Exploring the Antifungal Activity and Action of <i>Saussurea costus</i> Root Extracts against <i>Candida albicans</i> and Non-<i>albicans</i> Species
The isolation and assessment of the active constituents in polar and non-polar crude extracts of <i>Saussurea costus</i> roots as antifungal agents, against <i>Candida albicans</i> and non-<i>C</i>. <i>albicans</i> (NAC) species, was the aim of this cu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/3/327 |
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| author | Melad F. Soliman Youssria M. Shetaia Ahmed A. Tayel Alaa M. Munshi Fuad A. Alatawi Mohammed A. Alsieni Mahmoud A. Al-Saman |
| author_facet | Melad F. Soliman Youssria M. Shetaia Ahmed A. Tayel Alaa M. Munshi Fuad A. Alatawi Mohammed A. Alsieni Mahmoud A. Al-Saman |
| author_sort | Melad F. Soliman |
| collection | DOAJ |
| description | The isolation and assessment of the active constituents in polar and non-polar crude extracts of <i>Saussurea costus</i> roots as antifungal agents, against <i>Candida albicans</i> and non-<i>C</i>. <i>albicans</i> (NAC) species, was the aim of this current investigation. The SEM “Scanning electron microscopy” imaging provided potential action modes of <i>n</i>-hexane extract (<i>nh</i>hE) toward <i>Candida</i> spp., whereas the TLC-DB “Thin layer chromatography-direct bioautography” was employed for detecting the anticandidal compounds. <i>nh</i>hE had the greatest biocidal activity against all strains and clinical isolates of <i>Candida</i> spp. with maximum zones of inhibition. SEM revealed the occurrence of irregular, dense inclusions of <i>C. albicans</i> cell walls after treatment with <i>nh</i>hE for 12 h. Complete morphological distortions with lysed membranes and deterioration signs appeared in most treated cells of <i>C. parapsilosis</i>. The most effectual compound with anticandidal activity was isolated using TLC-BD and identified as sesquiterpene by GC/MS analysis. The infra-red analysis revealed the presence of lactone ring stretching vibrations at 1766.72 cm<sup>−1</sup>. The anticandidal activity of <i>nh</i>hE of <i>S. costus</i> roots was confirmed from the results, and the treated cotton fabrics with <i>nh</i>hE of <i>S. costus</i> possessed observable activity against <i>C. albicans</i>. Data could recommend the practical usage of <i>S. costus</i> extracts, particularly <i>nh</i>hE, as influential natural bioactive sources for combating pathogenic <i>Candida</i> spp. |
| first_indexed | 2024-03-09T20:12:49Z |
| format | Article |
| id | doaj.art-a462efba7f3f47d384f1b7960a9e415e |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T20:12:49Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-a462efba7f3f47d384f1b7960a9e415e2023-11-24T00:10:43ZengMDPI AGAntibiotics2079-63822022-03-0111332710.3390/antibiotics11030327Exploring the Antifungal Activity and Action of <i>Saussurea costus</i> Root Extracts against <i>Candida albicans</i> and Non-<i>albicans</i> SpeciesMelad F. Soliman0Youssria M. Shetaia1Ahmed A. Tayel2Alaa M. Munshi3Fuad A. Alatawi4Mohammed A. Alsieni5Mahmoud A. Al-Saman6Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City (USsC), Sadat City 22857, EgyptDepartment of Microbiology, Faculty of Science, Ain Shams University, Cairo 11566, EgyptFaculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafrelsheikh 33516, EgyptDepartment of Chemistry, Faculty of Applied Science, Umm Al-Qura University, Mecca 24243, Saudi ArabiaDepartment of Biology, Faculty of Science, University of Tabuk, Tabuk 47512, Saudi ArabiaDepartment of Pharmacology, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City (USsC), Sadat City 22857, EgyptThe isolation and assessment of the active constituents in polar and non-polar crude extracts of <i>Saussurea costus</i> roots as antifungal agents, against <i>Candida albicans</i> and non-<i>C</i>. <i>albicans</i> (NAC) species, was the aim of this current investigation. The SEM “Scanning electron microscopy” imaging provided potential action modes of <i>n</i>-hexane extract (<i>nh</i>hE) toward <i>Candida</i> spp., whereas the TLC-DB “Thin layer chromatography-direct bioautography” was employed for detecting the anticandidal compounds. <i>nh</i>hE had the greatest biocidal activity against all strains and clinical isolates of <i>Candida</i> spp. with maximum zones of inhibition. SEM revealed the occurrence of irregular, dense inclusions of <i>C. albicans</i> cell walls after treatment with <i>nh</i>hE for 12 h. Complete morphological distortions with lysed membranes and deterioration signs appeared in most treated cells of <i>C. parapsilosis</i>. The most effectual compound with anticandidal activity was isolated using TLC-BD and identified as sesquiterpene by GC/MS analysis. The infra-red analysis revealed the presence of lactone ring stretching vibrations at 1766.72 cm<sup>−1</sup>. The anticandidal activity of <i>nh</i>hE of <i>S. costus</i> roots was confirmed from the results, and the treated cotton fabrics with <i>nh</i>hE of <i>S. costus</i> possessed observable activity against <i>C. albicans</i>. Data could recommend the practical usage of <i>S. costus</i> extracts, particularly <i>nh</i>hE, as influential natural bioactive sources for combating pathogenic <i>Candida</i> spp.https://www.mdpi.com/2079-6382/11/3/327anticandidal<i>S. costus</i> rootsdirect bioautographyGC/MS |
| spellingShingle | Melad F. Soliman Youssria M. Shetaia Ahmed A. Tayel Alaa M. Munshi Fuad A. Alatawi Mohammed A. Alsieni Mahmoud A. Al-Saman Exploring the Antifungal Activity and Action of <i>Saussurea costus</i> Root Extracts against <i>Candida albicans</i> and Non-<i>albicans</i> Species Antibiotics anticandidal <i>S. costus</i> roots direct bioautography GC/MS |
| title | Exploring the Antifungal Activity and Action of <i>Saussurea costus</i> Root Extracts against <i>Candida albicans</i> and Non-<i>albicans</i> Species |
| title_full | Exploring the Antifungal Activity and Action of <i>Saussurea costus</i> Root Extracts against <i>Candida albicans</i> and Non-<i>albicans</i> Species |
| title_fullStr | Exploring the Antifungal Activity and Action of <i>Saussurea costus</i> Root Extracts against <i>Candida albicans</i> and Non-<i>albicans</i> Species |
| title_full_unstemmed | Exploring the Antifungal Activity and Action of <i>Saussurea costus</i> Root Extracts against <i>Candida albicans</i> and Non-<i>albicans</i> Species |
| title_short | Exploring the Antifungal Activity and Action of <i>Saussurea costus</i> Root Extracts against <i>Candida albicans</i> and Non-<i>albicans</i> Species |
| title_sort | exploring the antifungal activity and action of i saussurea costus i root extracts against i candida albicans i and non i albicans i species |
| topic | anticandidal <i>S. costus</i> roots direct bioautography GC/MS |
| url | https://www.mdpi.com/2079-6382/11/3/327 |
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