flp-32 Ligand/receptor silencing phenocopy faster plant pathogenic nematodes.
Restrictions on nematicide usage underscore the need for novel control strategies for plant pathogenic nematodes such as Globodera pallida (potato cyst nematode) that impose a significant economic burden on plant cultivation activities. The nematode neuropeptide signalling system is an attractive re...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-02-01
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| Series: | PLoS Pathogens |
| Online Access: | http://europepmc.org/articles/PMC3585147?pdf=render |
| _version_ | 1828380694359834624 |
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| author | Louise E Atkinson Michael Stevenson Ciaran J McCoy Nikki J Marks Colin Fleming Mostafa Zamanian Tim A Day Michael J Kimber Aaron G Maule Angela Mousley |
| author_facet | Louise E Atkinson Michael Stevenson Ciaran J McCoy Nikki J Marks Colin Fleming Mostafa Zamanian Tim A Day Michael J Kimber Aaron G Maule Angela Mousley |
| author_sort | Louise E Atkinson |
| collection | DOAJ |
| description | Restrictions on nematicide usage underscore the need for novel control strategies for plant pathogenic nematodes such as Globodera pallida (potato cyst nematode) that impose a significant economic burden on plant cultivation activities. The nematode neuropeptide signalling system is an attractive resource for novel control targets as it plays a critical role in sensory and motor functions. The FMRFamide-like peptides (FLPs) form the largest and most diverse family of neuropeptides in invertebrates, and are structurally conserved across nematode species, highlighting the utility of the FLPergic system as a broad-spectrum control target. flp-32 is expressed widely across nematode species. This study investigates the role of flp-32 in G. pallida and shows that: (i) Gp-flp-32 encodes the peptide AMRNALVRFamide; (ii) Gp-flp-32 is expressed in the brain and ventral nerve cord of G. pallida; (iii) migration rate increases in Gp-flp-32-silenced worms; (iv) the ability of G. pallida to infect potato plant root systems is enhanced in Gp-flp-32-silenced worms; (v) a novel putative Gp-flp-32 receptor (Gp-flp-32R) is expressed in G. pallida; and, (vi) Gp-flp-32R-silenced worms also display an increase in migration rate. This work demonstrates that Gp-flp-32 plays an intrinsic role in the modulation of locomotory behaviour in G. pallida and putatively interacts with at least one novel G-protein coupled receptor (Gp-flp-32R). This is the first functional characterisation of a parasitic nematode FLP-GPCR. |
| first_indexed | 2024-12-10T04:02:46Z |
| format | Article |
| id | doaj.art-a447def2f47b4538b5c3fb7fb93b6f50 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-10T04:02:46Z |
| publishDate | 2013-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-a447def2f47b4538b5c3fb7fb93b6f502022-12-22T02:02:57ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742013-02-0192e100316910.1371/journal.ppat.1003169flp-32 Ligand/receptor silencing phenocopy faster plant pathogenic nematodes.Louise E AtkinsonMichael StevensonCiaran J McCoyNikki J MarksColin FlemingMostafa ZamanianTim A DayMichael J KimberAaron G MauleAngela MousleyRestrictions on nematicide usage underscore the need for novel control strategies for plant pathogenic nematodes such as Globodera pallida (potato cyst nematode) that impose a significant economic burden on plant cultivation activities. The nematode neuropeptide signalling system is an attractive resource for novel control targets as it plays a critical role in sensory and motor functions. The FMRFamide-like peptides (FLPs) form the largest and most diverse family of neuropeptides in invertebrates, and are structurally conserved across nematode species, highlighting the utility of the FLPergic system as a broad-spectrum control target. flp-32 is expressed widely across nematode species. This study investigates the role of flp-32 in G. pallida and shows that: (i) Gp-flp-32 encodes the peptide AMRNALVRFamide; (ii) Gp-flp-32 is expressed in the brain and ventral nerve cord of G. pallida; (iii) migration rate increases in Gp-flp-32-silenced worms; (iv) the ability of G. pallida to infect potato plant root systems is enhanced in Gp-flp-32-silenced worms; (v) a novel putative Gp-flp-32 receptor (Gp-flp-32R) is expressed in G. pallida; and, (vi) Gp-flp-32R-silenced worms also display an increase in migration rate. This work demonstrates that Gp-flp-32 plays an intrinsic role in the modulation of locomotory behaviour in G. pallida and putatively interacts with at least one novel G-protein coupled receptor (Gp-flp-32R). This is the first functional characterisation of a parasitic nematode FLP-GPCR.http://europepmc.org/articles/PMC3585147?pdf=render |
| spellingShingle | Louise E Atkinson Michael Stevenson Ciaran J McCoy Nikki J Marks Colin Fleming Mostafa Zamanian Tim A Day Michael J Kimber Aaron G Maule Angela Mousley flp-32 Ligand/receptor silencing phenocopy faster plant pathogenic nematodes. PLoS Pathogens |
| title | flp-32 Ligand/receptor silencing phenocopy faster plant pathogenic nematodes. |
| title_full | flp-32 Ligand/receptor silencing phenocopy faster plant pathogenic nematodes. |
| title_fullStr | flp-32 Ligand/receptor silencing phenocopy faster plant pathogenic nematodes. |
| title_full_unstemmed | flp-32 Ligand/receptor silencing phenocopy faster plant pathogenic nematodes. |
| title_short | flp-32 Ligand/receptor silencing phenocopy faster plant pathogenic nematodes. |
| title_sort | flp 32 ligand receptor silencing phenocopy faster plant pathogenic nematodes |
| url | http://europepmc.org/articles/PMC3585147?pdf=render |
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