Insights into the Role of <i>Streptomyces hydrogenans</i> as the Plant Growth Promoter, Photosynthetic Pigment Enhancer and Biocontrol Agent against <i>Meloidogyne incognita</i> in <i>Solanum lycopersicum</i> Seedlings
Root-knot nematodes (RKN), <i>Meloidogyne</i> sp. hinders functioning of crops and causes global losses in terms of productivity and yield. <i>Meloidogyne</i> sp. are microscopic, obligatory endoparasites with ubiquitous distribution in different parts of the world. Taking in...
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MDPI AG
2020-08-01
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| author | Nandni Sharma Kanika Khanna Rajesh Kumari Manhas Renu Bhardwaj Puja Ohri Jawaher Alkahtani Mona S. Alwahibi Parvaiz Ahmad |
| author_facet | Nandni Sharma Kanika Khanna Rajesh Kumari Manhas Renu Bhardwaj Puja Ohri Jawaher Alkahtani Mona S. Alwahibi Parvaiz Ahmad |
| author_sort | Nandni Sharma |
| collection | DOAJ |
| description | Root-knot nematodes (RKN), <i>Meloidogyne</i> sp. hinders functioning of crops and causes global losses in terms of productivity and yield. <i>Meloidogyne</i> sp. are microscopic, obligatory endoparasites with ubiquitous distribution in different parts of the world. Taking into consideration these aspects, the present study was conducted to explore nematicidal activity of the <i>Streptomyces hydrogenans</i> strain DH-16 against <i>M. incognita</i> to regulate its pathogenicity in plants. In-vitro experimentation revealed that pretreated seeds with solvent and culture supernatant lowered root galls in infested plants and promoted growth of <i>Solanum lycopersicum</i> seedlings, revealed through the morphological analysis. Additionally, antioxidative defense responses were induced with microbes. However, oxidative stress markers were considerably reduced after microbial inoculations. Apart from this, secondary metabolites were assessed and modulated in RKN infested plants on microbial supplementations. Confocal studies evaluated glutathione accumulation within root apices and its enhancement was directly proportional to defense responses. Therefore, the current study concluded the role of <i>S. hydrogenans</i> in stimulating antioxidant potential against RKN along with growth promoting aids. Thus, the outcome of the current study endorses that metabolites produced by <i>S. hydrogenans</i> can be used as safe biocontrol agents against <i>M. incognita</i> and also as plant growth promoting agents. |
| first_indexed | 2024-03-10T16:46:04Z |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-03-10T16:46:04Z |
| publishDate | 2020-08-01 |
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| series | Plants |
| spelling | doaj.art-a45beb5d0e84484988c25a13ad9970ca2023-11-20T11:38:34ZengMDPI AGPlants2223-77472020-08-0199110910.3390/plants9091109Insights into the Role of <i>Streptomyces hydrogenans</i> as the Plant Growth Promoter, Photosynthetic Pigment Enhancer and Biocontrol Agent against <i>Meloidogyne incognita</i> in <i>Solanum lycopersicum</i> SeedlingsNandni Sharma0Kanika Khanna1Rajesh Kumari Manhas2Renu Bhardwaj3Puja Ohri4Jawaher Alkahtani5Mona S. Alwahibi6Parvaiz Ahmad7Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab 143005, IndiaDepartment of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005, IndiaDepartment of Microbiology, Guru Nanak Dev University, Amritsar, Punjab 143005, IndiaDepartment of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005, IndiaDepartment of Zoology, Guru Nanak Dev University, Amritsar, Punjab 143005, IndiaBotany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaBotany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaBotany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaRoot-knot nematodes (RKN), <i>Meloidogyne</i> sp. hinders functioning of crops and causes global losses in terms of productivity and yield. <i>Meloidogyne</i> sp. are microscopic, obligatory endoparasites with ubiquitous distribution in different parts of the world. Taking into consideration these aspects, the present study was conducted to explore nematicidal activity of the <i>Streptomyces hydrogenans</i> strain DH-16 against <i>M. incognita</i> to regulate its pathogenicity in plants. In-vitro experimentation revealed that pretreated seeds with solvent and culture supernatant lowered root galls in infested plants and promoted growth of <i>Solanum lycopersicum</i> seedlings, revealed through the morphological analysis. Additionally, antioxidative defense responses were induced with microbes. However, oxidative stress markers were considerably reduced after microbial inoculations. Apart from this, secondary metabolites were assessed and modulated in RKN infested plants on microbial supplementations. Confocal studies evaluated glutathione accumulation within root apices and its enhancement was directly proportional to defense responses. Therefore, the current study concluded the role of <i>S. hydrogenans</i> in stimulating antioxidant potential against RKN along with growth promoting aids. Thus, the outcome of the current study endorses that metabolites produced by <i>S. hydrogenans</i> can be used as safe biocontrol agents against <i>M. incognita</i> and also as plant growth promoting agents.https://www.mdpi.com/2223-7747/9/9/1109<i>Meloidogyne incognita</i><i>Streptomyces hydrogenans</i>photosynthesisoxidative damageantioxidantsconfocal microscopy |
| spellingShingle | Nandni Sharma Kanika Khanna Rajesh Kumari Manhas Renu Bhardwaj Puja Ohri Jawaher Alkahtani Mona S. Alwahibi Parvaiz Ahmad Insights into the Role of <i>Streptomyces hydrogenans</i> as the Plant Growth Promoter, Photosynthetic Pigment Enhancer and Biocontrol Agent against <i>Meloidogyne incognita</i> in <i>Solanum lycopersicum</i> Seedlings Plants <i>Meloidogyne incognita</i> <i>Streptomyces hydrogenans</i> photosynthesis oxidative damage antioxidants confocal microscopy |
| title | Insights into the Role of <i>Streptomyces hydrogenans</i> as the Plant Growth Promoter, Photosynthetic Pigment Enhancer and Biocontrol Agent against <i>Meloidogyne incognita</i> in <i>Solanum lycopersicum</i> Seedlings |
| title_full | Insights into the Role of <i>Streptomyces hydrogenans</i> as the Plant Growth Promoter, Photosynthetic Pigment Enhancer and Biocontrol Agent against <i>Meloidogyne incognita</i> in <i>Solanum lycopersicum</i> Seedlings |
| title_fullStr | Insights into the Role of <i>Streptomyces hydrogenans</i> as the Plant Growth Promoter, Photosynthetic Pigment Enhancer and Biocontrol Agent against <i>Meloidogyne incognita</i> in <i>Solanum lycopersicum</i> Seedlings |
| title_full_unstemmed | Insights into the Role of <i>Streptomyces hydrogenans</i> as the Plant Growth Promoter, Photosynthetic Pigment Enhancer and Biocontrol Agent against <i>Meloidogyne incognita</i> in <i>Solanum lycopersicum</i> Seedlings |
| title_short | Insights into the Role of <i>Streptomyces hydrogenans</i> as the Plant Growth Promoter, Photosynthetic Pigment Enhancer and Biocontrol Agent against <i>Meloidogyne incognita</i> in <i>Solanum lycopersicum</i> Seedlings |
| title_sort | insights into the role of i streptomyces hydrogenans i as the plant growth promoter photosynthetic pigment enhancer and biocontrol agent against i meloidogyne incognita i in i solanum lycopersicum i seedlings |
| topic | <i>Meloidogyne incognita</i> <i>Streptomyces hydrogenans</i> photosynthesis oxidative damage antioxidants confocal microscopy |
| url | https://www.mdpi.com/2223-7747/9/9/1109 |
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