Biorefinery-Based Approach to Exploit Mixed Cultures of <i>Lipomyces starkeyi</i> and <i>Chloroidium saccharophilum</i> for Single Cell Oil Production
The mutualistic interactions between the oleaginous yeast <i>Lipomyces starkeyi</i> and the green microalga <i>Chloroidium saccharophilum</i> in mixed cultures were investigated to exploit possible synergistic effects. In fact, microalga could act as an oxygen generator for t...
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MDPI AG
2021-03-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/14/5/1340 |
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| author | Gaetano Zuccaro Angelo del Mondo Gabriele Pinto Antonino Pollio Antonino De Natale |
| author_facet | Gaetano Zuccaro Angelo del Mondo Gabriele Pinto Antonino Pollio Antonino De Natale |
| author_sort | Gaetano Zuccaro |
| collection | DOAJ |
| description | The mutualistic interactions between the oleaginous yeast <i>Lipomyces starkeyi</i> and the green microalga <i>Chloroidium saccharophilum</i> in mixed cultures were investigated to exploit possible synergistic effects. In fact, microalga could act as an oxygen generator for the yeast, while the yeast could provide carbon dioxide to microalga. The behavior of the two microorganisms alone and in mixed culture was studied in two synthetic media (YEG and BBM + G) before moving on to a real model represented by the hydrolysate of <i>Arundo donax</i>, used as low-cost feedstock, and previously subjected to steam explosion and enzymatic hydrolysis. The overall lipid content and lipid productivity obtained in the mixed culture of YEG, BBM + G and for the hydrolysate of <i>Arundo donax</i> were equal to 0.064, 0.064 and 0.081 g<sub>lipid</sub>·g<sub>biomass</sub><sup>−1</sup> and 30.14, 35.56 and 37.22 mg<sub>lipid</sub>·L<sup>−1</sup>·day<sup>−1</sup>, respectively. The mixed cultures, in all cases, proved to be the most performing compared to the individual ones. In addition, this study provided new input for the integration of Single Cell Oil (SCO) production with agro-industrial feedstock, and the fatty acid distribution mainly consisting of stearic (C18:0) and oleic acid (C18:1) allows promising applications in biofuels, cosmetics, food additives and other products of industrial interest. |
| first_indexed | 2024-03-09T06:04:35Z |
| format | Article |
| id | doaj.art-a45b76572e914326bf15b18f483c19a1 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-09T06:04:35Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-a45b76572e914326bf15b18f483c19a12023-12-03T12:06:06ZengMDPI AGEnergies1996-10732021-03-01145134010.3390/en14051340Biorefinery-Based Approach to Exploit Mixed Cultures of <i>Lipomyces starkeyi</i> and <i>Chloroidium saccharophilum</i> for Single Cell Oil ProductionGaetano Zuccaro0Angelo del Mondo1Gabriele Pinto2Antonino Pollio3Antonino De Natale4Department of Chemical, Materials and Production Engineering, University of Naples Federico II, 80125 Napoli, ItalyStazione Zoologica Anton Dohrn, Istituto Nazionale di Biologia, Ecologia e Biotecnologie Marine, Villa Comunale, 80121 Napoli, ItalyDipartimento di Biologia, Universita’ degli Studi di Napoli Federico II, Via Cinthia 26, 80126 Napoli, ItalyDipartimento di Biologia, Universita’ degli Studi di Napoli Federico II, Via Cinthia 26, 80126 Napoli, ItalyDipartimento di Biologia, Universita’ degli Studi di Napoli Federico II, Via Cinthia 26, 80126 Napoli, ItalyThe mutualistic interactions between the oleaginous yeast <i>Lipomyces starkeyi</i> and the green microalga <i>Chloroidium saccharophilum</i> in mixed cultures were investigated to exploit possible synergistic effects. In fact, microalga could act as an oxygen generator for the yeast, while the yeast could provide carbon dioxide to microalga. The behavior of the two microorganisms alone and in mixed culture was studied in two synthetic media (YEG and BBM + G) before moving on to a real model represented by the hydrolysate of <i>Arundo donax</i>, used as low-cost feedstock, and previously subjected to steam explosion and enzymatic hydrolysis. The overall lipid content and lipid productivity obtained in the mixed culture of YEG, BBM + G and for the hydrolysate of <i>Arundo donax</i> were equal to 0.064, 0.064 and 0.081 g<sub>lipid</sub>·g<sub>biomass</sub><sup>−1</sup> and 30.14, 35.56 and 37.22 mg<sub>lipid</sub>·L<sup>−1</sup>·day<sup>−1</sup>, respectively. The mixed cultures, in all cases, proved to be the most performing compared to the individual ones. In addition, this study provided new input for the integration of Single Cell Oil (SCO) production with agro-industrial feedstock, and the fatty acid distribution mainly consisting of stearic (C18:0) and oleic acid (C18:1) allows promising applications in biofuels, cosmetics, food additives and other products of industrial interest.https://www.mdpi.com/1996-1073/14/5/1340mixed culture<i>Lipomyces starkeyi</i><i>Chloroidium saccharophilum</i>Single Cell Oils (SCOs)<i>Arundo donax</i>biorefinery |
| spellingShingle | Gaetano Zuccaro Angelo del Mondo Gabriele Pinto Antonino Pollio Antonino De Natale Biorefinery-Based Approach to Exploit Mixed Cultures of <i>Lipomyces starkeyi</i> and <i>Chloroidium saccharophilum</i> for Single Cell Oil Production Energies mixed culture <i>Lipomyces starkeyi</i> <i>Chloroidium saccharophilum</i> Single Cell Oils (SCOs) <i>Arundo donax</i> biorefinery |
| title | Biorefinery-Based Approach to Exploit Mixed Cultures of <i>Lipomyces starkeyi</i> and <i>Chloroidium saccharophilum</i> for Single Cell Oil Production |
| title_full | Biorefinery-Based Approach to Exploit Mixed Cultures of <i>Lipomyces starkeyi</i> and <i>Chloroidium saccharophilum</i> for Single Cell Oil Production |
| title_fullStr | Biorefinery-Based Approach to Exploit Mixed Cultures of <i>Lipomyces starkeyi</i> and <i>Chloroidium saccharophilum</i> for Single Cell Oil Production |
| title_full_unstemmed | Biorefinery-Based Approach to Exploit Mixed Cultures of <i>Lipomyces starkeyi</i> and <i>Chloroidium saccharophilum</i> for Single Cell Oil Production |
| title_short | Biorefinery-Based Approach to Exploit Mixed Cultures of <i>Lipomyces starkeyi</i> and <i>Chloroidium saccharophilum</i> for Single Cell Oil Production |
| title_sort | biorefinery based approach to exploit mixed cultures of i lipomyces starkeyi i and i chloroidium saccharophilum i for single cell oil production |
| topic | mixed culture <i>Lipomyces starkeyi</i> <i>Chloroidium saccharophilum</i> Single Cell Oils (SCOs) <i>Arundo donax</i> biorefinery |
| url | https://www.mdpi.com/1996-1073/14/5/1340 |
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