Purification of Capsular Polysaccharides of Streptococcus pneumoniae: Traditional and New Methods
Pneumonia caused by Streptococcus pneumoniae is a major bacterial disease responsible for many deaths worldwide each year and is particularly dangerous in children under 5 years old and adults over 50. The capsular polysaccharide (CPS) constitutes the outermost layer of the bacterial cell and is the...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2018.00145/full |
| _version_ | 1811235679851511808 |
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| author | Victor Morais Valerie Dee Norma Suárez |
| author_facet | Victor Morais Valerie Dee Norma Suárez |
| author_sort | Victor Morais |
| collection | DOAJ |
| description | Pneumonia caused by Streptococcus pneumoniae is a major bacterial disease responsible for many deaths worldwide each year and is particularly dangerous in children under 5 years old and adults over 50. The capsular polysaccharide (CPS) constitutes the outermost layer of the bacterial cell and is the main virulence factor. Regardless of whether pharmaceutical agents are composed of CPS alone or protein-conjugated CPS, CPS purification is essential for the development of vaccines against S. pneumoniae. These vaccines are effective and safe but remain quite expensive. This review describes the methods currently available for CPS purification. Advances in CPS purification methods are aimed at improvements in quality and yield and, above all, process simplification. |
| first_indexed | 2024-04-12T11:56:10Z |
| format | Article |
| id | doaj.art-a464787e879241fe939b6ed5babb3392 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-12T11:56:10Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-a464787e879241fe939b6ed5babb33922022-12-22T03:34:00ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852018-10-01610.3389/fbioe.2018.00145384494Purification of Capsular Polysaccharides of Streptococcus pneumoniae: Traditional and New MethodsVictor MoraisValerie DeeNorma SuárezPneumonia caused by Streptococcus pneumoniae is a major bacterial disease responsible for many deaths worldwide each year and is particularly dangerous in children under 5 years old and adults over 50. The capsular polysaccharide (CPS) constitutes the outermost layer of the bacterial cell and is the main virulence factor. Regardless of whether pharmaceutical agents are composed of CPS alone or protein-conjugated CPS, CPS purification is essential for the development of vaccines against S. pneumoniae. These vaccines are effective and safe but remain quite expensive. This review describes the methods currently available for CPS purification. Advances in CPS purification methods are aimed at improvements in quality and yield and, above all, process simplification.https://www.frontiersin.org/article/10.3389/fbioe.2018.00145/fullcapsular polysaccharidesStreptococcus pneumoniaepurificationethanol precipitationvaccine |
| spellingShingle | Victor Morais Valerie Dee Norma Suárez Purification of Capsular Polysaccharides of Streptococcus pneumoniae: Traditional and New Methods Frontiers in Bioengineering and Biotechnology capsular polysaccharides Streptococcus pneumoniae purification ethanol precipitation vaccine |
| title | Purification of Capsular Polysaccharides of Streptococcus pneumoniae: Traditional and New Methods |
| title_full | Purification of Capsular Polysaccharides of Streptococcus pneumoniae: Traditional and New Methods |
| title_fullStr | Purification of Capsular Polysaccharides of Streptococcus pneumoniae: Traditional and New Methods |
| title_full_unstemmed | Purification of Capsular Polysaccharides of Streptococcus pneumoniae: Traditional and New Methods |
| title_short | Purification of Capsular Polysaccharides of Streptococcus pneumoniae: Traditional and New Methods |
| title_sort | purification of capsular polysaccharides of streptococcus pneumoniae traditional and new methods |
| topic | capsular polysaccharides Streptococcus pneumoniae purification ethanol precipitation vaccine |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2018.00145/full |
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