Multiplex Detection of <i>Pleurotus ostreatus</i> Mycoviruses
Mycoviruses are viruses that specifically infect and replicate in fungi. Several mycoviruses have been previously reported in <i>Pleurotus ostreatus</i>, including the oyster mushroom spherical virus (OMSV), oyster mushroom isometric virus (OMIV), <i>Pleurotus ostreatus</i> s...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
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| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/44/11/392 |
| Summary: | Mycoviruses are viruses that specifically infect and replicate in fungi. Several mycoviruses have been previously reported in <i>Pleurotus ostreatus</i>, including the oyster mushroom spherical virus (OMSV), oyster mushroom isometric virus (OMIV), <i>Pleurotus ostreatus</i> spherical virus (POSV), and <i>Pleurotus ostreatus</i> virus 1 (PoV1). This study was designed to develop a multiplex RT-PCR for simultaneous detection and differentiation of the four <i>P. ostreatus</i> mycoviruses. Four pairs of primers were designed from conserved regions based on the reported sequences and the multiplex RT-PCR products were 672 bp for OMSV, 540 bp for OMIV, 310 bp for POSV, and 200 bp for PoV1. The optimal annealing temperature of the multiplex RT-PCR was 62 °C and the detection limits of the plasmids were 100 fg for OMSV and OMIV and 1 pg for POSV and PoV1. This technique was successfully applied for the detection of OMSV, OMIV, and POSV from different <i>P. ostreatus</i> strains and the plasmid containing the PoV1 sequence. This methodology can serve as a powerful diagnostic tool for the survey of the incidence and epidemiology of the four <i>P. ostreatus</i> mycoviruses, further contributing to the prevention and treatment of mycoviral diseases in <i>P. ostreatus</i>. |
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| ISSN: | 1467-3037 1467-3045 |