Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET
Abstract Synthetic vascular smooth muscle cells (VSMCs) play important roles in atherosclerosis, in-stent restenosis, and transplant vasculopathy. We investigated the synthetic activity of VSMCs in the atherosclerotic carotid artery using 18F-fluorodeoxyglucose (FDG) positron emission tomography (PE...
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Nature Portfolio
2017-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-07073-3 |
| _version_ | 1818839728593567744 |
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| author | Kisoo Pahk Chanmin Joung Se-Mi Jung Hwa Young Song Ji Yong Park Jung Woo Byun Yun-Sang Lee Jin Chul Paeng Chunsook Kim Sungeun Kim Won-Ki Kim |
| author_facet | Kisoo Pahk Chanmin Joung Se-Mi Jung Hwa Young Song Ji Yong Park Jung Woo Byun Yun-Sang Lee Jin Chul Paeng Chunsook Kim Sungeun Kim Won-Ki Kim |
| author_sort | Kisoo Pahk |
| collection | DOAJ |
| description | Abstract Synthetic vascular smooth muscle cells (VSMCs) play important roles in atherosclerosis, in-stent restenosis, and transplant vasculopathy. We investigated the synthetic activity of VSMCs in the atherosclerotic carotid artery using 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET). Atherosclerosis was induced in rats by partial ligation of the right carotid artery coupled with an atherogenic diet and vitamin D injections (2 consecutive days, 600,000 IU/day). One month later, rats were imaged by F-18 FDG PET. The atherosclerotic right carotid arteries showed prominent luminal narrowing with neointimal hyperplasia. The regions with neointimal hyperplasia were composed of α-smooth muscle actin-positive cells with decreased expression of smooth muscle myosin heavy chain. Surrogate markers of synthetic VSMCs such as collagen type III, cyclophilin A, and matrix metallopeptidase-9 were increased in neointima region. However, neither macrophages nor neutrophils were observed in regions with neointimal hyperplasia. F-18 FDG PET imaging and autoradiography showed elevated FDG uptake into the atherosclerotic carotid artery. The inner vessel layer showed higher tracer uptake than the outer layer. Consistently, the expression of glucose transporter 1 was highly increased in neointima. The present results indicate that F-18 FDG PET may be a useful tool for evaluating synthetic activities of VSMCs in vascular remodeling disorders. |
| first_indexed | 2024-12-19T03:58:54Z |
| format | Article |
| id | doaj.art-a44ff81d29b34e39887f2315406f4991 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T03:58:54Z |
| publishDate | 2017-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a44ff81d29b34e39887f2315406f49912022-12-21T20:36:44ZengNature PortfolioScientific Reports2045-23222017-08-01711810.1038/s41598-017-07073-3Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PETKisoo Pahk0Chanmin Joung1Se-Mi Jung2Hwa Young Song3Ji Yong Park4Jung Woo Byun5Yun-Sang Lee6Jin Chul Paeng7Chunsook Kim8Sungeun Kim9Won-Ki Kim10Department of Neuroscience, Korea University College of MedicineDepartment of Neuroscience, Korea University College of MedicineDepartment of Neuroscience, Korea University College of MedicineDepartment of Neuroscience, Korea University College of MedicineDepartment of Nuclear Medicine, Seoul National University College of MedicineDepartment of Nuclear Medicine, Seoul National University College of MedicineDepartment of Nuclear Medicine, Seoul National University College of MedicineDepartment of Nuclear Medicine, Seoul National University HospitalDepartment of Nursing, Kyungdong UniversityDepartment of Nuclear Medicine, Korea University Anam HospitalDepartment of Neuroscience, Korea University College of MedicineAbstract Synthetic vascular smooth muscle cells (VSMCs) play important roles in atherosclerosis, in-stent restenosis, and transplant vasculopathy. We investigated the synthetic activity of VSMCs in the atherosclerotic carotid artery using 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET). Atherosclerosis was induced in rats by partial ligation of the right carotid artery coupled with an atherogenic diet and vitamin D injections (2 consecutive days, 600,000 IU/day). One month later, rats were imaged by F-18 FDG PET. The atherosclerotic right carotid arteries showed prominent luminal narrowing with neointimal hyperplasia. The regions with neointimal hyperplasia were composed of α-smooth muscle actin-positive cells with decreased expression of smooth muscle myosin heavy chain. Surrogate markers of synthetic VSMCs such as collagen type III, cyclophilin A, and matrix metallopeptidase-9 were increased in neointima region. However, neither macrophages nor neutrophils were observed in regions with neointimal hyperplasia. F-18 FDG PET imaging and autoradiography showed elevated FDG uptake into the atherosclerotic carotid artery. The inner vessel layer showed higher tracer uptake than the outer layer. Consistently, the expression of glucose transporter 1 was highly increased in neointima. The present results indicate that F-18 FDG PET may be a useful tool for evaluating synthetic activities of VSMCs in vascular remodeling disorders.https://doi.org/10.1038/s41598-017-07073-3 |
| spellingShingle | Kisoo Pahk Chanmin Joung Se-Mi Jung Hwa Young Song Ji Yong Park Jung Woo Byun Yun-Sang Lee Jin Chul Paeng Chunsook Kim Sungeun Kim Won-Ki Kim Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET Scientific Reports |
| title | Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET |
| title_full | Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET |
| title_fullStr | Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET |
| title_full_unstemmed | Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET |
| title_short | Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET |
| title_sort | visualization of synthetic vascular smooth muscle cells in atherosclerotic carotid rat arteries by f 18 fdg pet |
| url | https://doi.org/10.1038/s41598-017-07073-3 |
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