Susceptibility of Staphylococcus aureus Clinical Isolates to Propolis Extract Alone or in Combination with Antimicrobial Drugs
The objective of this study was to assess in vitro the antimicrobial activity of ethanolic extract of Polish propolis (EEPP) against methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates. The combined effect of EEPP and 10 selecte...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-08-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/18/8/9623 |
| _version_ | 1819206055203176448 |
|---|---|
| author | Mieczysław Sajewicz Agata Kabała-Dzik Joanna Smoleń-Dzirba Jerzy Stojko Robert Kubina Małgorzata Kępa Danuta Idzik Robert D. Wojtyczka Arkadiusz Dziedzic Tomasz J. Wąsik |
| author_facet | Mieczysław Sajewicz Agata Kabała-Dzik Joanna Smoleń-Dzirba Jerzy Stojko Robert Kubina Małgorzata Kępa Danuta Idzik Robert D. Wojtyczka Arkadiusz Dziedzic Tomasz J. Wąsik |
| author_sort | Mieczysław Sajewicz |
| collection | DOAJ |
| description | The objective of this study was to assess in vitro the antimicrobial activity of ethanolic extract of Polish propolis (EEPP) against methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates. The combined effect of EEPP and 10 selected antistaphylococcal drugs on S. aureus clinical cultures was also investigated. EEPP composition was analyzed by a High Performance Liquid Chromatography (HPLC) method. The flavonoid compounds identified in Polish Propolis included flavones, flavonones, flavonolols, flavonols and phenolic acids. EEPP displayed varying effectiveness against twelve S. aureus strains, with minimal inhibitory concentration (MIC) within the range from 0.39 to 0.78 mg/mL, determined by broth microdilution method. The average MIC was 0.54 ± 0.22 mg/mL, while calculated MIC50 and MIC90 were 0.39 mg/mL and 0.78 mg/mL, respectively. The minimum bactericidal concentration (MBC) of the EEPP ranged from 0.78 to 3.13 mg/mL. The in vitro combined effect of EEPP and 10 antibacterial drugs was investigated using disk diffusion method-based assay. Addition of EEPP to cefoxitin (FOX), clindamycin (DA), tetracycline (TE), tobramycin (TOB), linezolid (LIN), trimethoprim+sulfamethoxazole (SXT), penicillin (P), erythromycin (E) regimen, yielded stronger, cumulative antimicrobial effect, against all tested S. aureus strains than EEPP and chemotherapeutics alone. In the case of ciprofloxacin (CIP) and chloramphenicol (C) no synergism with EEPP was observed. |
| first_indexed | 2024-12-23T05:01:31Z |
| format | Article |
| id | doaj.art-a450035a25784a8f80c8fdbf837acdb8 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-23T05:01:31Z |
| publishDate | 2013-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a450035a25784a8f80c8fdbf837acdb82022-12-21T17:59:13ZengMDPI AGMolecules1420-30492013-08-011889623964010.3390/molecules18089623Susceptibility of Staphylococcus aureus Clinical Isolates to Propolis Extract Alone or in Combination with Antimicrobial DrugsMieczysław SajewiczAgata Kabała-DzikJoanna Smoleń-DzirbaJerzy StojkoRobert KubinaMałgorzata KępaDanuta IdzikRobert D. WojtyczkaArkadiusz DziedzicTomasz J. WąsikThe objective of this study was to assess in vitro the antimicrobial activity of ethanolic extract of Polish propolis (EEPP) against methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates. The combined effect of EEPP and 10 selected antistaphylococcal drugs on S. aureus clinical cultures was also investigated. EEPP composition was analyzed by a High Performance Liquid Chromatography (HPLC) method. The flavonoid compounds identified in Polish Propolis included flavones, flavonones, flavonolols, flavonols and phenolic acids. EEPP displayed varying effectiveness against twelve S. aureus strains, with minimal inhibitory concentration (MIC) within the range from 0.39 to 0.78 mg/mL, determined by broth microdilution method. The average MIC was 0.54 ± 0.22 mg/mL, while calculated MIC50 and MIC90 were 0.39 mg/mL and 0.78 mg/mL, respectively. The minimum bactericidal concentration (MBC) of the EEPP ranged from 0.78 to 3.13 mg/mL. The in vitro combined effect of EEPP and 10 antibacterial drugs was investigated using disk diffusion method-based assay. Addition of EEPP to cefoxitin (FOX), clindamycin (DA), tetracycline (TE), tobramycin (TOB), linezolid (LIN), trimethoprim+sulfamethoxazole (SXT), penicillin (P), erythromycin (E) regimen, yielded stronger, cumulative antimicrobial effect, against all tested S. aureus strains than EEPP and chemotherapeutics alone. In the case of ciprofloxacin (CIP) and chloramphenicol (C) no synergism with EEPP was observed.http://www.mdpi.com/1420-3049/18/8/9623methicillin-resistant S. aureus (MRSA)methicillin-sensitive S. aureus (MSSA)ethanolic extract of Polish propolis (EEPP) |
| spellingShingle | Mieczysław Sajewicz Agata Kabała-Dzik Joanna Smoleń-Dzirba Jerzy Stojko Robert Kubina Małgorzata Kępa Danuta Idzik Robert D. Wojtyczka Arkadiusz Dziedzic Tomasz J. Wąsik Susceptibility of Staphylococcus aureus Clinical Isolates to Propolis Extract Alone or in Combination with Antimicrobial Drugs Molecules methicillin-resistant S. aureus (MRSA) methicillin-sensitive S. aureus (MSSA) ethanolic extract of Polish propolis (EEPP) |
| title | Susceptibility of Staphylococcus aureus Clinical Isolates to Propolis Extract Alone or in Combination with Antimicrobial Drugs |
| title_full | Susceptibility of Staphylococcus aureus Clinical Isolates to Propolis Extract Alone or in Combination with Antimicrobial Drugs |
| title_fullStr | Susceptibility of Staphylococcus aureus Clinical Isolates to Propolis Extract Alone or in Combination with Antimicrobial Drugs |
| title_full_unstemmed | Susceptibility of Staphylococcus aureus Clinical Isolates to Propolis Extract Alone or in Combination with Antimicrobial Drugs |
| title_short | Susceptibility of Staphylococcus aureus Clinical Isolates to Propolis Extract Alone or in Combination with Antimicrobial Drugs |
| title_sort | susceptibility of staphylococcus aureus clinical isolates to propolis extract alone or in combination with antimicrobial drugs |
| topic | methicillin-resistant S. aureus (MRSA) methicillin-sensitive S. aureus (MSSA) ethanolic extract of Polish propolis (EEPP) |
| url | http://www.mdpi.com/1420-3049/18/8/9623 |
| work_keys_str_mv | AT mieczysławsajewicz susceptibilityofstaphylococcusaureusclinicalisolatestopropolisextractaloneorincombinationwithantimicrobialdrugs AT agatakabaładzik susceptibilityofstaphylococcusaureusclinicalisolatestopropolisextractaloneorincombinationwithantimicrobialdrugs AT joannasmolendzirba susceptibilityofstaphylococcusaureusclinicalisolatestopropolisextractaloneorincombinationwithantimicrobialdrugs AT jerzystojko susceptibilityofstaphylococcusaureusclinicalisolatestopropolisextractaloneorincombinationwithantimicrobialdrugs AT robertkubina susceptibilityofstaphylococcusaureusclinicalisolatestopropolisextractaloneorincombinationwithantimicrobialdrugs AT małgorzatakepa susceptibilityofstaphylococcusaureusclinicalisolatestopropolisextractaloneorincombinationwithantimicrobialdrugs AT danutaidzik susceptibilityofstaphylococcusaureusclinicalisolatestopropolisextractaloneorincombinationwithantimicrobialdrugs AT robertdwojtyczka susceptibilityofstaphylococcusaureusclinicalisolatestopropolisextractaloneorincombinationwithantimicrobialdrugs AT arkadiuszdziedzic susceptibilityofstaphylococcusaureusclinicalisolatestopropolisextractaloneorincombinationwithantimicrobialdrugs AT tomaszjwasik susceptibilityofstaphylococcusaureusclinicalisolatestopropolisextractaloneorincombinationwithantimicrobialdrugs |