Lipid Production of <i>Schizochytrium</i> sp. HBW10 Isolated from Coastal Waters of Northern China Cultivated in Food Waste Hydrolysate
Marine oleaginous thraustochytrids have attracted increasing attention for their great potential in producing high-value active metabolites using various industrial and agricultural waste. Food waste containing abundant nutrients is considered as an excellent feedstock for microbial fermentation. In...
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MDPI AG
2023-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/11/2714 |
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| author | Xiaofang Li Xinping Yu Qian Liu Yong Zhang Qiuzhen Wang |
| author_facet | Xiaofang Li Xinping Yu Qian Liu Yong Zhang Qiuzhen Wang |
| author_sort | Xiaofang Li |
| collection | DOAJ |
| description | Marine oleaginous thraustochytrids have attracted increasing attention for their great potential in producing high-value active metabolites using various industrial and agricultural waste. Food waste containing abundant nutrients is considered as an excellent feedstock for microbial fermentation. In this study, a thraustochytrid strain <i>Schizochytrium</i> sp. HBW10 was isolated from a water column in Bohai Bay in Northern China for the first time. Further lipid production characteristics of <i>S.</i> sp. HBW10 were investigated utilizing sulfuric acid hydrolysate of food waste (FWH) from two different restaurants (FWH1 and FWH2) with the initial pH value adjusted by NaOH or NaHCO<sub>3</sub>. Results showed that the highest concentration of total fatty acids (TFAs) was observed in FWH2 medium with the 50% content level on the fifth day, reaching up to 0.34 g/L. A higher initial pH promoted the growth and saturated fatty acid (SFA) accumulation of <i>S.</i> sp. HBW10, achieving nearly 100% of the sum of saturated and monounsaturated fatty acids (SMUFAs) in TFAs with initial pH7 and pH8 in FWH1 medium. This work demonstrates a possible way for lipid production by thraustochytrids using food waste hydrolysate with a higher initial pH (pH7~pH8) adjusted by NaHCO<sub>3</sub>. |
| first_indexed | 2024-03-09T16:35:29Z |
| format | Article |
| id | doaj.art-a4641dd0521d4fd9af939a281ddb4ac8 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:35:29Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a4641dd0521d4fd9af939a281ddb4ac82023-11-24T14:57:00ZengMDPI AGMicroorganisms2076-26072023-11-011111271410.3390/microorganisms11112714Lipid Production of <i>Schizochytrium</i> sp. HBW10 Isolated from Coastal Waters of Northern China Cultivated in Food Waste HydrolysateXiaofang Li0Xinping Yu1Qian Liu2Yong Zhang3Qiuzhen Wang4Ocean College, Hebei Agricultural University, Qinhuangdao 066000, ChinaOcean College, Hebei Agricultural University, Qinhuangdao 066000, ChinaOcean College, Hebei Agricultural University, Qinhuangdao 066000, ChinaMarine Environment Monitoring Central Station of Qinhuangdao, SOA, Qinhuangdao 066002, ChinaOcean College, Hebei Agricultural University, Qinhuangdao 066000, ChinaMarine oleaginous thraustochytrids have attracted increasing attention for their great potential in producing high-value active metabolites using various industrial and agricultural waste. Food waste containing abundant nutrients is considered as an excellent feedstock for microbial fermentation. In this study, a thraustochytrid strain <i>Schizochytrium</i> sp. HBW10 was isolated from a water column in Bohai Bay in Northern China for the first time. Further lipid production characteristics of <i>S.</i> sp. HBW10 were investigated utilizing sulfuric acid hydrolysate of food waste (FWH) from two different restaurants (FWH1 and FWH2) with the initial pH value adjusted by NaOH or NaHCO<sub>3</sub>. Results showed that the highest concentration of total fatty acids (TFAs) was observed in FWH2 medium with the 50% content level on the fifth day, reaching up to 0.34 g/L. A higher initial pH promoted the growth and saturated fatty acid (SFA) accumulation of <i>S.</i> sp. HBW10, achieving nearly 100% of the sum of saturated and monounsaturated fatty acids (SMUFAs) in TFAs with initial pH7 and pH8 in FWH1 medium. This work demonstrates a possible way for lipid production by thraustochytrids using food waste hydrolysate with a higher initial pH (pH7~pH8) adjusted by NaHCO<sub>3</sub>.https://www.mdpi.com/2076-2607/11/11/2714thraustochytrid<i>Schizochytrium</i>food waste hydrolysateinitial pHlipid |
| spellingShingle | Xiaofang Li Xinping Yu Qian Liu Yong Zhang Qiuzhen Wang Lipid Production of <i>Schizochytrium</i> sp. HBW10 Isolated from Coastal Waters of Northern China Cultivated in Food Waste Hydrolysate Microorganisms thraustochytrid <i>Schizochytrium</i> food waste hydrolysate initial pH lipid |
| title | Lipid Production of <i>Schizochytrium</i> sp. HBW10 Isolated from Coastal Waters of Northern China Cultivated in Food Waste Hydrolysate |
| title_full | Lipid Production of <i>Schizochytrium</i> sp. HBW10 Isolated from Coastal Waters of Northern China Cultivated in Food Waste Hydrolysate |
| title_fullStr | Lipid Production of <i>Schizochytrium</i> sp. HBW10 Isolated from Coastal Waters of Northern China Cultivated in Food Waste Hydrolysate |
| title_full_unstemmed | Lipid Production of <i>Schizochytrium</i> sp. HBW10 Isolated from Coastal Waters of Northern China Cultivated in Food Waste Hydrolysate |
| title_short | Lipid Production of <i>Schizochytrium</i> sp. HBW10 Isolated from Coastal Waters of Northern China Cultivated in Food Waste Hydrolysate |
| title_sort | lipid production of i schizochytrium i sp hbw10 isolated from coastal waters of northern china cultivated in food waste hydrolysate |
| topic | thraustochytrid <i>Schizochytrium</i> food waste hydrolysate initial pH lipid |
| url | https://www.mdpi.com/2076-2607/11/11/2714 |
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