Protein Chemical Labeling Using Biomimetic Radical Chemistry
Chemical labeling of proteins with synthetic low-molecular-weight probes is an important technique in chemical biology. To achieve this, it is necessary to use chemical reactions that proceed rapidly under physiological conditions (i.e., aqueous solvent, pH, low concentration, and low temperature) s...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/24/21/3980 |
| _version_ | 1819113307578040320 |
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| author | Shinichi Sato Hiroyuki Nakamura |
| author_facet | Shinichi Sato Hiroyuki Nakamura |
| author_sort | Shinichi Sato |
| collection | DOAJ |
| description | Chemical labeling of proteins with synthetic low-molecular-weight probes is an important technique in chemical biology. To achieve this, it is necessary to use chemical reactions that proceed rapidly under physiological conditions (i.e., aqueous solvent, pH, low concentration, and low temperature) so that protein denaturation does not occur. The radical reaction satisfies such demands of protein labeling, and protein labeling using the biomimetic radical reaction has recently attracted attention. The biomimetic radical reaction enables selective labeling of the C-terminus, tyrosine, and tryptophan, which is difficult to achieve with conventional electrophilic protein labeling. In addition, as the radical reaction proceeds selectively in close proximity to the catalyst, it can be applied to the analysis of protein−protein interactions. In this review, recent trends in protein labeling using biomimetic radical reactions are discussed. |
| first_indexed | 2024-12-22T04:27:20Z |
| format | Article |
| id | doaj.art-a44bfe348e434e428b0af938797c9975 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T04:27:20Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a44bfe348e434e428b0af938797c99752022-12-21T18:39:07ZengMDPI AGMolecules1420-30492019-11-012421398010.3390/molecules24213980molecules24213980Protein Chemical Labeling Using Biomimetic Radical ChemistryShinichi Sato0Hiroyuki Nakamura1Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, JapanLaboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, JapanChemical labeling of proteins with synthetic low-molecular-weight probes is an important technique in chemical biology. To achieve this, it is necessary to use chemical reactions that proceed rapidly under physiological conditions (i.e., aqueous solvent, pH, low concentration, and low temperature) so that protein denaturation does not occur. The radical reaction satisfies such demands of protein labeling, and protein labeling using the biomimetic radical reaction has recently attracted attention. The biomimetic radical reaction enables selective labeling of the C-terminus, tyrosine, and tryptophan, which is difficult to achieve with conventional electrophilic protein labeling. In addition, as the radical reaction proceeds selectively in close proximity to the catalyst, it can be applied to the analysis of protein−protein interactions. In this review, recent trends in protein labeling using biomimetic radical reactions are discussed.https://www.mdpi.com/1420-3049/24/21/3980biomimetic radical reactionbioinspired chemical catalysisprotein labeling |
| spellingShingle | Shinichi Sato Hiroyuki Nakamura Protein Chemical Labeling Using Biomimetic Radical Chemistry Molecules biomimetic radical reaction bioinspired chemical catalysis protein labeling |
| title | Protein Chemical Labeling Using Biomimetic Radical Chemistry |
| title_full | Protein Chemical Labeling Using Biomimetic Radical Chemistry |
| title_fullStr | Protein Chemical Labeling Using Biomimetic Radical Chemistry |
| title_full_unstemmed | Protein Chemical Labeling Using Biomimetic Radical Chemistry |
| title_short | Protein Chemical Labeling Using Biomimetic Radical Chemistry |
| title_sort | protein chemical labeling using biomimetic radical chemistry |
| topic | biomimetic radical reaction bioinspired chemical catalysis protein labeling |
| url | https://www.mdpi.com/1420-3049/24/21/3980 |
| work_keys_str_mv | AT shinichisato proteinchemicallabelingusingbiomimeticradicalchemistry AT hiroyukinakamura proteinchemicallabelingusingbiomimeticradicalchemistry |