner to produce soft hydrogels in an efficient, cost-effective, and highly tunable manner. Notably, we develop a novel DNA microladder architecture that serves as a framework for modulating the hydrogel properties, including over an order of magnitude change in pore size and up to 50% change in biodegradation rate. Overall, we demonstrate how the properties of this DNA-based biomaterial can be tuned by modulating the amounts of rigid double-stranded DNA chains compared to flexible single-stranded DNA chains, as well as through the precursor architecture. Ultimately, this work opens new avenues for the development of programmable and biodegradable soft materials in which DNA functions not only as a store of genetic information but also as a versatile polymeric biomaterial and molecularly engineered macroscale scaffold. |
| first_indexed | 2024-03-08T17:12:05Z |
| format | Article |
| id | doaj.art-a447696a14444e55a50cdc5333a63b67 |
| institution | Directory Open Access Journal |
| issn | 2473-2877 |
| language | English |
| last_indexed | 2024-03-08T17:12:05Z |
| publishDate | 2023-12-01 |
| publisher | AIP Publishing LLC |
| record_format | Article |
| series | APL Bioengineering |
| spelling | doaj.art-a447696a14444e55a50cdc5333a63b672024-01-03T19:58:04ZengAIP Publishing LLCAPL Bioengineering2473-28772023-12-0174046106046106-1110.1063/5.0169063Programmable all-DNA hydrogels based on rolling circle and multiprimed chain amplification productsWildan Hanif0Indresh Yadav1Erol Hasan2Dana Alsulaiman3 Division of Physical Science and Engineering, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA Division of Physical Science and Engineering, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia Division of Physical Science and Engineering, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi ArabiaSoft, biocompatible, and tunable materials offer biomedical engineers and material scientists programmable matrices for a variety of biomedical applications. In this regard, DNA hydrogels have emerged as highly promising biomaterials that offer programmable self-assembly, superior biocompatibility, and the presence of specific molecular identifiable structures. Many types of DNA hydrogels have been developed, yet the programmability of the DNA building blocks has not been fully exploited, and further efforts must be directed toward understanding how to finely tune their properties in a predictable manner. Herein, we develop physically crosslinked all-DNA hydrogels with tunable morphology and controllable biodegradation, based on rolling circle amplification and multiprimed chain amplification products. Through molecular engineering of the DNA sequences and their nano-/microscale architectures, the precursors self-assemble in a controlled manner to produce soft hydrogels in an efficient, cost-effective, and highly tunable manner. Notably, we develop a novel DNA microladder architecture that serves as a framework for modulating the hydrogel properties, including over an order of magnitude change in pore size and up to 50% change in biodegradation rate. Overall, we demonstrate how the properties of this DNA-based biomaterial can be tuned by modulating the amounts of rigid double-stranded DNA chains compared to flexible single-stranded DNA chains, as well as through the precursor architecture. Ultimately, this work opens new avenues for the development of programmable and biodegradable soft materials in which DNA functions not only as a store of genetic information but also as a versatile polymeric biomaterial and molecularly engineered macroscale scaffold.http://dx.doi.org/10.1063/5.0169063 |
| spellingShingle | Wildan Hanif Indresh Yadav Erol Hasan Dana Alsulaiman Programmable all-DNA hydrogels based on rolling circle and multiprimed chain amplification products APL Bioengineering |
| title | Programmable all-DNA hydrogels based on rolling circle and multiprimed chain amplification products |
| title_full | Programmable all-DNA hydrogels based on rolling circle and multiprimed chain amplification products |
| title_fullStr | Programmable all-DNA hydrogels based on rolling circle and multiprimed chain amplification products |
| title_full_unstemmed | Programmable all-DNA hydrogels based on rolling circle and multiprimed chain amplification products |
| title_short | Programmable all-DNA hydrogels based on rolling circle and multiprimed chain amplification products |
| title_sort | programmable all dna hydrogels based on rolling circle and multiprimed chain amplification products |
| url | http://dx.doi.org/10.1063/5.0169063 |
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